Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_13375 and nuoA are separated by 262 nucleotides nuoA and nuoB are separated by 15 nucleotides nuoB and nuoC are separated by 93 nucleotides
BNILDI_13375: BNILDI_13375 - hypothetical protein, at 2,679,566 to 2,679,730
_13375
BNILDI_13380: nuoA - NADH-quinone oxidoreductase subunit NuoA, at 2,679,993 to 2,680,436
nuoA
BNILDI_13385: nuoB - NADH-quinone oxidoreductase subunit NuoB, at 2,680,452 to 2,681,114
nuoB
BNILDI_13390: nuoC - NADH-quinone oxidoreductase subunit C/D, at 2,681,208 to 2,683,010
nuoC
Position (kb)
2680
2681
2682 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2679.479 kb on - strand at 2679.871 kb on + strand at 2679.891 kb on + strand at 2679.998 kb on + strand at 2679.999 kb on - strand at 2679.999 kb on - strand at 2680.197 kb on + strand, within nuoA at 2680.242 kb on + strand, within nuoA at 2680.242 kb on + strand, within nuoA at 2680.243 kb on - strand, within nuoA at 2680.395 kb on - strand at 2680.449 kb on - strand at 2680.645 kb on - strand, within nuoB at 2680.858 kb on - strand, within nuoB at 2680.858 kb on - strand, within nuoB at 2680.911 kb on + strand, within nuoB at 2680.911 kb on + strand, within nuoB at 2680.912 kb on - strand, within nuoB at 2680.944 kb on + strand, within nuoB at 2680.944 kb on + strand, within nuoB at 2681.096 kb on + strand at 2681.150 kb on - strand at 2681.219 kb on + strand at 2681.220 kb on - strand at 2681.419 kb on - strand, within nuoC at 2681.419 kb on - strand, within nuoC at 2681.419 kb on - strand, within nuoC at 2681.616 kb on + strand, within nuoC at 2681.616 kb on + strand, within nuoC at 2681.835 kb on - strand, within nuoC at 2682.067 kb on - strand, within nuoC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,679,479 - -0.0 2,679,871 + -1.9 2,679,891 + -2.6 2,679,998 + -1.9 2,679,999 - -2.4 2,679,999 - -2.5 2,680,197 + nuoA BNILDI_13380 0.46 -3.2 2,680,242 + nuoA BNILDI_13380 0.56 -2.3 2,680,242 + nuoA BNILDI_13380 0.56 -1.6 2,680,243 - nuoA BNILDI_13380 0.56 -3.3 2,680,395 - -1.2 2,680,449 - -3.0 2,680,645 - nuoB BNILDI_13385 0.29 -2.2 2,680,858 - nuoB BNILDI_13385 0.61 -2.9 2,680,858 - nuoB BNILDI_13385 0.61 -1.9 2,680,911 + nuoB BNILDI_13385 0.69 -3.0 2,680,911 + nuoB BNILDI_13385 0.69 -1.0 2,680,912 - nuoB BNILDI_13385 0.69 -1.1 2,680,944 + nuoB BNILDI_13385 0.74 -1.1 2,680,944 + nuoB BNILDI_13385 0.74 -2.9 2,681,096 + +1.7 2,681,150 - -0.9 2,681,219 + -1.9 2,681,220 - -1.3 2,681,419 - nuoC BNILDI_13390 0.12 -2.3 2,681,419 - nuoC BNILDI_13390 0.12 -2.3 2,681,419 - nuoC BNILDI_13390 0.12 -1.5 2,681,616 + nuoC BNILDI_13390 0.23 -0.3 2,681,616 + nuoC BNILDI_13390 0.23 -1.6 2,681,835 - nuoC BNILDI_13390 0.35 -1.4 2,682,067 - nuoC BNILDI_13390 0.48 -3.0
Or see this region's nucleotide sequence