Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpD and BNILDI_11580 are separated by 90 nucleotides BNILDI_11580 and BNILDI_11585 are separated by 261 nucleotides BNILDI_11585 and BNILDI_11590 overlap by 32 nucleotides
BNILDI_11575: gpD - late control protein D, at 2,326,519 to 2,327,619
gpD
BNILDI_11580: BNILDI_11580 - transcriptional regulator, at 2,327,710 to 2,327,928
_11580
BNILDI_11585: BNILDI_11585 - hypothetical protein, at 2,328,190 to 2,328,363
_11585
BNILDI_11590: BNILDI_11590 - reverse transcriptase, at 2,328,332 to 2,329,105
_11590
Position (kb)
2328
2329 Strain fitness (log2 ratio)
-2
-1
0
1 at 2327.198 kb on + strand, within gpD at 2327.198 kb on + strand, within gpD at 2327.199 kb on - strand, within gpD at 2327.199 kb on - strand, within gpD at 2327.259 kb on + strand, within gpD at 2327.271 kb on + strand, within gpD at 2327.312 kb on - strand, within gpD at 2327.346 kb on + strand, within gpD at 2327.347 kb on - strand, within gpD at 2327.356 kb on + strand, within gpD at 2327.357 kb on - strand, within gpD at 2327.436 kb on - strand, within gpD at 2327.530 kb on - strand at 2327.789 kb on - strand, within BNILDI_11580 at 2327.809 kb on - strand, within BNILDI_11580 at 2327.809 kb on - strand, within BNILDI_11580 at 2327.820 kb on + strand, within BNILDI_11580 at 2327.904 kb on + strand, within BNILDI_11580 at 2328.012 kb on + strand at 2328.013 kb on - strand at 2328.029 kb on + strand at 2328.030 kb on - strand at 2328.030 kb on - strand at 2328.033 kb on + strand at 2328.034 kb on - strand at 2328.034 kb on - strand at 2328.034 kb on - strand at 2328.078 kb on + strand at 2328.133 kb on + strand at 2328.174 kb on + strand at 2328.175 kb on - strand at 2328.175 kb on - strand at 2328.196 kb on - strand at 2328.212 kb on + strand, within BNILDI_11585 at 2328.213 kb on - strand, within BNILDI_11585 at 2328.213 kb on - strand, within BNILDI_11585 at 2328.217 kb on - strand, within BNILDI_11585 at 2328.275 kb on + strand, within BNILDI_11585 at 2328.415 kb on + strand, within BNILDI_11590 at 2328.432 kb on + strand, within BNILDI_11590 at 2328.432 kb on + strand, within BNILDI_11590 at 2328.433 kb on - strand, within BNILDI_11590 at 2328.476 kb on + strand, within BNILDI_11590 at 2328.483 kb on + strand, within BNILDI_11590 at 2328.502 kb on + strand, within BNILDI_11590 at 2328.586 kb on + strand, within BNILDI_11590 at 2328.600 kb on + strand, within BNILDI_11590 at 2328.655 kb on - strand, within BNILDI_11590 at 2328.679 kb on + strand, within BNILDI_11590 at 2328.679 kb on + strand, within BNILDI_11590 at 2328.680 kb on - strand, within BNILDI_11590 at 2328.759 kb on + strand, within BNILDI_11590 at 2328.760 kb on - strand, within BNILDI_11590 at 2328.795 kb on + strand, within BNILDI_11590 at 2328.795 kb on + strand, within BNILDI_11590 at 2328.796 kb on - strand, within BNILDI_11590 at 2328.796 kb on - strand, within BNILDI_11590 