Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt araC and yabI are separated by 85 nucleotides yabI and thiQ are separated by 113 nucleotides thiQ and thiP overlap by 17 nucleotides
BNILDI_08490: araC - arabinose operon transcriptional regulator AraC, at 1,699,595 to 1,700,473
araC
BNILDI_08495: yabI - Inner membrane protein YabI, at 1,700,559 to 1,701,323
yabI
BNILDI_08500: thiQ - thiamine ABC transporter ATP-binding protein ThiQ, at 1,701,437 to 1,702,135
thiQ
BNILDI_08505: thiP - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 1,702,119 to 1,703,729
thiP
Position (kb)
1700
1701
1702 Strain fitness (log2 ratio)
-1
0
1 at 1699.686 kb on - strand, within araC at 1699.727 kb on + strand, within araC at 1699.728 kb on - strand, within araC at 1699.728 kb on - strand, within araC at 1699.729 kb on + strand, within araC at 1699.730 kb on - strand, within araC at 1699.730 kb on - strand, within araC at 1699.730 kb on - strand, within araC at 1699.805 kb on - strand, within araC at 1699.918 kb on + strand, within araC at 1699.919 kb on - strand, within araC at 1699.919 kb on - strand, within araC at 1699.932 kb on + strand, within araC at 1700.073 kb on + strand, within araC at 1700.121 kb on - strand, within araC at 1700.122 kb on + strand, within araC at 1700.147 kb on + strand, within araC at 1700.288 kb on - strand, within araC at 1700.372 kb on + strand, within araC at 1700.373 kb on - strand, within araC at 1700.373 kb on - strand, within araC at 1700.386 kb on - strand at 1700.510 kb on + strand at 1700.511 kb on - strand at 1700.516 kb on - strand at 1700.516 kb on - strand at 1700.558 kb on + strand at 1700.559 kb on - strand at 1700.559 kb on - strand at 1700.583 kb on - strand at 1700.595 kb on - strand at 1700.602 kb on - strand at 1701.090 kb on - strand, within yabI at 1701.181 kb on + strand, within yabI at 1701.189 kb on + strand, within yabI at 1701.189 kb on + strand, within yabI at 1701.189 kb on + strand, within yabI at 1701.190 kb on - strand, within yabI at 1701.288 kb on + strand at 1701.328 kb on + strand at 1701.329 kb on - strand at 1701.391 kb on - strand at 1701.435 kb on + strand at 1701.436 kb on - strand at 1701.436 kb on - strand at 1701.436 kb on - strand at 1701.436 kb on - strand at 1701.436 kb on - strand at 1701.588 kb on - strand, within thiQ at 1701.645 kb on - strand, within thiQ at 1701.683 kb on + strand, within thiQ at 1701.776 kb on - strand, within thiQ at 1701.847 kb on + strand, within thiQ at 1702.018 kb on + strand, within thiQ at 1702.019 kb on - strand, within thiQ at 1702.070 kb on + strand at 1702.082 kb on - strand at 1702.182 kb on + strand at 1702.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,699,686 - araC BNILDI_08490 0.10 -1.5 1,699,727 + araC BNILDI_08490 0.15 +0.3 1,699,728 - araC BNILDI_08490 0.15 -0.2 1,699,728 - araC BNILDI_08490 0.15 +0.3 1,699,729 + araC BNILDI_08490 0.15 -0.5 1,699,730 - araC BNILDI_08490 0.15 -0.1 1,699,730 - araC BNILDI_08490 0.15 +0.5 1,699,730 - araC BNILDI_08490 0.15 +0.7 1,699,805 - araC BNILDI_08490 0.24 +0.3 1,699,918 + araC BNILDI_08490 0.37 +0.8 1,699,919 - araC BNILDI_08490 0.37 +0.4 1,699,919 - araC BNILDI_08490 0.37 -0.2 1,699,932 + araC BNILDI_08490 0.38 +0.6 1,700,073 + araC BNILDI_08490 0.54 -0.2 1,700,121 - araC BNILDI_08490 0.60 -0.1 1,700,122 + araC BNILDI_08490 0.60 +0.3 1,700,147 + araC BNILDI_08490 0.63 -0.1 1,700,288 - araC BNILDI_08490 0.79 -0.1 1,700,372 + araC BNILDI_08490 0.88 -0.5 1,700,373 - araC BNILDI_08490 0.89 +0.3 1,700,373 - araC BNILDI_08490 0.89 +0.1 1,700,386 - +0.2 1,700,510 + -0.0 1,700,511 - -0.7 1,700,516 - -0.1 1,700,516 - +0.8 1,700,558 + -0.1 1,700,559 - -0.2 1,700,559 - -1.4 1,700,583 - -0.9 1,700,595 - +0.0 1,700,602 - -0.5 1,701,090 - yabI BNILDI_08495 0.69 -0.4 1,701,181 + yabI BNILDI_08495 0.81 -0.4 1,701,189 + yabI BNILDI_08495 0.82 -0.6 1,701,189 + yabI BNILDI_08495 0.82 -1.5 1,701,189 + yabI BNILDI_08495 0.82 -0.5 1,701,190 - yabI BNILDI_08495 0.82 +1.7 1,701,288 + -0.1 1,701,328 + -0.2 1,701,329 - +0.9 1,701,391 - -1.6 1,701,435 + -0.2 1,701,436 - -1.1 1,701,436 - -1.7 1,701,436 - -0.1 1,701,436 - -0.1 1,701,436 - +0.0 1,701,588 - thiQ BNILDI_08500 0.22 -0.3 1,701,645 - thiQ BNILDI_08500 0.30 -0.7 1,701,683 + thiQ BNILDI_08500 0.35 +0.4 1,701,776 - thiQ BNILDI_08500 0.48 +1.4 1,701,847 + thiQ BNILDI_08500 0.59 -0.2 1,702,018 + thiQ BNILDI_08500 0.83 -0.0 1,702,019 - thiQ BNILDI_08500 0.83 +0.6 1,702,070 + -1.0 1,702,082 - +0.7 1,702,182 + -0.2 1,702,250 - -0.2
Or see this region's nucleotide sequence