Strain Fitness in Escherichia coli ECRC62 around BNILDI_10035

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntxerD and dsbC are separated by 24 nucleotidesdsbC and recJ are separated by 5 nucleotidesrecJ and prfB are separated by 307 nucleotides BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875 xerD BNILDI_10030: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 2,012,900 to 2,013,610 dsbC BNILDI_10035: recJ - single-stranded-DNA-specific exonuclease RecJ, at 2,013,616 to 2,015,349 recJ BNILDI_10040: prfB - peptide chain release factor 2, at 2,015,657 to 2,016,538 prfB Position (kb) 2013 2014 2015 2016Strain fitness (log2 ratio) -3 -2 -1 0 1at 2012.739 kb on + strand, within xerDat 2012.914 kb on + strandat 2012.914 kb on + strandat 2012.915 kb on - strandat 2012.915 kb on - strandat 2013.071 kb on + strand, within dsbCat 2013.072 kb on - strand, within dsbCat 2013.072 kb on - strand, within dsbCat 2013.094 kb on + strand, within dsbCat 2013.095 kb on - strand, within dsbCat 2013.095 kb on - strand, within dsbCat 2013.113 kb on + strand, within dsbCat 2013.113 kb on + strand, within dsbCat 2013.114 kb on - strand, within dsbCat 2013.244 kb on + strand, within dsbCat 2013.245 kb on - strand, within dsbCat 2013.247 kb on + strand, within dsbCat 2013.248 kb on - strand, within dsbCat 2013.292 kb on + strand, within dsbCat 2013.373 kb on + strand, within dsbCat 2013.424 kb on + strand, within dsbCat 2013.425 kb on - strand, within dsbCat 2013.457 kb on + strand, within dsbCat 2013.630 kb on - strandat 2013.757 kb on - strandat 2013.757 kb on - strandat 2013.808 kb on + strand, within recJat 2013.808 kb on + strand, within recJat 2013.884 kb on + strand, within recJat 2013.885 kb on - strand, within recJat 2013.928 kb on + strand, within recJat 2013.935 kb on + strand, within recJat 2013.966 kb on - strand, within recJat 2014.011 kb on - strand, within recJat 2014.032 kb on + strand, within recJat 2014.341 kb on + strand, within recJat 2014.344 kb on + strand, within recJat 2014.345 kb on - strand, within recJat 2014.407 kb on + strand, within recJat 2014.407 kb on + strand, within recJat 2014.408 kb on - strand, within recJat 2014.408 kb on - strand, within recJat 2014.512 kb on + strand, within recJat 2014.589 kb on + strand, within recJat 2014.696 kb on + strand, within recJat 2014.697 kb on - strand, within recJat 2014.870 kb on + strand, within recJat 2014.884 kb on - strand, within recJat 2015.211 kb on + strandat 2015.220 kb on + strandat 2015.220 kb on + strandat 2015.276 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,012,739 + xerD BNILDI_10025 0.85 -2.6
2,012,914 + -0.6
2,012,914 + -0.1
2,012,915 - +0.4
2,012,915 - +1.5
2,013,071 + dsbC BNILDI_10030 0.24 +0.8
2,013,072 - dsbC BNILDI_10030 0.24 +0.3
2,013,072 - dsbC BNILDI_10030 0.24 -1.5
2,013,094 + dsbC BNILDI_10030 0.27 -0.5
2,013,095 - dsbC BNILDI_10030 0.27 -0.0
2,013,095 - dsbC BNILDI_10030 0.27 -1.5
2,013,113 + dsbC BNILDI_10030 0.30 -0.4
2,013,113 + dsbC BNILDI_10030 0.30 +0.6
2,013,114 - dsbC BNILDI_10030 0.30 -0.7
2,013,244 + dsbC BNILDI_10030 0.48 -0.1
2,013,245 - dsbC BNILDI_10030 0.49 -3.2
2,013,247 + dsbC BNILDI_10030 0.49 -0.1
2,013,248 - dsbC BNILDI_10030 0.49 -0.1
2,013,292 + dsbC BNILDI_10030 0.55 +1.6
2,013,373 + dsbC BNILDI_10030 0.67 -0.1
2,013,424 + dsbC BNILDI_10030 0.74 +0.6
2,013,425 - dsbC BNILDI_10030 0.74 -0.7
2,013,457 + dsbC BNILDI_10030 0.78 +1.0
2,013,630 - -1.1
2,013,757 - +0.4
2,013,757 - -0.1
2,013,808 + recJ BNILDI_10035 0.11 -0.4
2,013,808 + recJ BNILDI_10035 0.11 -0.8
2,013,884 + recJ BNILDI_10035 0.15 -0.4
2,013,885 - recJ BNILDI_10035 0.16 +0.1
2,013,928 + recJ BNILDI_10035 0.18 -0.0
2,013,935 + recJ BNILDI_10035 0.18 -0.1
2,013,966 - recJ BNILDI_10035 0.20 -1.0
2,014,011 - recJ BNILDI_10035 0.23 -1.4
2,014,032 + recJ BNILDI_10035 0.24 +0.2
2,014,341 + recJ BNILDI_10035 0.42 +0.0
2,014,344 + recJ BNILDI_10035 0.42 -0.0
2,014,345 - recJ BNILDI_10035 0.42 +0.1
2,014,407 + recJ BNILDI_10035 0.46 -0.3
2,014,407 + recJ BNILDI_10035 0.46 +0.6
2,014,408 - recJ BNILDI_10035 0.46 -2.5
2,014,408 - recJ BNILDI_10035 0.46 -1.0
2,014,512 + recJ BNILDI_10035 0.52 -1.7
2,014,589 + recJ BNILDI_10035 0.56 +0.2
2,014,696 + recJ BNILDI_10035 0.62 -1.2
2,014,697 - recJ BNILDI_10035 0.62 +0.1
2,014,870 + recJ BNILDI_10035 0.72 -0.3
2,014,884 - recJ BNILDI_10035 0.73 +0.5
2,015,211 + -0.6
2,015,220 + +0.9
2,015,220 + -1.5
2,015,276 - -0.4

Or see this region's nucleotide sequence