Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpdB and cysQ are separated by 189 nucleotides cysQ and ytfJ overlap by 11 nucleotides ytfJ and ytfK are separated by 324 nucleotides
BNILDI_07215: cpdB - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 1,437,717 to 1,439,660
cpdB
BNILDI_07225: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 1,439,850 to 1,440,590
cysQ
BNILDI_07230: ytfJ - Uncharacterized protein YtfJ, at 1,440,580 to 1,441,137
ytfJ
BNILDI_07235: ytfK - Uncharacterized protein YtfK, at 1,441,462 to 1,441,668
ytfK
Position (kb)
1439
1440
1441 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1438.902 kb on - strand, within cpdB at 1438.902 kb on - strand, within cpdB at 1438.902 kb on - strand, within cpdB at 1438.933 kb on + strand, within cpdB at 1438.934 kb on - strand, within cpdB at 1438.934 kb on - strand, within cpdB at 1438.937 kb on - strand, within cpdB at 1438.963 kb on + strand, within cpdB at 1438.963 kb on + strand, within cpdB at 1438.964 kb on - strand, within cpdB at 1439.038 kb on + strand, within cpdB at 1439.038 kb on + strand, within cpdB at 1439.038 kb on + strand, within cpdB at 1439.038 kb on + strand, within cpdB at 1439.090 kb on + strand, within cpdB at 1439.133 kb on - strand, within cpdB at 1439.189 kb on + strand, within cpdB at 1439.189 kb on + strand, within cpdB at 1439.189 kb on + strand, within cpdB at 1439.193 kb on + strand, within cpdB at 1439.193 kb on + strand, within cpdB at 1439.198 kb on - strand, within cpdB at 1439.205 kb on - strand, within cpdB at 1439.205 kb on - strand, within cpdB at 1439.247 kb on + strand, within cpdB at 1439.247 kb on + strand, within cpdB at 1439.248 kb on - strand, within cpdB at 1439.303 kb on + strand, within cpdB at 1439.336 kb on - strand, within cpdB at 1439.350 kb on - strand, within cpdB at 1439.360 kb on - strand, within cpdB at 1439.360 kb on - strand, within cpdB at 1439.360 kb on - strand, within cpdB at 1439.372 kb on + strand, within cpdB at 1439.385 kb on + strand, within cpdB at 1439.404 kb on + strand, within cpdB at 1439.404 kb on + strand, within cpdB at 1439.404 kb on + strand, within cpdB at 1439.498 kb on + strand at 1439.502 kb on + strand at 1439.502 kb on + strand at 1439.503 kb on - strand at 1439.503 kb on - strand at 1439.562 kb on - strand at 1439.585 kb on + strand at 1439.655 kb on - strand at 1439.655 kb on - strand at 1439.706 kb on + strand at 1439.715 kb on + strand at 1439.716 kb on - strand at 1439.716 kb on - strand at 1439.716 kb on - strand at 1439.719 kb on + strand at 1439.719 kb on + strand at 1439.720 kb on - strand at 1439.720 kb on - strand at 1439.721 kb on + strand at 1439.722 kb on - strand at 1439.864 kb on - strand at 1439.897 kb on + strand at 1439.898 kb on - strand at 1439.898 kb on - strand at 1440.004 kb on + strand, within cysQ at 1440.125 kb on + strand, within cysQ at 