Strain Fitness in Escherichia coli ECRC62 around BNILDI_14705

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntugd and wzzB are separated by 145 nucleotideswzzB and hisIE are separated by 95 nucleotideshisIE and hisF overlap by 7 nucleotides BNILDI_14700: ugd - UDP-glucose 6-dehydrogenase, at 2,970,460 to 2,971,626 ugd BNILDI_14705: wzzB - LPS O-antigen chain length determinant protein WzzB, at 2,971,772 to 2,972,749 wzzB BNILDI_14710: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,972,845 to 2,973,456 hisIE BNILDI_14715: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 2,973,450 to 2,974,226 hisF Position (kb) 2971 2972 2973Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2970.930 kb on + strand, within ugdat 2970.931 kb on - strand, within ugdat 2971.017 kb on - strand, within ugdat 2971.060 kb on - strand, within ugdat 2971.087 kb on + strand, within ugdat 2971.088 kb on - strand, within ugdat 2971.088 kb on - strand, within ugdat 2971.109 kb on - strand, within ugdat 2971.171 kb on - strand, within ugdat 2971.252 kb on + strand, within ugdat 2971.253 kb on - strand, within ugdat 2971.363 kb on + strand, within ugdat 2971.393 kb on - strand, within ugdat 2971.411 kb on - strand, within ugdat 2971.431 kb on + strand, within ugdat 2971.549 kb on - strandat 2971.786 kb on + strandat 2971.786 kb on + strandat 2971.839 kb on + strandat 2971.842 kb on + strandat 2971.842 kb on + strandat 2971.890 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.013 kb on - strand, within wzzBat 2972.055 kb on + strand, within wzzBat 2972.055 kb on + strand, within wzzBat 2972.056 kb on - strand, within wzzBat 2972.177 kb on + strand, within wzzBat 2972.178 kb on - strand, within wzzBat 2972.178 kb on - strand, within wzzBat 2972.223 kb on - strand, within wzzBat 2972.259 kb on + strand, within wzzBat 2972.449 kb on + strand, within wzzBat 2972.449 kb on + strand, within wzzBat 2972.450 kb on - strand, within wzzBat 2972.452 kb on + strand, within wzzBat 2972.452 kb on + strand, within wzzBat 2972.453 kb on - strand, within wzzBat 2972.531 kb on + strand, within wzzBat 2972.606 kb on + strand, within wzzBat 2972.607 kb on - strand, within wzzBat 2972.629 kb on + strand, within wzzBat 2972.659 kb on + strandat 2972.659 kb on + strandat 2972.735 kb on + strandat 2972.735 kb on + strandat 2972.924 kb on + strand, within hisIEat 2972.924 kb on + strand, within hisIEat 2972.925 kb on - strand, within hisIEat 2972.925 kb on - strand, within hisIEat 2972.968 kb on + strand, within hisIEat 2972.968 kb on + strand, within hisIEat 2973.057 kb on - strand, within hisIEat 2973.228 kb on + strand, within hisIEat 2973.236 kb on - strand, within hisIEat 2973.348 kb on + strand, within hisIEat 2973.349 kb on - strand, within hisIEat 2973.351 kb on - strand, within hisIEat 2973.487 kb on + strandat 2973.496 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,970,930 + ugd BNILDI_14700 0.40 +1.0
2,970,931 - ugd BNILDI_14700 0.40 -2.0
2,971,017 - ugd BNILDI_14700 0.48 +0.3
2,971,060 - ugd BNILDI_14700 0.51 +0.7
2,971,087 + ugd BNILDI_14700 0.54 -0.2
2,971,088 - ugd BNILDI_14700 0.54 -0.1
2,971,088 - ugd BNILDI_14700 0.54 -0.4
2,971,109 - ugd BNILDI_14700 0.56 -0.3
2,971,171 - ugd BNILDI_14700 0.61 +0.2
2,971,252 + ugd BNILDI_14700 0.68 -0.4
2,971,253 - ugd BNILDI_14700 0.68 -2.4
2,971,363 + ugd BNILDI_14700 0.77 -1.8
2,971,393 - ugd BNILDI_14700 0.80 +0.6
2,971,411 - ugd BNILDI_14700 0.81 +1.9
2,971,431 + ugd BNILDI_14700 0.83 -2.2
2,971,549 - -0.6
2,971,786 + +1.6
2,971,786 + +0.3
2,971,839 + +0.6
2,971,842 + -0.1
2,971,842 + +0.1
2,971,890 + wzzB BNILDI_14705 0.12 -0.6
2,972,010 + wzzB BNILDI_14705 0.24 -0.0
2,972,010 + wzzB BNILDI_14705 0.24 +0.6
2,972,010 + wzzB BNILDI_14705 0.24 -2.0
2,972,010 + wzzB BNILDI_14705 0.24 +0.8
2,972,012 + wzzB BNILDI_14705 0.25 -0.1
2,972,012 + wzzB BNILDI_14705 0.25 +0.3
2,972,012 + wzzB BNILDI_14705 0.25 +0.2
2,972,013 - wzzB BNILDI_14705 0.25 +2.4
2,972,055 + wzzB BNILDI_14705 0.29 +0.3
2,972,055 + wzzB BNILDI_14705 0.29 -0.0
2,972,056 - wzzB BNILDI_14705 0.29 -0.6
2,972,177 + wzzB BNILDI_14705 0.41 +0.5
2,972,178 - wzzB BNILDI_14705 0.42 +0.2
2,972,178 - wzzB BNILDI_14705 0.42 +0.6
2,972,223 - wzzB BNILDI_14705 0.46 +0.3
2,972,259 + wzzB BNILDI_14705 0.50 +0.6
2,972,449 + wzzB BNILDI_14705 0.69 +0.5
2,972,449 + wzzB BNILDI_14705 0.69 -0.2
2,972,450 - wzzB BNILDI_14705 0.69 +2.0
2,972,452 + wzzB BNILDI_14705 0.70 +2.5
2,972,452 + wzzB BNILDI_14705 0.70 +0.2
2,972,453 - wzzB BNILDI_14705 0.70 -0.2
2,972,531 + wzzB BNILDI_14705 0.78 -0.7
2,972,606 + wzzB BNILDI_14705 0.85 -0.8
2,972,607 - wzzB BNILDI_14705 0.85 +0.5
2,972,629 + wzzB BNILDI_14705 0.88 -0.1
2,972,659 + -3.9
2,972,659 + -0.3
2,972,735 + +0.3
2,972,735 + -0.2
2,972,924 + hisIE BNILDI_14710 0.13 +0.0
2,972,924 + hisIE BNILDI_14710 0.13 -1.1
2,972,925 - hisIE BNILDI_14710 0.13 -1.9
2,972,925 - hisIE BNILDI_14710 0.13 -0.6
2,972,968 + hisIE BNILDI_14710 0.20 +0.1
2,972,968 + hisIE BNILDI_14710 0.20 +0.1
2,973,057 - hisIE BNILDI_14710 0.35 +1.0
2,973,228 + hisIE BNILDI_14710 0.63 -0.1
2,973,236 - hisIE BNILDI_14710 0.64 +0.4
2,973,348 + hisIE BNILDI_14710 0.82 -3.1
2,973,349 - hisIE BNILDI_14710 0.82 +0.2
2,973,351 - hisIE BNILDI_14710 0.83 -0.5
2,973,487 + +0.5
2,973,496 - -0.1

Or see this region's nucleotide sequence