Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xdhA and actS are separated by 414 nucleotides actS and Gly_trna are separated by 78 nucleotides Gly_trna and BNILDI_10180 are separated by 504 nucleotides
BNILDI_10165: xdhA - xanthine dehydrogenase molybdenum-binding subunit XdhA, at 2,048,938 to 2,051,235
xdhA
BNILDI_10170: actS - amidase activator ActS, at 2,051,650 to 2,052,405
actS
BNILDI_10175: Gly_trna - tRNA-Gly(ccc), at 2,052,484 to 2,052,557
_trna
BNILDI_10180: BNILDI_10180 - hypothetical protein, at 2,053,062 to 2,053,637
_10180
Position (kb)
2051
2052
2053 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2050.688 kb on - strand, within xdhA at 2050.726 kb on - strand, within xdhA at 2050.930 kb on + strand, within xdhA at 2050.931 kb on - strand, within xdhA at 2051.004 kb on + strand, within xdhA at 2051.074 kb on - strand at 2051.103 kb on + strand at 2051.103 kb on + strand at 2051.107 kb on + strand at 2051.107 kb on + strand at 2051.107 kb on + strand at 2051.108 kb on - strand at 2051.108 kb on - strand at 2051.111 kb on + strand at 2051.111 kb on + strand at 2051.136 kb on + strand at 2051.137 kb on - strand at 2051.151 kb on + strand at 2051.151 kb on + strand at 2051.151 kb on + strand at 2051.153 kb on + strand at 2051.154 kb on - strand at 2051.176 kb on - strand at 2051.334 kb on - strand at 2051.365 kb on + strand at 2051.366 kb on - strand at 2051.401 kb on + strand at 2051.401 kb on + strand at 2051.402 kb on - strand at 2051.402 kb on - strand at 2051.402 kb on - strand at 2051.402 kb on - strand at 2051.406 kb on - strand at 2051.406 kb on - strand at 2051.406 kb on - strand at 2051.406 kb on - strand at 2051.412 kb on - strand at 2051.412 kb on - strand at 2051.437 kb on + strand at 2051.438 kb on - strand at 2051.448 kb on + strand at 2051.449 kb on - strand at 2051.471 kb on - strand at 2051.471 kb on - strand at 2051.535 kb on - strand at 2051.758 kb on + strand, within actS at 2051.773 kb on + strand, within actS at 2051.774 kb on - strand, within actS at 2051.774 kb on - strand, within actS at 2051.794 kb on - strand, within actS at 2051.798 kb on + strand, within actS at 2051.798 kb on + strand, within actS at 2051.799 kb on - strand, within actS at 2051.799 kb on - strand, within actS at 2051.799 kb on - strand, within actS at 2051.799 kb on - strand, within actS at 2051.805 kb on + strand, within actS at 2051.805 kb on + strand, within actS at 2051.806 kb on - strand, within actS at 2051.806 kb on - strand, within actS at 2051.869 kb on + strand, within actS at 2051.870 kb on - strand, within actS at 2051.916 kb on + strand, within actS at 2051.923 kb on - strand, within actS at 2051.926 kb on - strand, within actS at 2052.051 kb on + strand, within actS at 2052.051 kb on + strand, within actS at 2052.052 kb on - strand, within actS at 2052.085 kb on - strand, within actS at 2052.096 kb on + strand, within actS at 2052.097 kb on - strand, within actS at 2052.121 kb on - strand, within actS at 2052.151 kb on + strand, within actS at 2052.151 kb on + strand, within actS at 2052.151 kb on + strand, within actS at 2052.151 kb on + strand, within actS at 2052.151 kb on + strand, within actS at 2052.152 kb on - strand, within actS at 2052.152 kb on - strand, within actS at 2052.152 kb on - strand, within actS at 2052.152 kb on - strand, within actS at 2052.152 kb on - strand, within actS at 2052.180 kb on + strand, within actS at 2052.211 kb on + strand, within actS at 2052.252 kb on + strand, within actS at 2052.370 kb on + strand at 2052.371 kb on - strand at 2052.371 kb on - strand at 2052.455 kb on - strand at 2052.464 kb on - strand at 2052.464 kb on - strand at 2052.502 kb on + strand, within Gly_trna at 2052.525 kb on + strand, within Gly_trna at 2052.525 kb on + strand, within Gly_trna at 2052.526 kb on - strand, within Gly_trna at 2052.664 kb on + strand at 2052.701 kb on + strand at 2052.777 kb on + strand at 2052.778 kb on - strand at 2052.836 