Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_11410 and BNILDI_11415 overlap by 32 nucleotides BNILDI_11415 and BNILDI_11420 are separated by 266 nucleotides BNILDI_11420 and BNILDI_11425 are separated by 315 nucleotides BNILDI_11425 and BNILDI_11430 overlap by 26 nucleotides
BNILDI_11410: BNILDI_11410 - relaxase, at 2,298,087 to 2,299,307
_11410
BNILDI_11415: BNILDI_11415 - hypothetical protein, at 2,299,276 to 2,299,704
_11415
BNILDI_11420: BNILDI_11420 - DUF2157 domain-containing protein, at 2,299,971 to 2,300,717
_11420
BNILDI_11425: BNILDI_11425 - hypothetical protein, at 2,301,033 to 2,301,191
_11425
BNILDI_11430: BNILDI_11430 - hypothetical protein, at 2,301,166 to 2,301,726
_11430
Position (kb)
2299
2300
2301 Strain fitness (log2 ratio)
-2
-1
0
1 at 2298.993 kb on + strand, within BNILDI_11410 at 2299.000 kb on + strand, within BNILDI_11410 at 2299.001 kb on - strand, within BNILDI_11410 at 2299.040 kb on + strand, within BNILDI_11410 at 2299.040 kb on + strand, within BNILDI_11410 at 2299.040 kb on + strand, within BNILDI_11410 at 2299.042 kb on + strand, within BNILDI_11410 at 2299.042 kb on + strand, within BNILDI_11410 at 2299.043 kb on - strand, within BNILDI_11410 at 2299.043 kb on - strand, within BNILDI_11410 at 2299.196 kb on + strand at 2299.196 kb on + strand at 2299.226 kb on + strand at 2299.227 kb on - strand at 2299.227 kb on - strand at 2299.227 kb on - strand at 2299.307 kb on + strand at 2299.321 kb on + strand, within BNILDI_11415 at 2299.387 kb on - strand, within BNILDI_11415 at 2299.402 kb on - strand, within BNILDI_11415 at 2299.413 kb on + strand, within BNILDI_11415 at 2299.414 kb on - strand, within BNILDI_11415 at 2299.420 kb on + strand, within BNILDI_11415 at 2299.420 kb on + strand, within BNILDI_11415 at 2299.420 kb on + strand, within BNILDI_11415 at 2299.420 kb on + strand, within BNILDI_11415 at 2300.373 kb on + strand, within BNILDI_11420 at 2300.374 kb on - strand, within BNILDI_11420 at 2300.415 kb on - strand, within BNILDI_11420 at 2300.589 kb on - strand, within BNILDI_11420 at 2300.967 kb on + strand at 2301.344 kb on - strand, within BNILDI_11430 at 2301.344 kb on - strand, within BNILDI_11430 at 2301.405 kb on + strand, within BNILDI_11430
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,298,993 + BNILDI_11410 0.74 -0.3 2,299,000 + BNILDI_11410 0.75 +0.2 2,299,001 - BNILDI_11410 0.75 +0.6 2,299,040 + BNILDI_11410 0.78 +0.2 2,299,040 + BNILDI_11410 0.78 -0.1 2,299,040 + BNILDI_11410 0.78 +0.2 2,299,042 + BNILDI_11410 0.78 -0.2 2,299,042 + BNILDI_11410 0.78 +1.7 2,299,043 - BNILDI_11410 0.78 +0.1 2,299,043 - BNILDI_11410 0.78 -0.1 2,299,196 + +0.2 2,299,196 + +0.1 2,299,226 + -1.0 2,299,227 - -0.0 2,299,227 - +0.7 2,299,227 - +0.2 2,299,307 + -0.2 2,299,321 + BNILDI_11415 0.10 -1.9 2,299,387 - BNILDI_11415 0.26 -0.6 2,299,402 - BNILDI_11415 0.29 -0.7 2,299,413 + BNILDI_11415 0.32 -0.1 2,299,414 - BNILDI_11415 0.32 -1.0 2,299,420 + BNILDI_11415 0.34 +0.3 2,299,420 + BNILDI_11415 0.34 -0.1 2,299,420 + BNILDI_11415 0.34 -0.1 2,299,420 + BNILDI_11415 0.34 +0.5 2,300,373 + BNILDI_11420 0.54 +0.1 2,300,374 - BNILDI_11420 0.54 +0.5 2,300,415 - BNILDI_11420 0.59 +0.7 2,300,589 - BNILDI_11420 0.83 +0.3 2,300,967 + +0.4 2,301,344 - BNILDI_11430 0.32 +0.5 2,301,344 - BNILDI_11430 0.32 -0.4 2,301,405 + BNILDI_11430 0.43 -1.2
Or see this region's nucleotide sequence