Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ibsA and terY are separated by 220 nucleotides terY and pphC are separated by 77 nucleotides pphC and yegI overlap by 4 nucleotides
BNILDI_14480: ibsA - Small toxic protein IbsA, at 2,921,874 to 2,921,933
ibsA
BNILDI_14485: terY - Uncharacterized protein YegL, at 2,922,154 to 2,922,813
terY
BNILDI_14490: pphC - Serine/threonine-protein phosphatase 3, at 2,922,891 to 2,923,571
pphC
BNILDI_14495: yegI - protein kinase YegI, at 2,923,568 to 2,925,508
yegI
Position (kb)
2922
2923
2924 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2921.915 kb on + strand, within ibsA at 2921.929 kb on - strand at 2921.931 kb on + strand at 2921.931 kb on + strand at 2921.932 kb on - strand at 2921.932 kb on - strand at 2921.999 kb on + strand at 2921.999 kb on + strand at 2922.038 kb on - strand at 2922.065 kb on - strand at 2922.092 kb on + strand at 2922.262 kb on - strand, within terY at 2922.265 kb on - strand, within terY at 2922.274 kb on - strand, within terY at 2922.514 kb on + strand, within terY at 2922.607 kb on - strand, within terY at 2922.829 kb on - strand at 2922.868 kb on + strand at 2922.868 kb on + strand at 2922.869 kb on - strand at 2923.011 kb on + strand, within pphC at 2923.012 kb on - strand, within pphC at 2923.012 kb on - strand, within pphC at 2923.012 kb on - strand, within pphC at 2923.013 kb on + strand, within pphC at 2923.014 kb on - strand, within pphC at 2923.049 kb on + strand, within pphC at 2923.267 kb on - strand, within pphC at 2923.267 kb on - strand, within pphC at 2923.267 kb on - strand, within pphC at 2923.274 kb on + strand, within pphC at 2923.275 kb on - strand, within pphC at 2923.391 kb on + strand, within pphC at 2923.391 kb on + strand, within pphC at 2923.392 kb on - strand, within pphC at 2923.392 kb on - strand, within pphC at 2923.400 kb on + strand, within pphC at 2923.415 kb on - strand, within pphC at 2923.475 kb on - strand, within pphC at 2923.539 kb on - strand at 2923.581 kb on + strand at 2923.581 kb on + strand at 2923.582 kb on - strand at 2923.582 kb on - strand at 2923.585 kb on + strand at 2923.585 kb on + strand at 2923.585 kb on + strand at 2923.650 kb on - strand at 2923.650 kb on - strand at 2923.689 kb on - strand at 2923.766 kb on + strand, within yegI at 2923.767 kb on - strand, within yegI at 2923.767 kb on - strand, within yegI at 2923.767 kb on - strand, within yegI at 2923.847 kb on - strand, within yegI at 2923.862 kb on - strand, within yegI at 2923.862 kb on - strand, within yegI at 2923.868 kb on + strand, within yegI at 2923.868 kb on + strand, within yegI at 2923.869 kb on - strand, within yegI at 2923.869 kb on - strand, within yegI at 2923.920 kb on + strand, within yegI at 2923.920 kb on + strand, within yegI at 2923.926 kb on - strand, within yegI at 2924.245 kb on + strand, within yegI at 2924.437 kb on + strand, within yegI at 2924.542 kb on + strand, within yegI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,921,915 + ibsA BNILDI_14480 0.68 -0.2 2,921,929 - +0.1 2,921,931 + +0.8 2,921,931 + +0.0 2,921,932 - -0.2 2,921,932 - +0.1 2,921,999 + +0.0 2,921,999 + -0.7 2,922,038 - -1.0 2,922,065 - +0.9 2,922,092 + -1.0 2,922,262 - terY BNILDI_14485 0.16 -0.9 2,922,265 - terY BNILDI_14485 0.17 -0.1 2,922,274 - terY BNILDI_14485 0.18 +0.2 2,922,514 + terY BNILDI_14485 0.55 +0.7 2,922,607 - terY BNILDI_14485 0.69 +0.8 2,922,829 - +0.8 2,922,868 + +0.5 2,922,868 + +0.3 2,922,869 - -0.1 2,923,011 + pphC BNILDI_14490 0.18 -1.4 2,923,012 - pphC BNILDI_14490 0.18 +0.1 2,923,012 - pphC BNILDI_14490 0.18 +2.2 2,923,012 - pphC BNILDI_14490 0.18 +2.9 2,923,013 + pphC BNILDI_14490 0.18 +0.5 2,923,014 - pphC BNILDI_14490 0.18 -0.2 2,923,049 + pphC BNILDI_14490 0.23 -1.4 2,923,267 - pphC BNILDI_14490 0.55 +0.6 2,923,267 - pphC BNILDI_14490 0.55 +0.0 2,923,267 - pphC BNILDI_14490 0.55 -0.1 2,923,274 + pphC BNILDI_14490 0.56 -2.2 2,923,275 - pphC BNILDI_14490 0.56 -0.2 2,923,391 + pphC BNILDI_14490 0.73 -0.1 2,923,391 + pphC BNILDI_14490 0.73 +0.4 2,923,392 - pphC BNILDI_14490 0.74 +0.9 2,923,392 - pphC BNILDI_14490 0.74 -0.0 2,923,400 + pphC BNILDI_14490 0.75 -0.6 2,923,415 - pphC BNILDI_14490 0.77 -0.2 2,923,475 - pphC BNILDI_14490 0.86 +0.2 2,923,539 - -1.2 2,923,581 + -0.4 2,923,581 + +0.2 2,923,582 - +0.1 2,923,582 - -0.1 2,923,585 + +0.4 2,923,585 + -0.1 2,923,585 + -0.4 2,923,650 - -0.7 2,923,650 - +0.4 2,923,689 - +1.0 2,923,766 + yegI BNILDI_14495 0.10 -0.5 2,923,767 - yegI BNILDI_14495 0.10 -0.2 2,923,767 - yegI BNILDI_14495 0.10 -2.2 2,923,767 - yegI BNILDI_14495 0.10 -0.1 2,923,847 - yegI BNILDI_14495 0.14 +1.3 2,923,862 - yegI BNILDI_14495 0.15 +0.7 2,923,862 - yegI BNILDI_14495 0.15 -0.3 2,923,868 + yegI BNILDI_14495 0.15 -2.7 2,923,868 + yegI BNILDI_14495 0.15 -1.7 2,923,869 - yegI BNILDI_14495 0.16 +0.8 2,923,869 - yegI BNILDI_14495 0.16 +0.3 2,923,920 + yegI BNILDI_14495 0.18 -0.5 2,923,920 + yegI BNILDI_14495 0.18 +0.1 2,923,926 - yegI BNILDI_14495 0.18 -0.3 2,924,245 + yegI BNILDI_14495 0.35 -0.6 2,924,437 + yegI BNILDI_14495 0.45 +1.4 2,924,542 + yegI BNILDI_14495 0.50 -0.2
Or see this region's nucleotide sequence