Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purC and vapB are separated by 115 nucleotides vapB and vapC overlap by 1 nucleotides vapC and ypfJ are separated by 146 nucleotides
BNILDI_12400: purC - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 2,488,530 to 2,489,243
purC
BNILDI_12405: vapB - toxin-antitoxin system antitoxin VapB, at 2,489,359 to 2,489,625
vapB
BNILDI_12410: vapC - VapC toxin family PIN domain ribonuclease, at 2,489,625 to 2,490,023
vapC
BNILDI_12415: ypfJ - Uncharacterized protein YpfJ, at 2,490,170 to 2,491,033
ypfJ
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2488.859 kb on + strand, within purC at 2488.859 kb on + strand, within purC at 2488.935 kb on + strand, within purC at 2488.936 kb on - strand, within purC at 2488.995 kb on + strand, within purC at 2489.077 kb on - strand, within purC at 2489.202 kb on + strand at 2489.203 kb on - strand at 2489.203 kb on - strand at 2489.231 kb on - strand at 2489.267 kb on - strand at 2489.287 kb on - strand at 2489.308 kb on + strand at 2489.313 kb on + strand at 2489.313 kb on + strand at 2489.323 kb on + strand at 2489.651 kb on + strand at 2489.666 kb on + strand, within vapC at 2489.669 kb on + strand, within vapC at 2489.670 kb on - strand, within vapC at 2489.684 kb on - strand, within vapC at 2489.728 kb on + strand, within vapC at 2489.728 kb on + strand, within vapC at 2489.728 kb on + strand, within vapC at 2489.729 kb on - strand, within vapC at 2489.753 kb on + strand, within vapC at 2489.753 kb on + strand, within vapC at 2489.757 kb on + strand, within vapC at 2489.758 kb on - strand, within vapC at 2489.861 kb on + strand, within vapC at 2490.035 kb on - strand at 2490.041 kb on - strand at 2490.055 kb on - strand at 2490.057 kb on - strand at 2490.057 kb on - strand at 2490.058 kb on + strand at 2490.131 kb on + strand at 2490.131 kb on + strand at 2490.144 kb on - strand at 2490.144 kb on - strand at 2490.144 kb on - strand at 2490.153 kb on - strand at 2490.154 kb on + strand at 2490.155 kb on - strand at 2490.166 kb on - strand at 2490.166 kb on - strand at 2490.170 kb on - strand at 2490.170 kb on - strand at 2490.170 kb on - strand at 2490.170 kb on - strand at 2490.314 kb on + strand, within ypfJ at 2490.317 kb on + strand, within ypfJ at 2490.317 kb on + strand, within ypfJ at 2490.563 kb on + strand, within ypfJ at 2490.587 kb on + strand, within ypfJ at 2490.587 kb on + strand, within ypfJ at 2490.588 kb on - strand, within ypfJ at 2490.605 kb on + strand, within ypfJ at 2490.605 kb on + strand, within ypfJ at 2490.606 kb on - strand, within ypfJ at 2490.657 kb on - strand, within ypfJ at 2490.657 kb on - strand, within ypfJ at 2490.699 kb on + strand, within ypfJ at 2490.814 kb on + strand, within