Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfE and BNILDI_12345 are separated by 11 nucleotides BNILDI_12345 and BNILDI_12350 are separated by 32 nucleotides BNILDI_12350 and BNILDI_12355 overlap by 25 nucleotides BNILDI_12355 and hyfC are separated by 16 nucleotides
BNILDI_12340: hyfE - hydrogenase 4 membrane subunit, at 2,479,445 to 2,480,095
hyfE
BNILDI_12345: BNILDI_12345 - Hydrogenase 4 subunit D, at 2,480,107 to 2,480,475
_12345
BNILDI_12350: BNILDI_12350 - Hydrogenase 4 subunit D, at 2,480,508 to 2,481,251
_12350
BNILDI_12355: BNILDI_12355 - hydantoin racemase, at 2,481,227 to 2,481,337
_12355
BNILDI_12360: hyfC - hydrogenase 4 subunit HyfC, at 2,481,354 to 2,482,322
hyfC
Position (kb)
2480
2481
2482 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2479.560 kb on - strand, within hyfE at 2479.560 kb on - strand, within hyfE at 2479.701 kb on - strand, within hyfE at 2479.784 kb on + strand, within hyfE at 2479.785 kb on - strand, within hyfE at 2479.863 kb on + strand, within hyfE at 2479.863 kb on + strand, within hyfE at 2479.967 kb on - strand, within hyfE at 2479.967 kb on - strand, within hyfE at 2480.072 kb on - strand at 2480.161 kb on + strand, within BNILDI_12345 at 2480.256 kb on + strand, within BNILDI_12345 at 2480.256 kb on + strand, within BNILDI_12345 at 2480.492 kb on + strand at 2480.509 kb on + strand at 2480.510 kb on - strand at 2480.710 kb on + strand, within BNILDI_12350 at 2480.736 kb on + strand, within BNILDI_12350 at 2480.737 kb on - strand, within BNILDI_12350 at 2480.829 kb on - strand, within BNILDI_12350 at 2480.841 kb on + strand, within BNILDI_12350 at 2480.842 kb on - strand, within BNILDI_12350 at 2480.872 kb on + strand, within BNILDI_12350 at 2480.876 kb on - strand, within BNILDI_12350 at 2480.949 kb on + strand, within BNILDI_12350 at 2480.950 kb on - strand, within BNILDI_12350 at 2481.056 kb on - strand, within BNILDI_12350 at 2481.105 kb on - strand, within BNILDI_12350 at 2481.191 kb on - strand at 2481.646 kb on + strand, within hyfC at 2481.973 kb on + strand, within hyfC at 2482.065 kb on + strand, within hyfC at 2482.105 kb on - strand, within hyfC at 2482.105 kb on - strand, within hyfC at 2482.106 kb on + strand, within hyfC at 2482.106 kb on + strand, within hyfC at 2482.107 kb on - strand, within hyfC at 2482.107 kb on - strand, within hyfC at 2482.107 kb on - strand, within hyfC at 2482.107 kb on - strand, within hyfC at 2482.107 kb on - strand, within hyfC at 2482.187 kb on + strand, within hyfC at 2482.197 kb on + strand, within hyfC at 2482.197 kb on + strand, within hyfC at 2482.212 kb on - strand, within hyfC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,479,560 - hyfE BNILDI_12340 0.18 +0.6 2,479,560 - hyfE BNILDI_12340 0.18 +1.7 2,479,701 - hyfE BNILDI_12340 0.39 -0.1 2,479,784 + hyfE BNILDI_12340 0.52 -0.6 2,479,785 - hyfE BNILDI_12340 0.52 +0.0 2,479,863 + hyfE BNILDI_12340 0.64 -1.7 2,479,863 + hyfE BNILDI_12340 0.64 +0.2 2,479,967 - hyfE BNILDI_12340 0.80 -1.0 2,479,967 - hyfE BNILDI_12340 0.80 +0.2 2,480,072 - +0.1 2,480,161 + BNILDI_12345 0.15 -1.7 2,480,256 + BNILDI_12345 0.40 +0.1 2,480,256 + BNILDI_12345 0.40 +1.5 2,480,492 + -0.1 2,480,509 + -0.1 2,480,510 - -0.4 2,480,710 + BNILDI_12350 0.27 -2.2 2,480,736 + BNILDI_12350 0.31 +0.3 2,480,737 - BNILDI_12350 0.31 +0.1 2,480,829 - BNILDI_12350 0.43 -0.1 2,480,841 + BNILDI_12350 0.45 -0.1 2,480,842 - BNILDI_12350 0.45 +0.5 2,480,872 + BNILDI_12350 0.49 -0.2 2,480,876 - BNILDI_12350 0.49 -0.0 2,480,949 + BNILDI_12350 0.59 +0.3 2,480,950 - BNILDI_12350 0.59 +0.2 2,481,056 - BNILDI_12350 0.74 -0.7 2,481,105 - BNILDI_12350 0.80 +0.0 2,481,191 - -0.1 2,481,646 + hyfC BNILDI_12360 0.30 +1.3 2,481,973 + hyfC BNILDI_12360 0.64 +0.6 2,482,065 + hyfC BNILDI_12360 0.73 +0.1 2,482,105 - hyfC BNILDI_12360 0.78 -0.4 2,482,105 - hyfC BNILDI_12360 0.78 -0.5 2,482,106 + hyfC BNILDI_12360 0.78 +0.2 2,482,106 + hyfC BNILDI_12360 0.78 -2.8 2,482,107 - hyfC BNILDI_12360 0.78 +0.2 2,482,107 - hyfC BNILDI_12360 0.78 +0.7 2,482,107 - hyfC BNILDI_12360 0.78 -0.1 2,482,107 - hyfC BNILDI_12360 0.78 +0.4 2,482,107 - hyfC BNILDI_12360 0.78 +0.6 2,482,187 + hyfC BNILDI_12360 0.86 +0.0 2,482,197 + hyfC BNILDI_12360 0.87 +0.7 2,482,197 + hyfC BNILDI_12360 0.87 -1.3 2,482,212 - hyfC BNILDI_12360 0.89 -0.2
Or see this region's nucleotide sequence