1440.126 kb on - strand, within cysQ at 1440.126 kb on - strand, within cysQ at 1440.195 kb on + strand, within cysQ at 1440.196 kb on - strand, within cysQ at 1440.196 kb on - strand, within cysQ at 1440.214 kb on + strand, within cysQ at 1440.214 kb on + strand, within cysQ at 1440.214 kb on + strand, within cysQ at 1440.215 kb on - strand, within cysQ at 1440.220 kb on - strand, within cysQ at 1440.538 kb on - strand at 1440.581 kb on + strand at 1440.581 kb on + strand at 1440.581 kb on + strand at 1440.582 kb on - strand at 1440.585 kb on + strand at 1440.585 kb on + strand at 1440.586 kb on - strand at 1440.592 kb on + strand at 1440.593 kb on - strand at 1440.593 kb on - strand at 1440.597 kb on + strand at 1440.689 kb on - strand, within ytfJ at 1440.939 kb on + strand, within ytfJ at 1440.939 kb on + strand, within ytfJ at 1440.939 kb on + strand, within ytfJ at 1440.939 kb on + strand, within ytfJ at 1440.949 kb on + strand, within ytfJ at 1440.993 kb on - strand, within ytfJ at 1440.997 kb on - strand, within ytfJ at 1441.152 kb on - strand at 1441.190 kb on + strand at 1441.196 kb on + strand at 1441.196 kb on + strand at 1441.196 kb on + strand at 1441.196 kb on + strand at 1441.197 kb on - strand at 1441.197 kb on - strand at 1441.374 kb on + strand at 1441.374 kb on + strand at 1441.374 kb on + strand at 1441.375 kb on - strand at 1441.375 kb on - strand at 1441.375 kb on - strand at 1441.375 kb on - strand at 1441.376 kb on + strand at 1441.406 kb on + strand at 1441.407 kb on - strand at 1441.409 kb on + strand at 1441.440 kb on + strand at 1441.440 kb on + strand at 1441.440 kb on + strand at 1441.534 kb on + strand, within ytfK at 1441.534 kb on + strand, within ytfK at 1441.534 kb on + strand, within ytfK at 1441.535 kb on - strand, within ytfK at 1441.535 kb on - strand, within ytfK at 1441.535 kb on - strand, within ytfK at 1441.535 kb on - strand, within ytfK at 1441.535 kb on - strand, within ytfK at 1441.535 kb on - strand, within ytfK
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,438,902 - cpdB BNILDI_07215 0.61 +0.6 1,438,902 - cpdB BNILDI_07215 0.61 +0.1 1,438,902 - cpdB BNILDI_07215 0.61 -0.0 1,438,933 + cpdB BNILDI_07215 0.63 -1.2 1,438,934 - cpdB BNILDI_07215 0.63 +0.3 1,438,934 - cpdB BNILDI_07215 0.63 +0.5 1,438,937 - cpdB BNILDI_07215 0.63 +0.2 1,438,963 + cpdB BNILDI_07215 0.64 -1.4 1,438,963 + cpdB BNILDI_07215 0.64 +1.8 1,438,964 - cpdB BNILDI_07215 0.64 +0.2 1,439,038 + cpdB BNILDI_07215 0.68 -1.1 1,439,038 + cpdB BNILDI_07215 0.68 -0.3 1,439,038 + cpdB BNILDI_07215 0.68 +0.3 1,439,038 + cpdB BNILDI_07215 0.68 -0.1 1,439,090 + cpdB BNILDI_07215 0.71 +0.2 1,439,133 - cpdB BNILDI_07215 0.73 +0.2 1,439,189 + cpdB BNILDI_07215 0.76 -0.2 1,439,189 + cpdB BNILDI_07215 0.76 -0.8 1,439,189 + cpdB BNILDI_07215 0.76 -0.6 1,439,193 + cpdB BNILDI_07215 0.76 +0.4 1,439,193 + cpdB BNILDI_07215 