Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt opgB and yjjA are separated by 253 nucleotides yjjA and dnaC are separated by 48 nucleotides dnaC and dnaT are separated by 2 nucleotides
BNILDI_07915: opgB - phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase, at 1,584,331 to 1,586,622
opgB
BNILDI_07920: yjjA - Uncharacterized protein YjjA, at 1,586,876 to 1,587,370
yjjA
BNILDI_07925: dnaC - DNA replication protein DnaC, at 1,587,419 to 1,588,156
dnaC
BNILDI_07930: dnaT - primosomal protein DnaT, at 1,588,159 to 1,588,698
dnaT
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1585.958 kb on - strand, within opgB at 1586.096 kb on + strand, within opgB at 1586.097 kb on - strand, within opgB at 1586.097 kb on - strand, within opgB at 1586.097 kb on - strand, within opgB at 1586.125 kb on - strand, within opgB at 1586.126 kb on + strand, within opgB at 1586.126 kb on + strand, within opgB at 1586.127 kb on - strand, within opgB at 1586.172 kb on + strand, within opgB at 1586.175 kb on - strand, within opgB at 1586.396 kb on + strand at 1586.397 kb on - strand at 1586.397 kb on - strand at 1586.458 kb on - strand at 1586.500 kb on + strand at 1586.513 kb on - strand at 1586.553 kb on + strand at 1586.554 kb on - strand at 1586.554 kb on - strand at 1586.573 kb on + strand at 1586.574 kb on - strand at 1586.699 kb on + strand at 1586.700 kb on - strand at 1586.753 kb on - strand at 1587.022 kb on - strand, within yjjA at 1587.171 kb on - strand, within yjjA at 1587.200 kb on - strand, within yjjA at 1587.200 kb on - strand, within yjjA at 1587.200 kb on - strand, within yjjA at 1587.204 kb on - strand, within yjjA at 1587.400 kb on - strand at 1587.402 kb on - strand at 1587.421 kb on - strand at 1587.421 kb on - strand at 1587.423 kb on + strand at 1587.423 kb on + strand at 1587.423 kb on + strand at 1587.431 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,585,958 - opgB BNILDI_07915 0.71 +0.3 1,586,096 + opgB BNILDI_07915 0.77 +0.2 1,586,097 - opgB BNILDI_07915 0.77 +0.3 1,586,097 - opgB BNILDI_07915 0.77 +0.1 1,586,097 - opgB BNILDI_07915 0.77 -0.2 1,586,125 - opgB BNILDI_07915 0.78 +0.0 1,586,126 + opgB BNILDI_07915 0.78 +0.2 1,586,126 + opgB BNILDI_07915 0.78 +0.2 1,586,127 - opgB BNILDI_07915 0.78 +0.4 1,586,172 + opgB BNILDI_07915 0.80 -0.2 1,586,175 - opgB BNILDI_07915 0.80 -0.4 1,586,396 + +0.3 1,586,397 - -0.8 1,586,397 - +0.2 1,586,458 - +0.0 1,586,500 + -1.6 1,586,513 - +0.7 1,586,553 + -0.1 1,586,554 - -0.6 1,586,554 - +0.5 1,586,573 + -0.1 1,586,574 - +0.4 1,586,699 + +0.3 1,586,700 - -0.1 1,586,753 - -0.2 1,587,022 - yjjA BNILDI_07920 0.29 -0.2 1,587,171 - yjjA BNILDI_07920 0.60 -1.6 1,587,200 - yjjA BNILDI_07920 0.65 -3.4 1,587,200 - yjjA BNILDI_07920 0.65 +0.2 1,587,200 - yjjA BNILDI_07920 0.65 +0.1 1,587,204 - yjjA BNILDI_07920 0.66 +0.4 1,587,400 - +1.2 1,587,402 - -0.3 1,587,421 - +0.1 1,587,421 - +0.7 1,587,423 + -2.0 1,587,423 + -0.1 1,587,423 + -1.8 1,587,431 - -1.7
Or see this region's nucleotide sequence