Strain Fitness in Escherichia coli ECRC62 around BNILDI_09815

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcoaA and cysA overlap by 29 nucleotidescysA and ecfA2 overlap by 7 nucleotidesecfA2 and BNILDI_09820 overlap by 13 nucleotidesBNILDI_09820 and BNILDI_09825 are separated by 0 nucleotides BNILDI_09805: coaA - nucleoside/nucleotide kinase family protein, at 1,973,799 to 1,974,512 coaA BNILDI_09810: cysA - ABC transporter ATP-binding protein, at 1,974,484 to 1,975,161 cysA BNILDI_09815: ecfA2 - cobalt ABC transporter ATP-binding protein, at 1,975,155 to 1,975,832 ecfA2 BNILDI_09820: BNILDI_09820 - cobalt ABC transporter permease, at 1,975,820 to 1,976,527 _09820 BNILDI_09825: BNILDI_09825 - ABC-type transport system, at 1,976,528 to 1,977,106 _09825 Position (kb) 1975 1976Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1974.167 kb on + strand, within coaAat 1974.168 kb on - strand, within coaAat 1974.168 kb on - strand, within coaAat 1974.405 kb on + strand, within coaAat 1974.568 kb on - strand, within cysAat 1974.814 kb on - strand, within cysAat 1975.278 kb on + strand, within ecfA2at 1975.278 kb on + strand, within ecfA2at 1975.541 kb on + strand, within ecfA2at 1975.653 kb on + strand, within ecfA2at 1975.759 kb on + strand, within ecfA2at 1975.760 kb on - strand, within ecfA2at 1975.821 kb on + strandat 1975.826 kb on + strandat 1975.826 kb on + strandat 1975.892 kb on + strand, within BNILDI_09820at 1975.987 kb on + strand, within BNILDI_09820at 1976.111 kb on + strand, within BNILDI_09820at 1976.112 kb on - strand, within BNILDI_09820at 1976.148 kb on + strand, within BNILDI_09820at 1976.148 kb on + strand, within BNILDI_09820at 1976.149 kb on - strand, within BNILDI_09820at 1976.212 kb on - strand, within BNILDI_09820at 1976.266 kb on + strand, within BNILDI_09820at 1976.270 kb on - strand, within BNILDI_09820at 1976.270 kb on - strand, within BNILDI_09820at 1976.270 kb on - strand, within BNILDI_09820at 1976.309 kb on + strand, within BNILDI_09820at 1976.382 kb on + strand, within BNILDI_09820at 1976.383 kb on - strand, within BNILDI_09820at 1976.383 kb on - strand, within BNILDI_09820at 1976.383 kb on - strand, within BNILDI_09820at 1976.442 kb on + strand, within BNILDI_09820at 1976.779 kb on + strand, within BNILDI_09825at 1976.779 kb on + strand, within BNILDI_09825at 1976.779 kb on + strand, within BNILDI_09825at 1976.782 kb on + strand, within BNILDI_09825

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,974,167 + coaA BNILDI_09805 0.52 -0.5
1,974,168 - coaA BNILDI_09805 0.52 +0.2
1,974,168 - coaA BNILDI_09805 0.52 -0.8
1,974,405 + coaA BNILDI_09805 0.85 -0.4
1,974,568 - cysA BNILDI_09810 0.12 -1.9
1,974,814 - cysA BNILDI_09810 0.49 -0.1
1,975,278 + ecfA2 BNILDI_09815 0.18 -1.3
1,975,278 + ecfA2 BNILDI_09815 0.18 +0.1
1,975,541 + ecfA2 BNILDI_09815 0.57 -1.5
1,975,653 + ecfA2 BNILDI_09815 0.73 -0.2
1,975,759 + ecfA2 BNILDI_09815 0.89 -0.4
1,975,760 - ecfA2 BNILDI_09815 0.89 +0.3
1,975,821 + +0.1
1,975,826 + -1.0
1,975,826 + -0.0
1,975,892 + BNILDI_09820 0.10 +1.9
1,975,987 + BNILDI_09820 0.24 +0.0
1,976,111 + BNILDI_09820 0.41 +0.6
1,976,112 - BNILDI_09820 0.41 -0.3
1,976,148 + BNILDI_09820 0.46 +0.2
1,976,148 + BNILDI_09820 0.46 +0.4
1,976,149 - BNILDI_09820 0.46 +1.4
1,976,212 - BNILDI_09820 0.55 -1.3
1,976,266 + BNILDI_09820 0.63 -1.0
1,976,270 - BNILDI_09820 0.64 +0.2
1,976,270 - BNILDI_09820 0.64 -2.9
1,976,270 - BNILDI_09820 0.64 +0.3
1,976,309 + BNILDI_09820 0.69 +0.6
1,976,382 + BNILDI_09820 0.79 -0.1
1,976,383 - BNILDI_09820 0.80 -0.1
1,976,383 - BNILDI_09820 0.80 -0.1
1,976,383 - BNILDI_09820 0.80 +0.1
1,976,442 + BNILDI_09820 0.88 -2.6
1,976,779 + BNILDI_09825 0.43 -1.6
1,976,779 + BNILDI_09825 0.43 +0.2
1,976,779 + BNILDI_09825 0.43 +0.7
1,976,782 + BNILDI_09825 0.44 +0.1

Or see this region's nucleotide sequence