Strain Fitness in Escherichia coli ECRC62 around BNILDI_14560

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaA and wcaB are separated by 2 nucleotideswcaB and wcaC overlap by 4 nucleotides BNILDI_14555: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 2,941,172 to 2,942,011 wcaA BNILDI_14560: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 2,942,014 to 2,942,502 wcaB BNILDI_14565: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 2,942,499 to 2,943,716 wcaC Position (kb) 2942 2943Strain fitness (log2 ratio) -3 -2 -1 0 1at 2941.040 kb on - strandat 2941.133 kb on - strandat 2941.171 kb on + strandat 2941.172 kb on - strandat 2941.172 kb on - strandat 2941.200 kb on - strandat 2941.200 kb on - strandat 2941.202 kb on - strandat 2941.265 kb on + strand, within wcaAat 2941.266 kb on - strand, within wcaAat 2941.328 kb on + strand, within wcaAat 2941.329 kb on - strand, within wcaAat 2941.329 kb on - strand, within wcaAat 2941.362 kb on - strand, within wcaAat 2941.403 kb on - strand, within wcaAat 2941.433 kb on + strand, within wcaAat 2941.434 kb on - strand, within wcaAat 2941.434 kb on - strand, within wcaAat 2941.434 kb on - strand, within wcaAat 2941.434 kb on - strand, within wcaAat 2941.604 kb on + strand, within wcaAat 2941.605 kb on - strand, within wcaAat 2941.622 kb on + strand, within wcaAat 2941.623 kb on - strand, within wcaAat 2941.623 kb on - strand, within wcaAat 2941.654 kb on + strand, within wcaAat 2941.715 kb on + strand, within wcaAat 2941.745 kb on + strand, within wcaAat 2941.745 kb on + strand, within wcaAat 2941.842 kb on - strand, within wcaAat 2942.223 kb on + strand, within wcaBat 2942.223 kb on + strand, within wcaBat 2942.224 kb on - strand, within wcaBat 2942.256 kb on + strand, within wcaBat 2942.304 kb on + strand, within wcaBat 2942.376 kb on + strand, within wcaBat 2942.673 kb on - strand, within wcaCat 2942.752 kb on + strand, within wcaCat 2942.829 kb on - strand, within wcaCat 2942.984 kb on - strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.988 kb on - strand, within wcaCat 2943.046 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.323 kb on + strand, within wcaCat 2943.454 kb on - strand, within wcaC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,941,040 - -2.0
2,941,133 - -0.1
2,941,171 + +0.9
2,941,172 - -0.2
2,941,172 - +0.8
2,941,200 - -1.3
2,941,200 - +0.0
2,941,202 - -1.0
2,941,265 + wcaA BNILDI_14555 0.11 +0.2
2,941,266 - wcaA BNILDI_14555 0.11 +0.0
2,941,328 + wcaA BNILDI_14555 0.19 +0.0
2,941,329 - wcaA BNILDI_14555 0.19 -1.9
2,941,329 - wcaA BNILDI_14555 0.19 +0.6
2,941,362 - wcaA BNILDI_14555 0.23 +1.1
2,941,403 - wcaA BNILDI_14555 0.28 +0.5
2,941,433 + wcaA BNILDI_14555 0.31 -1.0
2,941,434 - wcaA BNILDI_14555 0.31 -2.1
2,941,434 - wcaA BNILDI_14555 0.31 -0.3
2,941,434 - wcaA BNILDI_14555 0.31 -0.4
2,941,434 - wcaA BNILDI_14555 0.31 -0.1
2,941,604 + wcaA BNILDI_14555 0.51 +0.3
2,941,605 - wcaA BNILDI_14555 0.52 -0.1
2,941,622 + wcaA BNILDI_14555 0.54 -1.3
2,941,623 - wcaA BNILDI_14555 0.54 -3.4
2,941,623 - wcaA BNILDI_14555 0.54 -0.6
2,941,654 + wcaA BNILDI_14555 0.57 -0.8
2,941,715 + wcaA BNILDI_14555 0.65 +0.2
2,941,745 + wcaA BNILDI_14555 0.68 +0.2
2,941,745 + wcaA BNILDI_14555 0.68 -0.2
2,941,842 - wcaA BNILDI_14555 0.80 +0.1
2,942,223 + wcaB BNILDI_14560 0.43 -0.0
2,942,223 + wcaB BNILDI_14560 0.43 -2.2
2,942,224 - wcaB BNILDI_14560 0.43 -1.4
2,942,256 + wcaB BNILDI_14560 0.49 +0.6
2,942,304 + wcaB BNILDI_14560 0.59 +0.0
2,942,376 + wcaB BNILDI_14560 0.74 -0.3
2,942,673 - wcaC BNILDI_14565 0.14 +0.4
2,942,752 + wcaC BNILDI_14565 0.21 -0.8
2,942,829 - wcaC BNILDI_14565 0.27 -0.4
2,942,984 - wcaC BNILDI_14565 0.40 -0.1
2,942,987 + wcaC BNILDI_14565 0.40 -1.1
2,942,987 + wcaC BNILDI_14565 0.40 +0.9
2,942,987 + wcaC BNILDI_14565 0.40 +0.3
2,942,987 + wcaC BNILDI_14565 0.40 +1.4
2,942,988 - wcaC BNILDI_14565 0.40 +0.1
2,943,046 + wcaC BNILDI_14565 0.45 -0.5
2,943,152 + wcaC BNILDI_14565 0.54 +0.2
2,943,152 + wcaC BNILDI_14565 0.54 -0.7
2,943,323 + wcaC BNILDI_14565 0.68 +1.4
2,943,454 - wcaC BNILDI_14565 0.78 +0.0

Or see this region's nucleotide sequence