Strain Fitness in Escherichia coli ECRC62 around BNILDI_12040

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaD and hcaB are separated by 9 nucleotideshcaB and hcaC overlap by 4 nucleotideshcaC and hcaF overlap by 1 nucleotideshcaF and hcaE overlap by 4 nucleotides BNILDI_12030: hcaD - phenylpropionate dioxygenase ferredoxin reductase subunit, at 2,412,314 to 2,413,516 hcaD BNILDI_12035: hcaB - 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase, at 2,413,526 to 2,414,338 hcaB BNILDI_12040: hcaC - bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit, at 2,414,335 to 2,414,655 hcaC BNILDI_12045: hcaF - 3-phenylpropionate/cinnamic acid dioxygenase subunit beta, at 2,414,655 to 2,415,173 hcaF BNILDI_12050: hcaE - 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha, at 2,415,170 to 2,416,531 hcaE Position (kb) 2414 2415Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2413.341 kb on - strand, within hcaDat 2413.393 kb on - strand, within hcaDat 2413.393 kb on - strand, within hcaDat 2413.495 kb on + strandat 2413.527 kb on + strandat 2413.641 kb on + strand, within hcaBat 2413.642 kb on - strand, within hcaBat 2413.777 kb on + strand, within hcaBat 2413.777 kb on + strand, within hcaBat 2413.988 kb on + strand, within hcaBat 2414.042 kb on - strand, within hcaBat 2414.042 kb on - strand, within hcaBat 2414.161 kb on + strand, within hcaBat 2414.179 kb on + strand, within hcaBat 2414.353 kb on + strandat 2414.394 kb on - strand, within hcaCat 2414.498 kb on + strand, within hcaCat 2414.549 kb on + strand, within hcaCat 2414.642 kb on + strandat 2414.642 kb on + strandat 2414.664 kb on + strandat 2414.664 kb on + strandat 2414.665 kb on - strandat 2414.665 kb on - strandat 2414.776 kb on + strand, within hcaFat 2414.776 kb on + strand, within hcaFat 2414.777 kb on - strand, within hcaFat 2414.777 kb on - strand, within hcaFat 2414.777 kb on - strand, within hcaFat 2414.777 kb on - strand, within hcaFat 2414.818 kb on + strand, within hcaFat 2414.838 kb on + strand, within hcaFat 2414.839 kb on - strand, within hcaFat 2414.880 kb on + strand, within hcaFat 2415.030 kb on - strand, within hcaFat 2415.050 kb on - strand, within hcaFat 2415.105 kb on + strand, within hcaFat 2415.106 kb on - strand, within hcaFat 2415.147 kb on + strandat 2415.147 kb on + strandat 2415.147 kb on + strandat 2415.147 kb on + strandat 2415.147 kb on + strandat 2415.148 kb on - strandat 2415.148 kb on - strandat 2415.290 kb on - strandat 2415.291 kb on + strandat 2415.292 kb on - strandat 2415.292 kb on - strandat 2415.295 kb on + strandat 2415.295 kb on + strandat 2415.295 kb on + strandat 2415.296 kb on - strandat 2415.296 kb on - strandat 2415.296 kb on - strandat 2415.296 kb on - strandat 2415.299 kb on - strandat 2415.613 kb on + strand, within hcaEat 2415.647 kb on + strand, within hcaE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,413,341 - hcaD BNILDI_12030 0.85 -1.3
2,413,393 - hcaD BNILDI_12030 0.90 -0.2
2,413,393 - hcaD BNILDI_12030 0.90 -0.5
2,413,495 + +0.6
2,413,527 + -0.8
2,413,641 + hcaB BNILDI_12035 0.14 +0.7
2,413,642 - hcaB BNILDI_12035 0.14 +0.0
2,413,777 + hcaB BNILDI_12035 0.31 -0.2
2,413,777 + hcaB BNILDI_12035 0.31 +0.7
2,413,988 + hcaB BNILDI_12035 0.57 +0.8
2,414,042 - hcaB BNILDI_12035 0.63 +0.4
2,414,042 - hcaB BNILDI_12035 0.63 -0.3
2,414,161 + hcaB BNILDI_12035 0.78 -1.6
2,414,179 + hcaB BNILDI_12035 0.80 -0.1
2,414,353 + -0.0
2,414,394 - hcaC BNILDI_12040 0.18 +0.2
2,414,498 + hcaC BNILDI_12040 0.51 -0.9
2,414,549 + hcaC BNILDI_12040 0.67 -0.0
2,414,642 + +1.3
2,414,642 + +0.6
2,414,664 + -1.0
2,414,664 + +0.3
2,414,665 - +0.1
2,414,665 - +0.2
2,414,776 + hcaF BNILDI_12045 0.23 +0.3
2,414,776 + hcaF BNILDI_12045 0.23 -0.0
2,414,777 - hcaF BNILDI_12045 0.24 +1.2
2,414,777 - hcaF BNILDI_12045 0.24 -0.1
2,414,777 - hcaF BNILDI_12045 0.24 -0.0
2,414,777 - hcaF BNILDI_12045 0.24 +0.2
2,414,818 + hcaF BNILDI_12045 0.31 -0.4
2,414,838 + hcaF BNILDI_12045 0.35 -0.0
2,414,839 - hcaF BNILDI_12045 0.35 +0.2
2,414,880 + hcaF BNILDI_12045 0.43 +0.0
2,415,030 - hcaF BNILDI_12045 0.72 +1.1
2,415,050 - hcaF BNILDI_12045 0.76 +0.4
2,415,105 + hcaF BNILDI_12045 0.87 -0.2
2,415,106 - hcaF BNILDI_12045 0.87 +1.0
2,415,147 + -0.4
2,415,147 + -0.4
2,415,147 + +0.6
2,415,147 + +1.0
2,415,147 + +0.0
2,415,148 - +1.1
2,415,148 - +0.1
2,415,290 - -1.9
2,415,291 + +2.8
2,415,292 - +0.0
2,415,292 - -0.1
2,415,295 + +0.5
2,415,295 + -1.8
2,415,295 + -0.8
2,415,296 - +0.0
2,415,296 - -0.3
2,415,296 - -0.0
2,415,296 - -0.1
2,415,299 - +0.3
2,415,613 + hcaE BNILDI_12050 0.33 -0.2
2,415,647 + hcaE BNILDI_12050 0.35 +0.1

Or see this region's nucleotide sequence