at 2328.884 kb on - strand, within BNILDI_11590 at 2328.910 kb on + strand, within BNILDI_11590 at 2328.971 kb on - strand, within BNILDI_11590 at 2328.971 kb on - strand, within BNILDI_11590 at 2329.029 kb on - strand at 2329.044 kb on + strand at 2329.097 kb on - strand at 2329.103 kb on + strand at 2329.119 kb on + strand at 2329.119 kb on + strand at 2329.120 kb on - strand at 2329.120 kb on - strand at 2329.120 kb on - strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.122 kb on + strand at 2329.123 kb on - strand at 2329.269 kb on - strand at 2329.288 kb on - strand at 2329.331 kb on - strand at 2329.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,327,198 + gpD BNILDI_11575 0.62 -0.8 2,327,198 + gpD BNILDI_11575 0.62 -0.3 2,327,199 - gpD BNILDI_11575 0.62 +0.1 2,327,199 - gpD BNILDI_11575 0.62 +0.3 2,327,259 + gpD BNILDI_11575 0.67 +0.0 2,327,271 + gpD BNILDI_11575 0.68 -0.3 2,327,312 - gpD BNILDI_11575 0.72 +0.4 2,327,346 + gpD BNILDI_11575 0.75 +0.1 2,327,347 - gpD BNILDI_11575 0.75 +0.3 2,327,356 + gpD BNILDI_11575 0.76 +0.6 2,327,357 - gpD BNILDI_11575 0.76 +0.3 2,327,436 - gpD BNILDI_11575 0.83 +0.6 2,327,530 - +1.7 2,327,789 - BNILDI_11580 0.36 +0.3 2,327,809 - BNILDI_11580 0.45 -0.5 2,327,809 - BNILDI_11580 0.45 +0.1 2,327,820 + BNILDI_11580 0.50 +0.1 2,327,904 + BNILDI_11580 0.89 -0.2 2,328,012 + +1.2 2,328,013 - -2.0 2,328,029 + +0.1 2,328,030 - +0.2 2,328,030 - +0.5 2,328,033 + -0.7 2,328,034 - +0.5 2,328,034 - -0.2 2,328,034 - -0.5 2,328,078 + -0.2 2,328,133 + +0.8 2,328,174 + -0.7 2,328,175 - +0.8 2,328,175 - -0.6 2,328,196 - -1.0 2,328,212 + BNILDI_11585 0.13 -1.3 2,328,213 - BNILDI_11585 0.13 +0.6 2,328,213 - BNILDI_11585 0.13 +0.4 2,328,217 - BNILDI_11585 0.16 -1.8 2,328,275 + BNILDI_11585 0.49 -0.2 2,328,415 + BNILDI_11590 0.11 -0.1 2,328,432 + BNILDI_11590 0.13 -0.5 2,328,432 + BNILDI_11590 0.13 +1.0 2,328,433 - BNILDI_11590 0.13 +1.1 2,328,476 + BNILDI_11590 0.19 +0.0 2,328,483 + BNILDI_11590 0.20 +0.2 2,328,502 + BNILDI_11590 0.22 +0.2 2,328,586 + BNILDI_11590 0.33 -0.3 2,328,600 + BNILDI_11590 0.35 -0.4 2,328,655 - BNILDI_11590 0.42 +1.0 2,328,679 + BNILDI_11590 0.45 -0.1 2,328,679 + BNILDI_11590 0.45 -0.1 2,328,680 - BNILDI_11590 0.45 -1.7 2,328,759 + BNILDI_11590 0.55 +0.1 2,328,760 - BNILDI_11590 0.55 -0.3 2,328,795 + BNILDI_11590 0.60 -0.4 2,328,795 + BNILDI_11590 0.60 +0.3 2,328,796 - BNILDI_11590 0.60 +0.6 2,328,796 - BNILDI_11590 0.60 +1.4 2,328,884 - BNILDI_11590 0.71 +0.2 2,328,910 + BNILDI_11590 0.75 -0.0 2,328,971 - BNILDI_11590 0.83 -1.7 2,328,971 - BNILDI_11590 0.83 -0.7 2,329,029 - +0.2 2,329,044 + -2.4 2,329,097 - +0.2 2,329,103 + +0.3 2,329,119 + -0.3 2,329,119 + +0.2 2,329,120 - -2.7 2,329,120 - +0.2 2,329,120 - +0.1 2,329,122 + -2.0 2,329,122 + +0.2 2,329,122 + -0.5 2,329,122 + -0.3 2,329,123 - -1.4 2,329,269 - -1.4 2,329,288 - -0.0 2,329,331 - +1.0 2,329,331 - -0.1
Or see this region's nucleotide sequence