Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and dsbC are separated by 24 nucleotides dsbC and recJ are separated by 5 nucleotides recJ and prfB are separated by 307 nucleotides
BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875
xerD
BNILDI_10030: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 2,012,900 to 2,013,610
dsbC
BNILDI_10035: recJ - single-stranded-DNA-specific exonuclease RecJ, at 2,013,616 to 2,015,349
recJ
BNILDI_10040: prfB - peptide chain release factor 2, at 2,015,657 to 2,016,538
prfB
Position (kb)
2013
2014
2015
2016 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2012.739 kb on + strand, within xerD at 2012.914 kb on + strand at 2012.914 kb on + strand at 2012.915 kb on - strand at 2012.915 kb on - strand at 2013.071 kb on + strand, within dsbC at 2013.072 kb on - strand, within dsbC at 2013.072 kb on - strand, within dsbC at 2013.094 kb on + strand, within dsbC at 2013.095 kb on - strand, within dsbC at 2013.095 kb on - strand, within dsbC at 2013.113 kb on + strand, within dsbC at 2013.113 kb on + strand, within dsbC at 2013.114 kb on - strand, within dsbC at 2013.244 kb on + strand, within dsbC at 2013.245 kb on - strand, within dsbC at 2013.247 kb on + strand, within dsbC at 2013.248 kb on - strand, within dsbC at 2013.292 kb on + strand, within dsbC at 2013.373 kb on + strand, within dsbC at 2013.424 kb on + strand, within dsbC at 2013.425 kb on - strand, within dsbC at 2013.457 kb on + strand, within dsbC at 2013.630 kb on - strand at 2013.757 kb on - strand at 2013.757 kb on - strand at 2013.808 kb on + strand, within recJ at 2013.808 kb on + strand, within recJ at 2013.884 kb on + strand, within recJ at 2013.885 kb on - strand, within recJ at 2013.928 kb on + strand, within recJ at 2013.935 kb on + strand, within recJ at 2013.966 kb on - strand, within recJ at 2014.011 kb on - strand, within recJ at 2014.032 kb on + strand, within recJ at 2014.341 kb on + strand, within recJ at 2014.344 kb on + strand, within recJ at 2014.345 kb on - strand, within recJ at 2014.407 kb on + strand, within recJ at 2014.407 kb on + strand, within recJ at 2014.408 kb on - strand, within recJ at 2014.408 kb on - strand, within recJ at 2014.512 kb on + strand, within recJ at 2014.589 kb on + strand, within recJ at 2014.696 kb on + strand, within recJ at 2014.697 kb on - strand, within recJ at 2014.870 kb on + strand, within recJ at 2014.884 kb on - strand, within recJ at 2015.211 kb on + strand at 2015.220 kb on + strand at 2015.220 kb on + strand at 2015.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,012,739 + xerD BNILDI_10025 0.85 -2.6 2,012,914 + -0.6 2,012,914 + -0.1 2,012,915 - +0.4 2,012,915 - +1.5 2,013,071 + dsbC BNILDI_10030 0.24 +0.8 2,013,072 - dsbC BNILDI_10030 0.24 +0.3 2,013,072 - dsbC BNILDI_10030 0.24 -1.5 2,013,094 + dsbC BNILDI_10030 0.27 -0.5 2,013,095 - dsbC BNILDI_10030 0.27 -0.0 2,013,095 - dsbC BNILDI_10030 0.27 -1.5 2,013,113 + dsbC BNILDI_10030 0.30 -0.4 2,013,113 + dsbC BNILDI_10030 0.30 +0.6 2,013,114 - dsbC BNILDI_10030 0.30 -0.7 2,013,244 + dsbC BNILDI_10030 0.48 -0.1 2,013,245 - dsbC BNILDI_10030 0.49 -3.2 2,013,247 + dsbC BNILDI_10030 0.49 -0.1 2,013,248 - dsbC BNILDI_10030 0.49 -0.1 2,013,292 + dsbC BNILDI_10030 0.55 +1.6 2,013,373 + dsbC BNILDI_10030 0.67 -0.1 2,013,424 + dsbC BNILDI_10030 0.74 +0.6 2,013,425 - dsbC BNILDI_10030 0.74 -0.7 2,013,457 + dsbC BNILDI_10030 0.78 +1.0 2,013,630 - -1.1 2,013,757 - +0.4 2,013,757 - -0.1 2,013,808 + recJ BNILDI_10035 0.11 -0.4 2,013,808 + recJ BNILDI_10035 0.11 -0.8 2,013,884 + recJ BNILDI_10035 0.15 -0.4 2,013,885 - recJ BNILDI_10035 0.16 +0.1 2,013,928 + recJ BNILDI_10035 0.18 -0.0 2,013,935 + recJ BNILDI_10035 0.18 -0.1 2,013,966 - recJ BNILDI_10035 0.20 -1.0 2,014,011 - recJ BNILDI_10035 0.23 -1.4 2,014,032 + recJ BNILDI_10035 0.24 +0.2 2,014,341 + recJ BNILDI_10035 0.42 +0.0 2,014,344 + recJ BNILDI_10035 0.42 -0.0 2,014,345 - recJ BNILDI_10035 0.42 +0.1 2,014,407 + recJ BNILDI_10035 0.46 -0.3 2,014,407 + recJ BNILDI_10035 0.46 +0.6 2,014,408 - recJ BNILDI_10035 0.46 -2.5 2,014,408 - recJ BNILDI_10035 0.46 -1.0 2,014,512 + recJ BNILDI_10035 0.52 -1.7 2,014,589 + recJ BNILDI_10035 0.56 +0.2 2,014,696 + recJ BNILDI_10035 0.62 -1.2 2,014,697 - recJ BNILDI_10035 0.62 +0.1 2,014,870 + recJ BNILDI_10035 0.72 -0.3 2,014,884 - recJ BNILDI_10035 0.73 +0.5 2,015,211 + -0.6 2,015,220 + +0.9 2,015,220 + -1.5 2,015,276 - -0.4
Or see this region's nucleotide sequence