Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt blaEC and frdD are separated by 62 nucleotides frdD and BNILDI_06875 are separated by 10 nucleotides BNILDI_06875 and frdB are separated by 10 nucleotides frdB and frdA overlap by 8 nucleotides
BNILDI_06865: blaEC - BlaEC family class C beta-lactamase, at 1,373,108 to 1,374,241
blaEC
BNILDI_06870: frdD - fumarate reductase subunit FrdD, at 1,374,304 to 1,374,663
frdD
BNILDI_06875: BNILDI_06875 - Fumarate reductase subunit C, at 1,374,674 to 1,375,069
_06875
BNILDI_06880: frdB - fumarate reductase iron-sulfur protein, at 1,375,080 to 1,375,814
frdB
BNILDI_06885: frdA - fumarate reductase (quinol) flavoprotein subunit, at 1,375,807 to 1,377,615
frdA
Position (kb)
1374
1375
1376 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1373.745 kb on + strand, within blaEC at 1373.746 kb on - strand, within blaEC at 1373.873 kb on + strand, within blaEC at 1373.873 kb on + strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.874 kb on - strand, within blaEC at 1373.876 kb on + strand, within blaEC at 1373.877 kb on - strand, within blaEC at 1373.940 kb on - strand, within blaEC at 1374.075 kb on + strand, within blaEC at 1374.075 kb on + strand, within blaEC at 1374.076 kb on - strand, within blaEC at 1374.076 kb on - strand, within blaEC at 1374.079 kb on - strand, within blaEC at 1374.134 kb on + strand at 1374.135 kb on - strand at 1374.145 kb on + strand at 1374.146 kb on - strand at 1374.164 kb on - strand at 1374.209 kb on - strand at 1374.212 kb on + strand at 1374.212 kb on + strand at 1374.212 kb on + strand at 1374.241 kb on + strand at 1374.241 kb on + strand at 1374.241 kb on + strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.242 kb on - strand at 1374.257 kb on + strand at 1374.300 kb on + strand at 1374.313 kb on - strand at 1374.417 kb on + strand, within frdD at 1374.424 kb on + strand, within frdD at 1374.424 kb on + strand, within frdD at 1374.425 kb on - strand, within frdD at 1374.598 kb on - strand, within frdD at 1374.598 kb on - strand, within frdD at 1374.625 kb on + strand, within frdD at 1374.657 kb on + strand at 1374.658 kb on - strand at 1374.682 kb on - strand at 1374.701 kb on + strand at 1374.774 kb on - strand, within BNILDI_06875 at 1374.994 kb on - strand, within BNILDI_06875 at 1375.047 kb on + strand at 1375.047 kb on + strand at 1375.048 kb on - strand at 1375.048 kb on - strand at 1375.061 kb on - strand at 1375.126 kb on - strand at 1375.209 kb on + strand, within frdB at 1375.209 kb on + strand, within frdB at 1375.238 kb on + strand, within frdB at 1375.276 kb on + strand, within frdB at 1375.387 kb on + strand, within frdB at 1375.422 kb on - strand, within frdB at 1375.529 kb on + strand, within frdB at 1375.529 kb on + strand, within frdB at 1375.530 kb on - strand, within frdB at 1375.550 kb on + strand, within frdB at 1375.551 kb on - strand, within frdB at 1375.561 kb on + strand, within frdB at 1375.562 kb on - strand, within frdB at 1375.580 kb on + strand, within frdB at 1375.581 kb on - strand, within frdB at 1375.581 kb on - strand, within frdB at 1375.638 kb on - strand, within frdB at 1375.688 kb on - strand, within frdB at 1375.712 kb on + strand, within frdB at 1375.712 kb on + strand, within frdB at 1375.713 kb on - strand, within frdB at 1375.723 kb on + strand, within frdB at 1375.735 kb on + strand, within