Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdD and treC are separated by 393 nucleotides treC and treB are separated by 49 nucleotides
BNILDI_07340: nrdD - anaerobic ribonucleoside-triphosphate reductase, at 1,462,503 to 1,464,641
nrdD
BNILDI_07345: treC - alpha,alpha-phosphotrehalase, at 1,465,035 to 1,466,690
treC
BNILDI_07350: treB - PTS trehalose transporter subunit IIBC, at 1,466,740 to 1,468,161
treB
Position (kb)
1465
1466
1467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1464.223 kb on + strand, within nrdD at 1464.400 kb on + strand, within nrdD at 1464.422 kb on - strand, within nrdD at 1464.530 kb on - strand at 1464.530 kb on - strand at 1464.558 kb on - strand at 1464.558 kb on - strand at 1464.652 kb on + strand at 1464.658 kb on + strand at 1464.785 kb on - strand at 1464.791 kb on + strand at 1464.792 kb on - strand at 1464.792 kb on - strand at 1464.792 kb on - strand at 1464.792 kb on - strand at 1464.811 kb on + strand at 1464.838 kb on + strand at 1464.841 kb on - strand at 1464.843 kb on - strand at 1464.884 kb on - strand at 1465.033 kb on - strand at 1465.036 kb on + strand at 1465.036 kb on + strand at 1465.036 kb on + strand at 1465.037 kb on - strand at 1465.112 kb on + strand at 1465.233 kb on + strand, within treC at 1465.373 kb on - strand, within treC at 1465.377 kb on - strand, within treC at 1465.562 kb on + strand, within treC at 1465.724 kb on + strand, within treC at 1466.482 kb on + strand, within treC at 1466.524 kb on + strand, within treC at 1466.562 kb on - strand at 1466.596 kb on - strand at 1466.691 kb on + strand at 1466.781 kb on - strand at 1466.781 kb on - strand at 1466.785 kb on - strand at 1466.797 kb on + strand at 1466.839 kb on + strand at 1466.840 kb on - strand at 1466.927 kb on - strand, within treB at 1466.927 kb on - strand, within treB at 1466.929 kb on + strand, within treB at 1466.929 kb on + strand, within treB at 1466.930 kb on - strand, within treB at 1466.930 kb on - strand, within treB at 1466.930 kb on - strand, within treB at 1466.996 kb on + strand, within treB at 1466.997 kb on - strand, within treB at 1466.997 kb on - strand, within treB at 1467.092 kb on - strand, within treB at 1467.199 kb on + strand, within treB at 1467.284 kb on - strand, within treB at 1467.415 kb on - strand, within treB at 1467.653 kb on + strand, within treB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,464,223 + nrdD BNILDI_07340 0.80 +0.1 1,464,400 + nrdD BNILDI_07340 0.89 -0.1 1,464,422 - nrdD BNILDI_07340 0.90 +0.1 1,464,530 - +0.4 1,464,530 - -0.4 1,464,558 - -1.0 1,464,558 - -0.1 1,464,652 + +0.3 1,464,658 + -0.0 1,464,785 - +0.0 1,464,791 + -0.1 1,464,792 - +1.1 1,464,792 - +0.9 1,464,792 - -0.3 1,464,792 - -0.7 1,464,811 + -1.1 1,464,838 + -1.0 1,464,841 - -0.9 1,464,843 - +0.7 1,464,884 - +0.1 1,465,033 - +0.2 1,465,036 + -0.6 1,465,036 + +0.1 1,465,036 + -0.1 1,465,037 - -0.4 1,465,112 + -1.6 1,465,233 + treC BNILDI_07345 0.12 -0.8 1,465,373 - treC BNILDI_07345 0.20 +2.0 1,465,377 - treC BNILDI_07345 0.21 +0.6 1,465,562 + treC BNILDI_07345 0.32 -1.6 1,465,724 + treC BNILDI_07345 0.42 -1.8 1,466,482 + treC BNILDI_07345 0.87 -0.8 1,466,524 + treC BNILDI_07345 0.90 -0.7 1,466,562 - +0.4 1,466,596 - -1.8 1,466,691 + -0.6 1,466,781 - +0.1 1,466,781 - +0.1 1,466,785 - +0.0 1,466,797 + -0.7 1,466,839 + +1.0 1,466,840 - -1.7 1,466,927 - treB BNILDI_07350 0.13 -0.5 1,466,927 - treB BNILDI_07350 0.13 -0.2 1,466,929 + treB BNILDI_07350 0.13 -1.0 1,466,929 + treB BNILDI_07350 0.13 -0.8 1,466,930 - treB BNILDI_07350 0.13 +0.1 1,466,930 - treB BNILDI_07350 0.13 +0.1 1,466,930 - treB BNILDI_07350 0.13 -0.2 1,466,996 + treB BNILDI_07350 0.18 -1.7 1,466,997 - treB BNILDI_07350 0.18 +0.1 1,466,997 - treB BNILDI_07350 0.18 +0.6 1,467,092 - treB BNILDI_07350 0.25 +0.1 1,467,199 + treB BNILDI_07350 0.32 +0.1 1,467,284 - treB BNILDI_07350 0.38 +0.4 1,467,415 - treB BNILDI_07350 0.47 +0.3 1,467,653 + treB BNILDI_07350 0.64 +0.3
Or see this region's nucleotide sequence