Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeF and ppx are separated by 38 nucleotides ppx and ppk1 are separated by 4 nucleotides
BNILDI_12250: pdeF - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,457,408 to 2,459,651
pdeF
BNILDI_12255: ppx - exopolyphosphatase, at 2,459,690 to 2,461,231
ppx
BNILDI_12260: ppk1 - polyphosphate kinase 1, at 2,461,236 to 2,463,302
ppk1
Position (kb)
2459
2460
2461
2462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2458.746 kb on + strand, within pdeF at 2458.747 kb on - strand, within pdeF at 2458.747 kb on - strand, within pdeF at 2458.774 kb on - strand, within pdeF at 2458.774 kb on - strand, within pdeF at 2458.797 kb on - strand, within pdeF at 2458.838 kb on + strand, within pdeF at 2458.961 kb on + strand, within pdeF at 2458.977 kb on + strand, within pdeF at 2459.099 kb on + strand, within pdeF at 2459.099 kb on + strand, within pdeF at 2459.099 kb on + strand, within pdeF at 2459.100 kb on - strand, within pdeF at 2459.100 kb on - strand, within pdeF at 2459.100 kb on - strand, within pdeF at 2459.100 kb on - strand, within pdeF at 2459.100 kb on - strand, within pdeF at 2459.153 kb on + strand, within pdeF at 2459.153 kb on + strand, within pdeF at 2459.157 kb on - strand, within pdeF at 2459.220 kb on - strand, within pdeF at 2459.246 kb on + strand, within pdeF at 2459.433 kb on - strand at 2459.462 kb on - strand at 2459.517 kb on + strand at 2459.518 kb on - strand at 2459.518 kb on - strand at 2459.569 kb on - strand at 2459.570 kb on + strand at 2459.570 kb on + strand at 2459.591 kb on + strand at 2459.758 kb on - strand at 2459.804 kb on - strand at 2459.900 kb on + strand, within ppx at 2459.901 kb on - strand, within ppx at 2459.903 kb on + strand, within ppx at 2459.918 kb on + strand, within ppx at 2459.925 kb on - strand, within ppx at 2459.951 kb on - strand, within ppx at 2459.959 kb on + strand, within ppx at 2459.959 kb on + strand, within ppx at 2460.072 kb on + strand, within ppx at 2460.202 kb on - strand, within ppx at 2460.325 kb on - strand, within ppx at 2460.342 kb on - strand, within ppx at 2460.377 kb on + strand, within ppx at 2460.706 kb on - strand, within ppx at 2460.706 kb on - strand, within ppx at 2460.755 kb on - strand, within ppx at 2460.838 kb on + strand, within ppx at 2460.838 kb on + strand, within ppx at 2460.974 kb on + strand, within ppx at 2460.975 kb on - strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.069 kb on + strand, within ppx at 2461.069 kb on + strand, within ppx at 2461.140 kb on - strand at 2461.152 kb on - strand at 2461.152 kb on - strand at 2461.216 kb on + strand at 2461.265 kb on - strand at 2461.270 kb on + strand at 2461.270 kb on + strand at 2461.271 kb on - strand at 2461.271 kb on - strand at 2461.374 kb on + strand at 2461.375 kb on - strand at 2461.375 kb on - strand at 2461.375 kb on - strand at 2461.538 kb on - strand, within ppk1 at 2461.550 kb on + strand, within ppk1 at 2461.655 kb on - strand, within ppk1 at 2461.688 kb on + strand, within ppk1 at 2461.689 kb on - strand, within ppk1 at 2461.693 kb on - strand, within ppk1 at 2461.693 kb on - strand, within ppk1 at 2461.749 kb on + strand, within ppk1 at 2461.750 kb on - strand, within ppk1 at 2461.760 kb on - strand, within ppk1 at 2461.794 kb on + strand, within ppk1 at 2461.795 kb on - strand, within ppk1 at 2461.838 kb on + strand, within ppk1 at 2462.027 kb on + strand, within ppk1 at 2462.028 kb on - strand, within ppk1 at 2462.073 kb on - strand, within ppk1 at 2462.124 kb on - strand, within ppk1 at 2462.130 kb on - strand, within ppk1