0.76 +0.2 1,439,198 - cpdB BNILDI_07215 0.76 +0.3 1,439,205 - cpdB BNILDI_07215 0.77 +0.1 1,439,205 - cpdB BNILDI_07215 0.77 -0.7 1,439,247 + cpdB BNILDI_07215 0.79 +0.3 1,439,247 + cpdB BNILDI_07215 0.79 -0.3 1,439,248 - cpdB BNILDI_07215 0.79 -0.7 1,439,303 + cpdB BNILDI_07215 0.82 -0.0 1,439,336 - cpdB BNILDI_07215 0.83 -0.9 1,439,350 - cpdB BNILDI_07215 0.84 -1.0 1,439,360 - cpdB BNILDI_07215 0.85 -0.2 1,439,360 - cpdB BNILDI_07215 0.85 +0.6 1,439,360 - cpdB BNILDI_07215 0.85 -0.1 1,439,372 + cpdB BNILDI_07215 0.85 +0.2 1,439,385 + cpdB BNILDI_07215 0.86 +0.3 1,439,404 + cpdB BNILDI_07215 0.87 +0.2 1,439,404 + cpdB BNILDI_07215 0.87 +0.1 1,439,404 + cpdB BNILDI_07215 0.87 -0.8 1,439,498 + +0.2 1,439,502 + +0.7 1,439,502 + -0.6 1,439,503 - -0.0 1,439,503 - +0.9 1,439,562 - -0.2 1,439,585 + +0.1 1,439,655 - -0.3 1,439,655 - -0.2 1,439,706 + -1.2 1,439,715 + +0.4 1,439,716 - -2.1 1,439,716 - -1.1 1,439,716 - -2.4 1,439,719 + +0.9 1,439,719 + -0.0 1,439,720 - +0.1 1,439,720 - +1.1 1,439,721 + -0.5 1,439,722 - +0.3 1,439,864 - -0.2 1,439,897 + -0.3 1,439,898 - +0.5 1,439,898 - +0.2 1,440,004 + cysQ BNILDI_07225 0.21 -0.5 1,440,125 + cysQ BNILDI_07225 0.37 +0.1 1,440,126 - cysQ BNILDI_07225 0.37 -0.2 1,440,126 - cysQ BNILDI_07225 0.37 +0.8 1,440,195 + cysQ BNILDI_07225 0.47 -0.3 1,440,196 - cysQ BNILDI_07225 0.47 -2.4 1,440,196 - cysQ BNILDI_07225 0.47 +0.9 1,440,214 + cysQ BNILDI_07225 0.49 +0.5 1,440,214 + cysQ BNILDI_07225 0.49 +0.1 1,440,214 + cysQ BNILDI_07225 0.49 +1.3 1,440,215 - cysQ BNILDI_07225 0.49 +0.7 1,440,220 - cysQ BNILDI_07225 0.50 +0.7 1,440,538 - -0.1 1,440,581 + +0.5 1,440,581 + +0.2 1,440,581 + +0.5 1,440,582 - +0.4 1,440,585 + -0.1 1,440,585 + +0.3 1,440,586 - -0.3 1,440,592 + +0.3 1,440,593 - -0.1 1,440,593 - -0.8 1,440,597 + -1.4 1,440,689 - ytfJ BNILDI_07230 0.20 -0.5 1,440,939 + ytfJ BNILDI_07230 0.64 +0.4 1,440,939 + ytfJ BNILDI_07230 0.64 +0.1 1,440,939 + ytfJ BNILDI_07230 0.64 -0.1 1,440,939 + ytfJ BNILDI_07230 0.64 +0.8 1,440,949 + ytfJ BNILDI_07230 0.66 -0.9 1,440,993 - ytfJ BNILDI_07230 0.74 +0.2 1,440,997 - ytfJ BNILDI_07230 0.75 -0.3 1,441,152 - -0.5 1,441,190 + -0.8 1,441,196 + -0.2 1,441,196 + +1.2 1,441,196 + -0.2 1,441,196 + +1.4 1,441,197 - -0.8 1,441,197 - -2.9 1,441,374 + +1.0 1,441,374 + +0.0 1,441,374 + -1.0 1,441,375 - +0.8 1,441,375 - +0.6 1,441,375 - +0.6 1,441,375 - -0.2 1,441,376 + -0.8 1,441,406 + +0.1 1,441,407 - +0.1 1,441,409 + -1.5 1,441,440 + +0.1 1,441,440 + +1.2 1,441,440 + +0.5 1,441,534 + ytfK BNILDI_07235 0.35 -1.0 1,441,534 + ytfK BNILDI_07235 0.35 -1.3 1,441,534 + ytfK BNILDI_07235 0.35 +0.6 1,441,535 - ytfK BNILDI_07235 0.35 -0.5 1,441,535 - ytfK BNILDI_07235 0.35 +0.4 1,441,535 - ytfK BNILDI_07235 0.35 +2.0 1,441,535 - ytfK BNILDI_07235 0.35 -1.1 1,441,535 - ytfK BNILDI_07235 0.35 -1.8 1,441,535 - ytfK BNILDI_07235 0.35 +0.0
Or see this region's nucleotide sequence