ypfJ at 2490.814 kb on + strand, within ypfJ at 2490.815 kb on - strand, within ypfJ at 2490.815 kb on - strand, within ypfJ at 2490.817 kb on + strand, within ypfJ at 2490.817 kb on + strand, within ypfJ at 2490.817 kb on + strand, within ypfJ at 2490.817 kb on + strand, within ypfJ at 2490.818 kb on - strand, within ypfJ at 2490.818 kb on - strand, within ypfJ at 2490.924 kb on + strand, within ypfJ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,488,859 + purC BNILDI_12400 0.46 -4.0 2,488,859 + purC BNILDI_12400 0.46 -2.3 2,488,935 + purC BNILDI_12400 0.57 -2.9 2,488,936 - purC BNILDI_12400 0.57 -2.4 2,488,995 + purC BNILDI_12400 0.65 -2.7 2,489,077 - purC BNILDI_12400 0.77 -2.9 2,489,202 + -4.3 2,489,203 - -5.0 2,489,203 - -4.7 2,489,231 - -6.3 2,489,267 - -0.2 2,489,287 - +0.8 2,489,308 + +0.9 2,489,313 + +0.0 2,489,313 + -0.3 2,489,323 + -0.4 2,489,651 + +0.1 2,489,666 + vapC BNILDI_12410 0.10 +0.4 2,489,669 + vapC BNILDI_12410 0.11 +0.3 2,489,670 - vapC BNILDI_12410 0.11 -3.1 2,489,684 - vapC BNILDI_12410 0.15 +0.2 2,489,728 + vapC BNILDI_12410 0.26 -0.2 2,489,728 + vapC BNILDI_12410 0.26 -0.0 2,489,728 + vapC BNILDI_12410 0.26 -0.5 2,489,729 - vapC BNILDI_12410 0.26 -0.5 2,489,753 + vapC BNILDI_12410 0.32 -1.2 2,489,753 + vapC BNILDI_12410 0.32 +0.2 2,489,757 + vapC BNILDI_12410 0.33 +1.3 2,489,758 - vapC BNILDI_12410 0.33 -0.3 2,489,861 + vapC BNILDI_12410 0.59 -0.9 2,490,035 - -2.4 2,490,041 - +0.4 2,490,055 - -0.2 2,490,057 - +1.0 2,490,057 - -0.4 2,490,058 + -0.1 2,490,131 + -1.4 2,490,131 + +0.0 2,490,144 - -0.3 2,490,144 - +1.1 2,490,144 - +0.0 2,490,153 - +0.4 2,490,154 + +0.1 2,490,155 - +0.2 2,490,166 - -0.4 2,490,166 - -0.1 2,490,170 - +0.5 2,490,170 - -0.5 2,490,170 - +0.3 2,490,170 - -0.8 2,490,314 + ypfJ BNILDI_12415 0.17 -0.1 2,490,317 + ypfJ BNILDI_12415 0.17 -1.0 2,490,317 + ypfJ BNILDI_12415 0.17 +0.4 2,490,563 + ypfJ BNILDI_12415 0.45 -0.2 2,490,587 + ypfJ BNILDI_12415 0.48 -1.0 2,490,587 + ypfJ BNILDI_12415 0.48 +1.6 2,490,588 - ypfJ BNILDI_12415 0.48 -1.1 2,490,605 + ypfJ BNILDI_12415 0.50 +0.0 2,490,605 + ypfJ BNILDI_12415 0.50 -0.3 2,490,606 - ypfJ BNILDI_12415 0.50 -0.3 2,490,657 - ypfJ BNILDI_12415 0.56 -0.5 2,490,657 - ypfJ BNILDI_12415 0.56 +0.1 2,490,699 + ypfJ BNILDI_12415 0.61 +0.2 2,490,814 + ypfJ BNILDI_12415 0.75 -0.3 2,490,814 + ypfJ BNILDI_12415 0.75 -0.3 2,490,815 - ypfJ BNILDI_12415 0.75 +0.1 2,490,815 - ypfJ BNILDI_12415 0.75 -0.4 2,490,817 + ypfJ BNILDI_12415 0.75 -0.6 2,490,817 + ypfJ BNILDI_12415 0.75 +0.7 2,490,817 + ypfJ BNILDI_12415 0.75 -2.8 2,490,817 + ypfJ BNILDI_12415 0.75 +0.3 2,490,818 - ypfJ BNILDI_12415 0.75 +0.3 2,490,818 - ypfJ BNILDI_12415 0.75 +1.3 2,490,924 + ypfJ BNILDI_12415 0.87 -0.7
Or see this region's nucleotide sequence