kb on - strand at 2052.971 kb on + strand at 2052.971 kb on + strand at 2052.972 kb on - strand at 2053.045 kb on + strand at 2053.046 kb on - strand at 2053.082 kb on + strand at 2053.164 kb on + strand, within BNILDI_10180 at 2053.164 kb on + strand, within BNILDI_10180 at 2053.164 kb on + strand, within BNILDI_10180 at 2053.202 kb on + strand, within BNILDI_10180 at 2053.203 kb on - strand, within BNILDI_10180 at 2053.343 kb on + strand, within BNILDI_10180 at 2053.356 kb on - strand, within BNILDI_10180 at 2053.369 kb on + strand, within BNILDI_10180 at 2053.404 kb on - strand, within BNILDI_10180
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,050,688 - xdhA BNILDI_10165 0.76 +1.0 2,050,726 - xdhA BNILDI_10165 0.78 +0.3 2,050,930 + xdhA BNILDI_10165 0.87 +0.6 2,050,931 - xdhA BNILDI_10165 0.87 -0.9 2,051,004 + xdhA BNILDI_10165 0.90 +0.5 2,051,074 - +0.2 2,051,103 + +0.9 2,051,103 + +0.2 2,051,107 + -0.2 2,051,107 + +0.5 2,051,107 + -0.0 2,051,108 - -0.7 2,051,108 - -0.3 2,051,111 + +0.5 2,051,111 + +0.3 2,051,136 + +1.1 2,051,137 - +0.4 2,051,151 + -1.6 2,051,151 + +0.1 2,051,151 + -1.4 2,051,153 + +0.1 2,051,154 - +1.8 2,051,176 - -0.3 2,051,334 - +0.5 2,051,365 + -0.4 2,051,366 - -0.1 2,051,401 + -1.1 2,051,401 + -1.5 2,051,402 - +0.6 2,051,402 - -0.4 2,051,402 - -0.2 2,051,402 - -2.1 2,051,406 - -0.8 2,051,406 - +0.2 2,051,406 - +0.5 2,051,406 - -1.0 2,051,412 - -0.7 2,051,412 - +0.2 2,051,437 + -0.4 2,051,438 - -0.5 2,051,448 + -0.5 2,051,449 - +0.2 2,051,471 - -0.5 2,051,471 - -1.8 2,051,535 - +1.3 2,051,758 + actS BNILDI_10170 0.14 +1.5 2,051,773 + actS BNILDI_10170 0.16 +0.1 2,051,774 - actS BNILDI_10170 0.16 -0.3 2,051,774 - actS BNILDI_10170 0.16 +0.0 2,051,794 - actS BNILDI_10170 0.19 -0.1 2,051,798 + actS BNILDI_10170 0.20 +0.1 2,051,798 + actS BNILDI_10170 0.20 -0.3 2,051,799 - actS BNILDI_10170 0.20 +0.0 2,051,799 - actS BNILDI_10170 0.20 -1.1 2,051,799 - actS BNILDI_10170 0.20 +0.0 2,051,799 - actS BNILDI_10170 0.20 +0.6 2,051,805 + actS BNILDI_10170 0.21 +0.2 2,051,805 + actS BNILDI_10170 0.21 +0.5 2,051,806 - actS BNILDI_10170 0.21 +2.1 2,051,806 - actS BNILDI_10170 0.21 -1.2 2,051,869 + actS BNILDI_10170 0.29 +0.2 2,051,870 - actS BNILDI_10170 0.29 +0.3 2,051,916 + actS BNILDI_10170 0.35 -0.0 2,051,923 - actS BNILDI_10170 0.36 +0.0 2,051,926 - actS BNILDI_10170 0.37 -0.3 2,052,051 + actS BNILDI_10170 0.53 +0.4 2,052,051 + actS BNILDI_10170 0.53 +1.8 2,052,052 - actS BNILDI_10170 0.53 +0.2 2,052,085 - actS BNILDI_10170 0.58 -0.4 2,052,096 + actS BNILDI_10170 0.59 +0.4 2,052,097 - actS BNILDI_10170 0.59 +1.2 2,052,121 - actS BNILDI_10170 0.62 +0.9 2,052,151 + actS BNILDI_10170 0.66 +0.6 2,052,151 + actS BNILDI_10170 0.66 +0.2 2,052,151 + actS BNILDI_10170 0.66 +0.3 2,052,151 + actS BNILDI_10170 0.66 +0.6 2,052,151 + actS BNILDI_10170 0.66 +0.7 2,052,152 - actS BNILDI_10170 0.66 +0.0 2,052,152 - actS BNILDI_10170 0.66 +0.4 2,052,152 - actS BNILDI_10170 0.66 +0.2 2,052,152 - actS BNILDI_10170 0.66 -0.0 2,052,152 - actS BNILDI_10170 0.66 -0.1 2,052,180 + actS BNILDI_10170 0.70 +0.5 2,052,211 + actS BNILDI_10170 0.74 -1.1 2,052,252 + actS BNILDI_10170 0.80 +0.6 2,052,370 + -0.5 2,052,371 - +1.0 2,052,371 - +0.1 2,052,455 - -0.2 2,052,464 - -0.9 2,052,464 - +0.4 2,052,502 + Gly_trna BNILDI_10175 0.24 +0.0 2,052,525 + Gly_trna BNILDI_10175 0.55 +0.1 2,052,525 + Gly_trna BNILDI_10175 0.55 -1.4 2,052,526 - Gly_trna BNILDI_10175 0.57 -0.5 2,052,664 + -0.1 2,052,701 + +3.9 2,052,777 + -1.1 2,052,778 - -0.5 2,052,836 - +0.0 2,052,971 + -0.6 2,052,971 + +0.5 2,052,972 - +0.4 2,053,045 + -0.0 2,053,046 - +0.7 2,053,082 + +1.3 2,053,164 + BNILDI_10180 0.18 -1.5 2,053,164 + BNILDI_10180 0.18 +0.1 2,053,164 + BNILDI_10180 0.18 +2.1 2,053,202 + BNILDI_10180 0.24 -0.0 2,053,203 - BNILDI_10180 0.24 +0.3 2,053,343 + BNILDI_10180 0.49 -0.3 2,053,356 - BNILDI_10180 0.51 -0.1 2,053,369 + BNILDI_10180 0.53 +1.6 2,053,404 - BNILDI_10180 0.59 -0.5
Or see this region's nucleotide sequence