Strain Fitness in Escherichia coli ECRC62 around BNILDI_12410

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurC and vapB are separated by 115 nucleotidesvapB and vapC overlap by 1 nucleotidesvapC and ypfJ are separated by 146 nucleotides BNILDI_12400: purC - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 2,488,530 to 2,489,243 purC BNILDI_12405: vapB - toxin-antitoxin system antitoxin VapB, at 2,489,359 to 2,489,625 vapB BNILDI_12410: vapC - VapC toxin family PIN domain ribonuclease, at 2,489,625 to 2,490,023 vapC BNILDI_12415: ypfJ - Uncharacterized protein YpfJ, at 2,490,170 to 2,491,033 ypfJ Position (kb) 2489 2490 2491Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2488.859 kb on + strand, within purCat 2488.859 kb on + strand, within purCat 2488.935 kb on + strand, within purCat 2488.936 kb on - strand, within purCat 2488.995 kb on + strand, within purCat 2489.077 kb on - strand, within purCat 2489.202 kb on + strandat 2489.203 kb on - strandat 2489.203 kb on - strandat 2489.231 kb on - strandat 2489.267 kb on - strandat 2489.287 kb on - strandat 2489.308 kb on + strandat 2489.313 kb on + strandat 2489.313 kb on + strandat 2489.323 kb on + strandat 2489.651 kb on + strandat 2489.666 kb on + strand, within vapCat 2489.669 kb on + strand, within vapCat 2489.670 kb on - strand, within vapCat 2489.684 kb on - strand, within vapCat 2489.728 kb on + strand, within vapCat 2489.728 kb on + strand, within vapCat 2489.728 kb on + strand, within vapCat 2489.729 kb on - strand, within vapCat 2489.753 kb on + strand, within vapCat 2489.753 kb on + strand, within vapCat 2489.757 kb on + strand, within vapCat 2489.758 kb on - strand, within vapCat 2489.861 kb on + strand, within vapCat 2490.035 kb on - strandat 2490.041 kb on - strandat 2490.055 kb on - strandat 2490.057 kb on - strandat 2490.057 kb on - strandat 2490.058 kb on + strandat 2490.131 kb on + strandat 2490.131 kb on + strandat 2490.144 kb on - strandat 2490.144 kb on - strandat 2490.144 kb on - strandat 2490.153 kb on - strandat 2490.154 kb on + strandat 2490.155 kb on - strandat 2490.166 kb on - strandat 2490.166 kb on - strandat 2490.170 kb on - strandat 2490.170 kb on - strandat 2490.170 kb on - strandat 2490.170 kb on - strandat 2490.314 kb on + strand, within ypfJat 2490.317 kb on + strand, within ypfJat 2490.317 kb on + strand, within ypfJat 2490.563 kb on + strand, within ypfJat 2490.587 kb on + strand, within ypfJat 2490.587 kb on + strand, within ypfJat 2490.588 kb on - strand, within ypfJat 2490.605 kb on + strand, within ypfJat 2490.605 kb on + strand, within ypfJat 2490.606 kb on - strand, within ypfJat 2490.657 kb on - strand, within ypfJat 2490.657 kb on - strand, within ypfJat 2490.699 kb on + strand, within ypfJat 2490.814 