Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaE and ulaF overlap by 1 nucleotides ulaF and BNILDI_07105 are separated by 128 nucleotides BNILDI_07105 and rpsF are separated by 326 nucleotides rpsF and priB are separated by 6 nucleotides
BNILDI_07095: ulaE - L-ribulose-5-phosphate 3-epimerase UlaE, at 1,417,589 to 1,418,443
ulaE
BNILDI_07100: ulaF - L-ribulose-5-phosphate 4-epimerase UlaF, at 1,418,443 to 1,419,129
ulaF
BNILDI_07105: BNILDI_07105 - Uncharacterized protein YjfY, at 1,419,258 to 1,419,533
_07105
BNILDI_07110: rpsF - 30S ribosomal protein S6, at 1,419,860 to 1,420,255
rpsF
BNILDI_07115: priB - primosomal replication protein N, at 1,420,262 to 1,420,576
priB
Position (kb)
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1418.275 kb on - strand, within ulaE at 1418.318 kb on + strand, within ulaE at 1418.330 kb on + strand, within ulaE at 1418.331 kb on - strand, within ulaE at 1418.466 kb on - strand at 1418.686 kb on + strand, within ulaF at 1418.721 kb on + strand, within ulaF at 1418.778 kb on - strand, within ulaF at 1418.936 kb on - strand, within ulaF at 1418.936 kb on - strand, within ulaF at 1419.037 kb on - strand, within ulaF at 1419.093 kb on + strand at 1419.164 kb on + strand at 1419.164 kb on + strand at 1419.165 kb on - strand at 1419.174 kb on - strand at 1419.227 kb on + strand at 1419.228 kb on - strand at 1419.265 kb on + strand at 1419.287 kb on + strand, within BNILDI_07105 at 1419.322 kb on - strand, within BNILDI_07105 at 1419.328 kb on + strand, within BNILDI_07105 at 1419.329 kb on - strand, within BNILDI_07105 at 1419.329 kb on - strand, within BNILDI_07105 at 1419.331 kb on + strand, within BNILDI_07105 at 1419.331 kb on + strand, within BNILDI_07105 at 1419.331 kb on + strand, within BNILDI_07105 at 1419.332 kb on - strand, within BNILDI_07105 at 1419.332 kb on - strand, within BNILDI_07105 at 1419.364 kb on - strand, within BNILDI_07105 at 1419.409 kb on + strand, within BNILDI_07105 at 1419.482 kb on + strand, within BNILDI_07105 at 1419.483 kb on - strand, within BNILDI_07105 at 1419.533 kb on + strand at 1419.533 kb on + strand at 1419.534 kb on - strand at 1419.534 kb on - strand at 1419.534 kb on - strand at 1419.537 kb on + strand at 1419.537 kb on + strand at 1419.697 kb on + strand at 1419.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,418,275 - ulaE BNILDI_07095 0.80 +0.8 1,418,318 + ulaE BNILDI_07095 0.85 +0.1 1,418,330 + ulaE BNILDI_07095 0.87 -0.1 1,418,331 - ulaE BNILDI_07095 0.87 -1.4 1,418,466 - -0.5 1,418,686 + ulaF BNILDI_07100 0.35 -0.2 1,418,721 + ulaF BNILDI_07100 0.40 +0.4 1,418,778 - ulaF BNILDI_07100 0.49 +0.2 1,418,936 - ulaF BNILDI_07100 0.72 +1.5 1,418,936 - ulaF BNILDI_07100 0.72 +0.6 1,419,037 - ulaF BNILDI_07100 0.86 +0.5 1,419,093 + -0.3 1,419,164 + +1.0 1,419,164 + +0.1 1,419,165 - +0.0 1,419,174 - +1.0 1,419,227 + +2.4 1,419,228 - -0.3 1,419,265 + +1.3 1,419,287 + BNILDI_07105 0.11 -0.1 1,419,322 - BNILDI_07105 0.23 +0.7 1,419,328 + BNILDI_07105 0.25 +0.3 1,419,329 - BNILDI_07105 0.26 +0.1 1,419,329 - BNILDI_07105 0.26 -0.2 1,419,331 + BNILDI_07105 0.26 -1.2 1,419,331 + BNILDI_07105 0.26 -0.5 1,419,331 + BNILDI_07105 0.26 -0.6 1,419,332 - BNILDI_07105 0.27 -0.4 1,419,332 - BNILDI_07105 0.27 -0.4 1,419,364 - BNILDI_07105 0.38 -0.6 1,419,409 + BNILDI_07105 0.55 -1.1 1,419,482 + BNILDI_07105 0.81 +0.3 1,419,483 - BNILDI_07105 0.82 +0.3 1,419,533 + +0.6 1,419,533 + -2.8 1,419,534 - +0.1 1,419,534 - +2.0 1,419,534 - -0.3 1,419,537 + +0.1 1,419,537 + -0.6 1,419,697 + -0.1 1,419,718 - +1.4
Or see this region's nucleotide sequence