Strain Fitness in Escherichia coli ECRC62 around BNILDI_14585

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaE and wcaF are separated by 15 nucleotideswcaF and BNILDI_14585 are separated by 25 nucleotidesBNILDI_14585 and fcl are separated by 2 nucleotidesfcl and gmm overlap by 1 nucleotides BNILDI_14575: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 2,944,919 to 2,945,665 wcaE BNILDI_14580: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 2,945,681 to 2,946,229 wcaF BNILDI_14585: BNILDI_14585 - GDP-mannose 4,6-dehydratase, at 2,946,255 to 2,947,376 _14585 BNILDI_14590: fcl - GDP-L-fucose synthase, at 2,947,379 to 2,948,344 fcl BNILDI_14595: gmm - GDP-mannose mannosyl hydrolase, at 2,948,344 to 2,948,826 gmm Position (kb) 2946 2947 2948Strain fitness (log2 ratio) -3 -2 -1 0 1at 2945.653 kb on + strandat 2945.878 kb on + strand, within wcaFat 2945.993 kb on + strand, within wcaFat 2946.249 kb on + strandat 2946.249 kb on + strandat 2946.252 kb on + strandat 2946.253 kb on - strandat 2946.253 kb on - strandat 2946.253 kb on - strandat 2946.253 kb on - strandat 2946.347 kb on + strandat 2946.351 kb on - strandat 2946.439 kb on - strand, within BNILDI_14585at 2946.439 kb on - strand, within BNILDI_14585at 2946.439 kb on - strand, within BNILDI_14585at 2946.455 kb on + strand, within BNILDI_14585at 2946.456 kb on - strand, within BNILDI_14585at 2946.456 kb on - strand, within BNILDI_14585at 2946.456 kb on - strand, within BNILDI_14585at 2946.786 kb on + strand, within BNILDI_14585at 2946.786 kb on + strand, within BNILDI_14585at 2946.787 kb on - strand, within BNILDI_14585at 2946.787 kb on - strand, within BNILDI_14585at 2946.787 kb on - strand, within BNILDI_14585at 2947.024 kb on - strand, within BNILDI_14585at 2947.148 kb on + strand, within BNILDI_14585at 2947.299 kb on - strandat 2947.374 kb on + strandat 2947.375 kb on - strandat 2947.400 kb on - strandat 2947.477 kb on - strand, within fclat 2947.544 kb on - strand, within fclat 2947.709 kb on + strand, within fclat 2947.784 kb on + strand, within fclat 2947.785 kb on - strand, within fclat 2947.827 kb on - strand, within fclat 2947.827 kb on - strand, within fclat 2947.827 kb on - strand, within fclat 2947.827 kb on - strand, within fclat 2948.203 kb on + strand, within fclat 2948.303 kb on + strandat 2948.303 kb on + strandat 2948.304 kb on - strandat 2948.304 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,945,653 + -0.6
2,945,878 + wcaF BNILDI_14580 0.36 -0.5
2,945,993 + wcaF BNILDI_14580 0.57 +0.9
2,946,249 + +0.0
2,946,249 + -0.6
2,946,252 + +0.1
2,946,253 - -0.4
2,946,253 - -0.5
2,946,253 - -3.0
2,946,253 - -3.6
2,946,347 + +0.3
2,946,351 - +0.1
2,946,439 - BNILDI_14585 0.16 -0.8
2,946,439 - BNILDI_14585 0.16 -0.4
2,946,439 - BNILDI_14585 0.16 -0.8
2,946,455 + BNILDI_14585 0.18 -1.5
2,946,456 - BNILDI_14585 0.18 -0.2
2,946,456 - BNILDI_14585 0.18 +0.1
2,946,456 - BNILDI_14585 0.18 -0.5
2,946,786 + BNILDI_14585 0.47 +0.2
2,946,786 + BNILDI_14585 0.47 -0.2
2,946,787 - BNILDI_14585 0.47 +0.1
2,946,787 - BNILDI_14585 0.47 -0.0
2,946,787 - BNILDI_14585 0.47 +0.6
2,947,024 - BNILDI_14585 0.69 -2.7
2,947,148 + BNILDI_14585 0.80 +0.5
2,947,299 - -1.2
2,947,374 + +0.1
2,947,375 - -1.5
2,947,400 - -0.7
2,947,477 - fcl BNILDI_14590 0.10 +0.8
2,947,544 - fcl BNILDI_14590 0.17 -0.1
2,947,709 + fcl BNILDI_14590 0.34 +0.2
2,947,784 + fcl BNILDI_14590 0.42 -0.1
2,947,785 - fcl BNILDI_14590 0.42 -0.0
2,947,827 - fcl BNILDI_14590 0.46 +0.5
2,947,827 - fcl BNILDI_14590 0.46 -1.6
2,947,827 - fcl BNILDI_14590 0.46 +0.2
2,947,827 - fcl BNILDI_14590 0.46 -0.2
2,948,203 + fcl BNILDI_14590 0.85 -0.6
2,948,303 + +0.2
2,948,303 + -0.2
2,948,304 - +0.6
2,948,304 - -0.1

Or see this region's nucleotide sequence