Strain Fitness in Escherichia coli ECRC62 around BNILDI_10965

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypB and hypA are separated by 3 nucleotideshypA and hycA are separated by 211 nucleotideshycA and hycB are separated by 124 nucleotideshycB and hycC overlap by 4 nucleotides BNILDI_10955: hypB - hydrogenase nickel incorporation protein HypB, at 2,214,276 to 2,215,148 hypB BNILDI_10960: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,215,152 to 2,215,502 hypA BNILDI_10965: hycA - formate hydrogenlyase regulator HycA, at 2,215,714 to 2,216,175 hycA BNILDI_10970: hycB - formate hydrogenlyase subunit HycB, at 2,216,300 to 2,216,911 hycB BNILDI_10975: hycC - formate hydrogenlyase subunit 3, at 2,216,908 to 2,218,734 hycC Position (kb) 2215 2216 2217Strain fitness (log2 ratio) -2 -1 0 1 2at 2214.741 kb on - strand, within hypBat 2215.111 kb on + strandat 2215.142 kb on + strandat 2215.142 kb on + strandat 2215.143 kb on - strandat 2215.143 kb on - strandat 2215.164 kb on + strandat 2215.165 kb on - strandat 2215.186 kb on - strandat 2215.209 kb on + strand, within hypAat 2215.210 kb on - strand, within hypAat 2215.267 kb on + strand, within hypAat 2215.267 kb on + strand, within hypAat 2215.268 kb on - strand, within hypAat 2215.268 kb on - strand, within hypAat 2215.268 kb on - strand, within hypAat 2215.426 kb on - strand, within hypAat 2215.426 kb on - strand, within hypAat 2215.515 kb on + strandat 2215.520 kb on - strandat 2215.520 kb on - strandat 2215.720 kb on - strandat 2215.720 kb on - strandat 2215.773 kb on - strand, within hycAat 2215.793 kb on - strand, within hycAat 2215.948 kb on + strand, within hycAat 2215.951 kb on + strand, within hycAat 2215.951 kb on + strand, within hycAat 2215.951 kb on + strand, within hycAat 2216.069 kb on - strand, within hycAat 2216.103 kb on - strand, within hycAat 2216.103 kb on - strand, within hycAat 2216.103 kb on - strand, within hycAat 2216.104 kb on + strand, within hycAat 2216.104 kb on + strand, within hycAat 2216.151 kb on - strandat 2216.313 kb on + strandat 2216.314 kb on - strandat 2216.422 kb on - strand, within hycBat 2216.422 kb on - strand, within hycBat 2216.547 kb on + strand, within hycBat 2216.851 kb on + strandat 2216.905 kb on + strandat 2216.906 kb on - strandat 2217.041 kb on - strandat 2217.042 kb on + strandat 2217.108 kb on - strand, within hycCat 2217.113 kb on - strand, within hycCat 2217.113 kb on - strand, within hycCat 2217.113 kb on - strand, within hycCat 2217.135 kb on + strand, within hycC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,214,741 - hypB BNILDI_10955 0.53 +1.2
2,215,111 + +0.2
2,215,142 + -0.3
2,215,142 + -0.3
2,215,143 - +0.1
2,215,143 - -1.0
2,215,164 + +0.5
2,215,165 - +0.3
2,215,186 - -0.1
2,215,209 + hypA BNILDI_10960 0.16 -1.0
2,215,210 - hypA BNILDI_10960 0.17 -0.3
2,215,267 + hypA BNILDI_10960 0.33 +0.2
2,215,267 + hypA BNILDI_10960 0.33 +1.9
2,215,268 - hypA BNILDI_10960 0.33 -0.6
2,215,268 - hypA BNILDI_10960 0.33 -0.0
2,215,268 - hypA BNILDI_10960 0.33 +0.9
2,215,426 - hypA BNILDI_10960 0.78 +0.7
2,215,426 - hypA BNILDI_10960 0.78 -0.3
2,215,515 + -1.6
2,215,520 - -0.2
2,215,520 - -0.1
2,215,720 - -0.1
2,215,720 - +1.0
2,215,773 - hycA BNILDI_10965 0.13 -0.6
2,215,793 - hycA BNILDI_10965 0.17 -0.2
2,215,948 + hycA BNILDI_10965 0.51 +0.4
2,215,951 + hycA BNILDI_10965 0.51 -0.9
2,215,951 + hycA BNILDI_10965 0.51 +0.2
2,215,951 + hycA BNILDI_10965 0.51 -1.6
2,216,069 - hycA BNILDI_10965 0.77 +1.5
2,216,103 - hycA BNILDI_10965 0.84 -1.4
2,216,103 - hycA BNILDI_10965 0.84 +0.5
2,216,103 - hycA BNILDI_10965 0.84 -0.1
2,216,104 + hycA BNILDI_10965 0.84 +0.4
2,216,104 + hycA BNILDI_10965 0.84 +0.4
2,216,151 - +0.0
2,216,313 + -0.0
2,216,314 - +0.2
2,216,422 - hycB BNILDI_10970 0.20 +0.9
2,216,422 - hycB BNILDI_10970 0.20 +0.2
2,216,547 + hycB BNILDI_10970 0.40 +0.1
2,216,851 + -0.4
2,216,905 + +0.3
2,216,906 - -2.5
2,217,041 - +0.9
2,217,042 + -0.9
2,217,108 - hycC BNILDI_10975 0.11 +0.4
2,217,113 - hycC BNILDI_10975 0.11 +0.4
2,217,113 - hycC BNILDI_10975 0.11 +0.9
2,217,113 - hycC BNILDI_10975 0.11 -1.2
2,217,135 + hycC BNILDI_10975 0.12 -1.3

Or see this region's nucleotide sequence