kb on + strand, within ypfJat 2490.814 kb on + strand, within ypfJat 2490.815 kb on - strand, within ypfJat 2490.815 kb on - strand, within ypfJat 2490.817 kb on + strand, within ypfJat 2490.817 kb on + strand, within ypfJat 2490.817 kb on + strand, within ypfJat 2490.817 kb on + strand, within ypfJat 2490.818 kb on - strand, within ypfJat 2490.818 kb on - strand, within ypfJat 2490.924 kb on + strand, within ypfJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,488,859 + purC BNILDI_12400 0.46 -4.0
2,488,859 + purC BNILDI_12400 0.46 -2.3
2,488,935 + purC BNILDI_12400 0.57 -2.9
2,488,936 - purC BNILDI_12400 0.57 -2.4
2,488,995 + purC BNILDI_12400 0.65 -2.7
2,489,077 - purC BNILDI_12400 0.77 -2.9
2,489,202 + -4.3
2,489,203 - -5.0
2,489,203 - -4.7
2,489,231 - -6.3
2,489,267 - -0.2
2,489,287 - +0.8
2,489,308 + +0.9
2,489,313 + +0.0
2,489,313 + -0.3
2,489,323 + -0.4
2,489,651 + +0.1
2,489,666 + vapC BNILDI_12410 0.10 +0.4
2,489,669 + vapC BNILDI_12410 0.11 +0.3
2,489,670 - vapC BNILDI_12410 0.11 -3.1
2,489,684 - vapC BNILDI_12410 0.15 +0.2
2,489,728 + vapC BNILDI_12410 0.26 -0.2
2,489,728 + vapC BNILDI_12410 0.26 -0.0
2,489,728 + vapC BNILDI_12410 0.26 -0.5
2,489,729 - vapC BNILDI_12410 0.26 -0.5
2,489,753 + vapC BNILDI_12410 0.32 -1.2
2,489,753 + vapC BNILDI_12410 0.32 +0.2
2,489,757 + vapC BNILDI_12410 0.33 +1.3
2,489,758 - vapC BNILDI_12410 0.33 -0.3
2,489,861 + vapC BNILDI_12410 0.59 -0.9
2,490,035 - -2.4
2,490,041 - +0.4
2,490,055 - -0.2
2,490,057 - +1.0
2,490,057 - -0.4
2,490,058 + -0.1
2,490,131 + -1.4
2,490,131 + +0.0
2,490,144 - -0.3
2,490,144 - +1.1
2,490,144 - +0.0
2,490,153 - +0.4
2,490,154 + +0.1
2,490,155 - +0.2
2,490,166 - -0.4
2,490,166 - -0.1
2,490,170 - +0.5
2,490,170 - -0.5
2,490,170 - +0.3
2,490,170 - -0.8
2,490,314 + ypfJ BNILDI_12415 0.17 -0.1
2,490,317 + ypfJ BNILDI_12415 0.17 -1.0
2,490,317 + ypfJ BNILDI_12415 0.17 +0.4
2,490,563 + ypfJ BNILDI_12415 0.45 -0.2
2,490,587 + ypfJ BNILDI_12415 0.48 -1.0
2,490,587 + ypfJ BNILDI_12415 0.48 +1.6
2,490,588 - ypfJ BNILDI_12415 0.48 -1.1
2,490,605 + ypfJ BNILDI_12415 0.50 +0.0
2,490,605 + ypfJ BNILDI_12415 0.50 -0.3
2,490,606 - ypfJ BNILDI_12415 0.50 -0.3
2,490,657 - ypfJ BNILDI_12415 0.56 -0.5
2,490,657 - ypfJ BNILDI_12415 0.56 +0.1
2,490,699 + ypfJ BNILDI_12415 0.61 +0.2
2,490,814 + ypfJ BNILDI_12415 0.75 -0.3
2,490,814 + ypfJ BNILDI_12415 0.75 -0.3
2,490,815 - ypfJ BNILDI_12415 0.75 +0.1
2,490,815 - ypfJ BNILDI_12415 0.75 -0.4
2,490,817 + ypfJ BNILDI_12415 0.75 -0.6
2,490,817 + ypfJ BNILDI_12415 0.75 +0.7
2,490,817 + ypfJ BNILDI_12415 0.75 -2.8
2,490,817 + ypfJ BNILDI_12415 0.75 +0.3
2,490,818 - ypfJ BNILDI_12415 0.75 +0.3
2,490,818 - ypfJ BNILDI_12415 0.75 +1.3
2,490,924 + ypfJ BNILDI_12415 0.87 -0.7

Or see this region's nucleotide sequence