Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoB and deoD are separated by 79 nucleotides deoD and BNILDI_08055 are separated by 274 nucleotides BNILDI_08055 and lplA are separated by 31 nucleotides
BNILDI_08045: deoB - phosphopentomutase, at 1,606,480 to 1,607,703
deoB
BNILDI_08050: deoD - purine-nucleoside phosphorylase, at 1,607,783 to 1,608,502
deoD
BNILDI_08055: BNILDI_08055 - Lipoate-protein ligase A, at 1,608,777 to 1,608,926
_08055
BNILDI_08060: lplA - lipoate--protein ligase LplA, at 1,608,958 to 1,609,974
lplA
Position (kb)
1607
1608
1609 Strain fitness (log2 ratio)
-2
-1
0
1 at 1606.826 kb on - strand, within deoB at 1606.984 kb on + strand, within deoB at 1606.984 kb on + strand, within deoB at 1607.095 kb on + strand, within deoB at 1607.096 kb on - strand, within deoB at 1607.269 kb on + strand, within deoB at 1607.269 kb on + strand, within deoB at 1607.269 kb on + strand, within deoB at 1607.637 kb on + strand at 1607.667 kb on + strand at 1607.770 kb on + strand at 1607.771 kb on - strand at 1607.800 kb on + strand at 1607.801 kb on - strand at 1607.801 kb on - strand at 1607.829 kb on + strand at 1607.864 kb on + strand, within deoD at 1607.865 kb on - strand, within deoD at 1607.865 kb on - strand, within deoD at 1607.865 kb on - strand, within deoD at 1607.865 kb on - strand, within deoD at 1607.866 kb on + strand, within deoD at 1607.867 kb on - strand, within deoD at 1607.867 kb on - strand, within deoD at 1607.939 kb on + strand, within deoD at 1607.939 kb on + strand, within deoD at 1607.999 kb on + strand, within deoD at 1608.079 kb on - strand, within deoD at 1608.341 kb on + strand, within deoD at 1608.500 kb on + strand at 1608.501 kb on - strand at 1608.567 kb on + strand at 1608.568 kb on - strand at 1608.571 kb on + strand at 1608.572 kb on - strand at 1608.594 kb on - strand at 1608.696 kb on + strand at 1608.885 kb on + strand, within BNILDI_08055 at 1608.886 kb on - strand, within BNILDI_08055 at 1608.888 kb on - strand, within BNILDI_08055 at 1608.888 kb on - strand, within BNILDI_08055 at 1608.893 kb on + strand, within BNILDI_08055 at 1608.926 kb on + strand at 1609.057 kb on - strand at 1609.327 kb on + strand, within lplA at 1609.440 kb on + strand, within lplA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,606,826 - deoB BNILDI_08045 0.28 -0.8 1,606,984 + deoB BNILDI_08045 0.41 +1.3 1,606,984 + deoB BNILDI_08045 0.41 -2.6 1,607,095 + deoB BNILDI_08045 0.50 +1.2 1,607,096 - deoB BNILDI_08045 0.50 +0.2 1,607,269 + deoB BNILDI_08045 0.64 -0.2 1,607,269 + deoB BNILDI_08045 0.64 -1.5 1,607,269 + deoB BNILDI_08045 0.64 -1.8 1,607,637 + -1.1 1,607,667 + +0.4 1,607,770 + -1.7 1,607,771 - +0.8 1,607,800 + -0.3 1,607,801 - -1.2 1,607,801 - -0.7 1,607,829 + +0.7 1,607,864 + deoD BNILDI_08050 0.11 +0.7 1,607,865 - deoD BNILDI_08050 0.11 -0.3 1,607,865 - deoD BNILDI_08050 0.11 -0.6 1,607,865 - deoD BNILDI_08050 0.11 +0.6 1,607,865 - deoD BNILDI_08050 0.11 -0.9 1,607,866 + deoD BNILDI_08050 0.12 -0.2 1,607,867 - deoD BNILDI_08050 0.12 -0.2 1,607,867 - deoD BNILDI_08050 0.12 -0.8 1,607,939 + deoD BNILDI_08050 0.22 -0.1 1,607,939 + deoD BNILDI_08050 0.22 -0.3 1,607,999 + deoD BNILDI_08050 0.30 +0.0 1,608,079 - deoD BNILDI_08050 0.41 -1.0 1,608,341 + deoD BNILDI_08050 0.78 +0.1 1,608,500 + -0.5 1,608,501 - -1.4 1,608,567 + -0.3 1,608,568 - -0.2 1,608,571 + +0.8 1,608,572 - +0.3 1,608,594 - -0.8 1,608,696 + -0.1 1,608,885 + BNILDI_08055 0.72 -1.7 1,608,886 - BNILDI_08055 0.73 -0.3 1,608,888 - BNILDI_08055 0.74 +0.5 1,608,888 - BNILDI_08055 0.74 +0.0 1,608,893 + BNILDI_08055 0.77 -0.6 1,608,926 + +1.1 1,609,057 - -0.7 1,609,327 + lplA BNILDI_08060 0.36 +0.5 1,609,440 + lplA BNILDI_08060 0.47 -0.0
Or see this region's nucleotide sequence