Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaE and wcaF are separated by 15 nucleotides wcaF and BNILDI_14585 are separated by 25 nucleotides BNILDI_14585 and fcl are separated by 2 nucleotides fcl and gmm overlap by 1 nucleotides
BNILDI_14575: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 2,944,919 to 2,945,665
wcaE
BNILDI_14580: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 2,945,681 to 2,946,229
wcaF
BNILDI_14585: BNILDI_14585 - GDP-mannose 4,6-dehydratase, at 2,946,255 to 2,947,376
_14585
BNILDI_14590: fcl - GDP-L-fucose synthase, at 2,947,379 to 2,948,344
fcl
BNILDI_14595: gmm - GDP-mannose mannosyl hydrolase, at 2,948,344 to 2,948,826
gmm
Position (kb)
2946
2947
2948 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2945.653 kb on + strand at 2945.878 kb on + strand, within wcaF at 2945.993 kb on + strand, within wcaF at 2946.249 kb on + strand at 2946.249 kb on + strand at 2946.252 kb on + strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.253 kb on - strand at 2946.347 kb on + strand at 2946.351 kb on - strand at 2946.439 kb on - strand, within BNILDI_14585 at 2946.439 kb on - strand, within BNILDI_14585 at 2946.439 kb on - strand, within BNILDI_14585 at 2946.455 kb on + strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585 at 2946.456 kb on - strand, within BNILDI_14585 at 2946.786 kb on + strand, within BNILDI_14585 at 2946.786 kb on + strand, within BNILDI_14585 at 2946.787 kb on - strand, within BNILDI_14585 at 2946.787 kb on - strand, within BNILDI_14585 at 2946.787 kb on - strand, within BNILDI_14585 at 2947.024 kb on - strand, within BNILDI_14585 at 2947.148 kb on + strand, within BNILDI_14585 at 2947.299 kb on - strand at 2947.374 kb on + strand at 2947.375 kb on - strand at 2947.400 kb on - strand at 2947.477 kb on - strand, within fcl at 2947.544 kb on - strand, within fcl at 2947.709 kb on + strand, within fcl at 2947.784 kb on + strand, within fcl at 2947.785 kb on - strand, within fcl at 2947.827 kb on - strand, within fcl at 2947.827 kb on - strand, within fcl at 2947.827 kb on - strand, within fcl at 2947.827 kb on - strand, within fcl at 2948.203 kb on + strand, within fcl at 2948.303 kb on + strand at 2948.303 kb on + strand at 2948.304 kb on - strand at 2948.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,945,653 + -0.6 2,945,878 + wcaF BNILDI_14580 0.36 -0.5 2,945,993 + wcaF BNILDI_14580 0.57 +0.9 2,946,249 + +0.0 2,946,249 + -0.6 2,946,252 + +0.1 2,946,253 - -0.4 2,946,253 - -0.5 2,946,253 - -3.0 2,946,253 - -3.6 2,946,347 + +0.3 2,946,351 - +0.1 2,946,439 - BNILDI_14585 0.16 -0.8 2,946,439 - BNILDI_14585 0.16 -0.4 2,946,439 - BNILDI_14585 0.16 -0.8 2,946,455 + BNILDI_14585 0.18 -1.5 2,946,456 - BNILDI_14585 0.18 -0.2 2,946,456 - BNILDI_14585 0.18 +0.1 2,946,456 - BNILDI_14585 0.18 -0.5 2,946,786 + BNILDI_14585 0.47 +0.2 2,946,786 + BNILDI_14585 0.47 -0.2 2,946,787 - BNILDI_14585 0.47 +0.1 2,946,787 - BNILDI_14585 0.47 -0.0 2,946,787 - BNILDI_14585 0.47 +0.6 2,947,024 - BNILDI_14585 0.69 -2.7 2,947,148 + BNILDI_14585 0.80 +0.5 2,947,299 - -1.2 2,947,374 + +0.1 2,947,375 - -1.5 2,947,400 - -0.7 2,947,477 - fcl BNILDI_14590 0.10 +0.8 2,947,544 - fcl BNILDI_14590 0.17 -0.1 2,947,709 + fcl BNILDI_14590 0.34 +0.2 2,947,784 + fcl BNILDI_14590 0.42 -0.1 2,947,785 - fcl BNILDI_14590 0.42 -0.0 2,947,827 - fcl BNILDI_14590 0.46 +0.5 2,947,827 - fcl BNILDI_14590 0.46 -1.6 2,947,827 - fcl BNILDI_14590 0.46 +0.2 2,947,827 - fcl BNILDI_14590 0.46 -0.2 2,948,203 + fcl BNILDI_14590 0.85 -0.6 2,948,303 + +0.2 2,948,303 + -0.2 2,948,304 - +0.6 2,948,304 - -0.1
Or see this region's nucleotide sequence