Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypB and hypA are separated by 3 nucleotides hypA and hycA are separated by 211 nucleotides hycA and hycB are separated by 124 nucleotides hycB and hycC overlap by 4 nucleotides
BNILDI_10955: hypB - hydrogenase nickel incorporation protein HypB, at 2,214,276 to 2,215,148
hypB
BNILDI_10960: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,215,152 to 2,215,502
hypA
BNILDI_10965: hycA - formate hydrogenlyase regulator HycA, at 2,215,714 to 2,216,175
hycA
BNILDI_10970: hycB - formate hydrogenlyase subunit HycB, at 2,216,300 to 2,216,911
hycB
BNILDI_10975: hycC - formate hydrogenlyase subunit 3, at 2,216,908 to 2,218,734
hycC
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2214.741 kb on - strand, within hypB at 2215.111 kb on + strand at 2215.142 kb on + strand at 2215.142 kb on + strand at 2215.143 kb on - strand at 2215.143 kb on - strand at 2215.164 kb on + strand at 2215.165 kb on - strand at 2215.186 kb on - strand at 2215.209 kb on + strand, within hypA at 2215.210 kb on - strand, within hypA at 2215.267 kb on + strand, within hypA at 2215.267 kb on + strand, within hypA at 2215.268 kb on - strand, within hypA at 2215.268 kb on - strand, within hypA at 2215.268 kb on - strand, within hypA at 2215.426 kb on - strand, within hypA at 2215.426 kb on - strand, within hypA at 2215.515 kb on + strand at 2215.520 kb on - strand at 2215.520 kb on - strand at 2215.720 kb on - strand at 2215.720 kb on - strand at 2215.773 kb on - strand, within hycA at 2215.793 kb on - strand, within hycA at 2215.948 kb on + strand, within hycA at 2215.951 kb on + strand, within hycA at 2215.951 kb on + strand, within hycA at 2215.951 kb on + strand, within hycA at 2216.069 kb on - strand, within hycA at 2216.103 kb on - strand, within hycA at 2216.103 kb on - strand, within hycA at 2216.103 kb on - strand, within hycA at 2216.104 kb on + strand, within hycA at 2216.104 kb on + strand, within hycA at 2216.151 kb on - strand at 2216.313 kb on + strand at 2216.314 kb on - strand at 2216.422 kb on - strand, within hycB at 2216.422 kb on - strand, within hycB at 2216.547 kb on + strand, within hycB at 2216.851 kb on + strand at 2216.905 kb on + strand at 2216.906 kb on - strand at 2217.041 kb on - strand at 2217.042 kb on + strand at 2217.108 kb on - strand, within hycC at 2217.113 kb on - strand, within hycC at 2217.113 kb on - strand, within hycC at 2217.113 kb on - strand, within hycC at 2217.135 kb on + strand, within hycC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,214,741 - hypB BNILDI_10955 0.53 +1.2 2,215,111 + +0.2 2,215,142 + -0.3 2,215,142 + -0.3 2,215,143 - +0.1 2,215,143 - -1.0 2,215,164 + +0.5 2,215,165 - +0.3 2,215,186 - -0.1 2,215,209 + hypA BNILDI_10960 0.16 -1.0 2,215,210 - hypA BNILDI_10960 0.17 -0.3 2,215,267 + hypA BNILDI_10960 0.33 +0.2 2,215,267 + hypA BNILDI_10960 0.33 +1.9 2,215,268 - hypA BNILDI_10960 0.33 -0.6 2,215,268 - hypA BNILDI_10960 0.33 -0.0 2,215,268 - hypA BNILDI_10960 0.33 +0.9 2,215,426 - hypA BNILDI_10960 0.78 +0.7 2,215,426 - hypA BNILDI_10960 0.78 -0.3 2,215,515 + -1.6 2,215,520 - -0.2 2,215,520 - -0.1 2,215,720 - -0.1 2,215,720 - +1.0 2,215,773 - hycA BNILDI_10965 0.13 -0.6 2,215,793 - hycA BNILDI_10965 0.17 -0.2 2,215,948 + hycA BNILDI_10965 0.51 +0.4 2,215,951 + hycA BNILDI_10965 0.51 -0.9 2,215,951 + hycA BNILDI_10965 0.51 +0.2 2,215,951 + hycA BNILDI_10965 0.51 -1.6 2,216,069 - hycA BNILDI_10965 0.77 +1.5 2,216,103 - hycA BNILDI_10965 0.84 -1.4 2,216,103 - hycA BNILDI_10965 0.84 +0.5 2,216,103 - hycA BNILDI_10965 0.84 -0.1 2,216,104 + hycA BNILDI_10965 0.84 +0.4 2,216,104 + hycA BNILDI_10965 0.84 +0.4 2,216,151 - +0.0 2,216,313 + -0.0 2,216,314 - +0.2 2,216,422 - hycB BNILDI_10970 0.20 +0.9 2,216,422 - hycB BNILDI_10970 0.20 +0.2 2,216,547 + hycB BNILDI_10970 0.40 +0.1 2,216,851 + -0.4 2,216,905 + +0.3 2,216,906 - -2.5 2,217,041 - +0.9 2,217,042 + -0.9 2,217,108 - hycC BNILDI_10975 0.11 +0.4 2,217,113 - hycC BNILDI_10975 0.11 +0.4 2,217,113 - hycC BNILDI_10975 0.11 +0.9 2,217,113 - hycC BNILDI_10975 0.11 -1.2 2,217,135 + hycC BNILDI_10975 0.12 -1.3
Or see this region's nucleotide sequence