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,458,746 + pdeF BNILDI_12250 0.60 +0.1 2,458,747 - pdeF BNILDI_12250 0.60 +0.6 2,458,747 - pdeF BNILDI_12250 0.60 +0.1 2,458,774 - pdeF BNILDI_12250 0.61 -0.1 2,458,774 - pdeF BNILDI_12250 0.61 +0.1 2,458,797 - pdeF BNILDI_12250 0.62 -2.9 2,458,838 + pdeF BNILDI_12250 0.64 -0.4 2,458,961 + pdeF BNILDI_12250 0.69 +0.5 2,458,977 + pdeF BNILDI_12250 0.70 -0.2 2,459,099 + pdeF BNILDI_12250 0.75 -0.2 2,459,099 + pdeF BNILDI_12250 0.75 -0.3 2,459,099 + pdeF BNILDI_12250 0.75 +0.5 2,459,100 - pdeF BNILDI_12250 0.75 +0.2 2,459,100 - pdeF BNILDI_12250 0.75 -0.5 2,459,100 - pdeF BNILDI_12250 0.75 +0.3 2,459,100 - pdeF BNILDI_12250 0.75 +0.9 2,459,100 - pdeF BNILDI_12250 0.75 -0.2 2,459,153 + pdeF BNILDI_12250 0.78 -0.1 2,459,153 + pdeF BNILDI_12250 0.78 -2.2 2,459,157 - pdeF BNILDI_12250 0.78 -0.2 2,459,220 - pdeF BNILDI_12250 0.81 +2.1 2,459,246 + pdeF BNILDI_12250 0.82 -0.3 2,459,433 - -0.3 2,459,462 - +0.5 2,459,517 + -1.5 2,459,518 - -0.2 2,459,518 - +0.2 2,459,569 - -1.0 2,459,570 + +0.5 2,459,570 + -0.0 2,459,591 + -1.1 2,459,758 - -1.6 2,459,804 - +0.2 2,459,900 + ppx BNILDI_12255 0.14 +0.0 2,459,901 - ppx BNILDI_12255 0.14 +0.5 2,459,903 + ppx BNILDI_12255 0.14 +0.2 2,459,918 + ppx BNILDI_12255 0.15 -0.1 2,459,925 - ppx BNILDI_12255 0.15 +0.3 2,459,951 - ppx BNILDI_12255 0.17 -2.1 2,459,959 + ppx BNILDI_12255 0.17 -0.4 2,459,959 + ppx BNILDI_12255 0.17 -0.2 2,460,072 + ppx BNILDI_12255 0.25 +0.8 2,460,202 - ppx BNILDI_12255 0.33 +0.2 2,460,325 - ppx BNILDI_12255 0.41 -0.5 2,460,342 - ppx BNILDI_12255 0.42 -0.1 2,460,377 + ppx BNILDI_12255 0.45 +1.0 2,460,706 - ppx BNILDI_12255 0.66 -0.5 2,460,706 - ppx BNILDI_12255 0.66 +0.6 2,460,755 - ppx BNILDI_12255 0.69 -2.6 2,460,838 + ppx BNILDI_12255 0.74 -0.8 2,460,838 + ppx BNILDI_12255 0.74 +2.7 2,460,974 + ppx BNILDI_12255 0.83 +0.2 2,460,975 - ppx BNILDI_12255 0.83 +2.4 2,461,066 + ppx BNILDI_12255 0.89 -0.0 2,461,066 + ppx BNILDI_12255 0.89 +1.8 2,461,066 + ppx BNILDI_12255 0.89 -0.0 2,461,069 + ppx BNILDI_12255 0.89 +0.7 2,461,069 + ppx BNILDI_12255 0.89 +0.2 2,461,140 - -1.7 2,461,152 - -2.8 2,461,152 - +0.1 2,461,216 + -0.4 2,461,265 - -0.3 2,461,270 + -0.2 2,461,270 + -0.7 2,461,271 - -0.1 2,461,271 - +0.7 2,461,374 + +0.3 2,461,375 - -0.2 2,461,375 - -2.4 2,461,375 - -0.5 2,461,538 - ppk1 BNILDI_12260 0.15 -0.8 2,461,550 + ppk1 BNILDI_12260 0.15 -1.0 2,461,655 - ppk1 BNILDI_12260 0.20 -0.1 2,461,688 + ppk1 BNILDI_12260 0.22 -0.5 2,461,689 - ppk1 BNILDI_12260 0.22 -0.1 2,461,693 - ppk1 BNILDI_12260 0.22 -1.1 2,461,693 - ppk1 BNILDI_12260 0.22 -0.3 2,461,749 + ppk1 BNILDI_12260 0.25 -1.1 2,461,750 - ppk1 BNILDI_12260 0.25 -0.3 2,461,760 - ppk1 BNILDI_12260 0.25 +0.2 2,461,794 + ppk1 BNILDI_12260 0.27 -1.2 2,461,795 - ppk1 BNILDI_12260 0.27 +1.5 2,461,838 + ppk1 BNILDI_12260 0.29 +0.9 2,462,027 + ppk1 BNILDI_12260 0.38 +0.1 2,462,028 - ppk1 BNILDI_12260 0.38 +0.3 2,462,073 - ppk1 BNILDI_12260 0.40 -1.8 2,462,124 - ppk1 BNILDI_12260 0.43 -0.7 2,462,130 - ppk1 BNILDI_12260 0.43 -0.2
Or see this region's nucleotide sequence