frdB at 1375.735 kb on + strand, within frdB at 1375.736 kb on - strand, within frdB at 1375.773 kb on - strand at 1375.774 kb on + strand at 1375.830 kb on + strand at 1375.830 kb on + strand at 1375.885 kb on - strand at 1375.899 kb on + strand at 1375.902 kb on + strand at 1375.902 kb on + strand at 1375.919 kb on + strand at 1376.038 kb on - strand, within frdA at 1376.038 kb on - strand, within frdA at 1376.038 kb on - strand, within frdA at 1376.038 kb on - strand, within frdA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,373,745 + blaEC BNILDI_06865 0.56 -2.3 1,373,746 - blaEC BNILDI_06865 0.56 +1.7 1,373,873 + blaEC BNILDI_06865 0.67 -0.7 1,373,873 + blaEC BNILDI_06865 0.67 -2.9 1,373,874 - blaEC BNILDI_06865 0.68 -0.5 1,373,874 - blaEC BNILDI_06865 0.68 +0.3 1,373,874 - blaEC BNILDI_06865 0.68 -0.8 1,373,874 - blaEC BNILDI_06865 0.68 +0.4 1,373,876 + blaEC BNILDI_06865 0.68 -2.7 1,373,877 - blaEC BNILDI_06865 0.68 -0.8 1,373,940 - blaEC BNILDI_06865 0.73 +0.5 1,374,075 + blaEC BNILDI_06865 0.85 -0.2 1,374,075 + blaEC BNILDI_06865 0.85 +0.6 1,374,076 - blaEC BNILDI_06865 0.85 -0.8 1,374,076 - blaEC BNILDI_06865 0.85 -0.1 1,374,079 - blaEC BNILDI_06865 0.86 -0.1 1,374,134 + +0.1 1,374,135 - -0.8 1,374,145 + -0.7 1,374,146 - -1.1 1,374,164 - +0.4 1,374,209 - +0.3 1,374,212 + +0.1 1,374,212 + +0.3 1,374,212 + +0.1 1,374,241 + -0.3 1,374,241 + -0.2 1,374,241 + -1.5 1,374,242 - +0.1 1,374,242 - -2.3 1,374,242 - -0.3 1,374,242 - -2.0 1,374,257 + +0.4 1,374,300 + -0.5 1,374,313 - -1.5 1,374,417 + frdD BNILDI_06870 0.31 +0.5 1,374,424 + frdD BNILDI_06870 0.33 -1.6 1,374,424 + frdD BNILDI_06870 0.33 +0.1 1,374,425 - frdD BNILDI_06870 0.34 +0.6 1,374,598 - frdD BNILDI_06870 0.82 -1.4 1,374,598 - frdD BNILDI_06870 0.82 -1.6 1,374,625 + frdD BNILDI_06870 0.89 +0.7 1,374,657 + +0.8 1,374,658 - +0.4 1,374,682 - +0.4 1,374,701 + +0.2 1,374,774 - BNILDI_06875 0.25 +1.2 1,374,994 - BNILDI_06875 0.81 +0.8 1,375,047 + +0.2 1,375,047 + +1.1 1,375,048 - +0.3 1,375,048 - -0.4 1,375,061 - +0.2 1,375,126 - -0.5 1,375,209 + frdB BNILDI_06880 0.18 -0.5 1,375,209 + frdB BNILDI_06880 0.18 +0.9 1,375,238 + frdB BNILDI_06880 0.21 -1.4 1,375,276 + frdB BNILDI_06880 0.27 -0.9 1,375,387 + frdB BNILDI_06880 0.42 +2.0 1,375,422 - frdB BNILDI_06880 0.47 -0.3 1,375,529 + frdB BNILDI_06880 0.61 +0.1 1,375,529 + frdB BNILDI_06880 0.61 +0.2 1,375,530 - frdB BNILDI_06880 0.61 -0.6 1,375,550 + frdB BNILDI_06880 0.64 -0.5 1,375,551 - frdB BNILDI_06880 0.64 +0.0 1,375,561 + frdB BNILDI_06880 0.65 +0.4 1,375,562 - frdB BNILDI_06880 0.66 +0.0 1,375,580 + frdB BNILDI_06880 0.68 +0.3 1,375,581 - frdB BNILDI_06880 0.68 +0.7 1,375,581 - frdB BNILDI_06880 0.68 -0.4 1,375,638 - frdB BNILDI_06880 0.76 +0.0 1,375,688 - frdB BNILDI_06880 0.83 +0.1 1,375,712 + frdB BNILDI_06880 0.86 -1.0 1,375,712 + frdB BNILDI_06880 0.86 -0.4 1,375,713 - frdB BNILDI_06880 0.86 +0.9 1,375,723 + frdB BNILDI_06880 0.87 -0.5 1,375,735 + frdB BNILDI_06880 0.89 -1.8 1,375,735 + frdB BNILDI_06880 0.89 -0.6 1,375,736 - frdB BNILDI_06880 0.89 +0.5 1,375,773 - -0.5 1,375,774 + +1.2 1,375,830 + +0.4 1,375,830 + +0.2 1,375,885 - +0.2 1,375,899 + -0.8 1,375,902 + +1.2 1,375,902 + -0.2 1,375,919 + -1.1 1,376,038 - frdA BNILDI_06885 0.13 +0.2 1,376,038 - frdA BNILDI_06885 0.13 -0.8 1,376,038 - frdA BNILDI_06885 0.13 +1.0 1,376,038 - frdA BNILDI_06885 0.13 +1.7
Or see this region's nucleotide sequence