Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cdh and tpiA are separated by 54 nucleotides tpiA and yiiQ are separated by 107 nucleotides yiiQ and yiiR are separated by 100 nucleotides yiiR and yfcZ are separated by 211 nucleotides
BNILDI_05580: cdh - CDP-diacylglycerol diphosphatase, at 1,102,778 to 1,103,533
cdh
BNILDI_05585: tpiA - triose-phosphate isomerase, at 1,103,588 to 1,104,355
tpiA
BNILDI_05590: yiiQ - Uncharacterized protein YiiQ, at 1,104,463 to 1,105,062
yiiQ
BNILDI_05595: yiiR - Uncharacterized protein YiiR, at 1,105,163 to 1,105,603
yiiR
BNILDI_05600: yfcZ - DUF406 domain-containing protein, at 1,105,815 to 1,106,114
yfcZ
Position (kb)
1104
1105
1106 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1103.469 kb on + strand at 1103.553 kb on + strand at 1103.554 kb on - strand at 1103.554 kb on - strand at 1103.554 kb on - strand at 1103.554 kb on - strand at 1103.590 kb on - strand at 1103.590 kb on - strand at 1103.590 kb on - strand at 1103.590 kb on - strand at 1103.707 kb on + strand, within tpiA at 1103.718 kb on - strand, within tpiA at 1104.372 kb on + strand at 1104.375 kb on - strand at 1104.430 kb on - strand at 1104.584 kb on + strand, within yiiQ at 1104.584 kb on + strand, within yiiQ at 1104.585 kb on - strand, within yiiQ at 1104.686 kb on + strand, within yiiQ at 1104.686 kb on + strand, within yiiQ at 1104.686 kb on + strand, within yiiQ at 1104.797 kb on + strand, within yiiQ at 1104.797 kb on + strand, within yiiQ at 1104.798 kb on - strand, within yiiQ at 1104.816 kb on + strand, within yiiQ at 1105.030 kb on + strand at 1105.030 kb on + strand at 1105.030 kb on + strand at 1105.030 kb on + strand at 1105.031 kb on - strand at 1105.031 kb on - strand at 1105.048 kb on - strand at 1105.069 kb on + strand at 1105.070 kb on - strand at 1105.128 kb on - strand at 1105.161 kb on - strand at 1105.170 kb on + strand at 1105.170 kb on + strand at 1105.170 kb on + strand at 1105.171 kb on - strand at 1105.552 kb on + strand, within yiiR at 1105.575 kb on - strand at 1105.598 kb on - strand at 1105.611 kb on + strand at 1105.646 kb on - strand at 1105.738 kb on - strand at 1105.774 kb on - strand at 1105.801 kb on + strand at 1105.802 kb on - strand at 1105.802 kb on - strand at 1105.832 kb on + strand at 1105.832 kb on + strand at 1105.842 kb on - strand at 1105.892 kb on + strand, within yfcZ at 1105.892 kb on + strand, within yfcZ at 1105.893 kb on - strand, within yfcZ at 1105.893 kb on - strand, within yfcZ at 1105.898 kb on + strand, within yfcZ at 1105.899 kb on - strand, within yfcZ at 1105.943 kb on + strand, within yfcZ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,103,469 + +0.0 1,103,553 + +0.7 1,103,554 - -0.1 1,103,554 - +0.4 1,103,554 - -0.1 1,103,554 - -3.1 1,103,590 - +0.2 1,103,590 - +0.2 1,103,590 - -0.1 1,103,590 - -0.2 1,103,707 + tpiA BNILDI_05585 0.15 -2.6 1,103,718 - tpiA BNILDI_05585 0.17 -2.4 1,104,372 + -2.6 1,104,375 - +0.1 1,104,430 - +0.3 1,104,584 + yiiQ BNILDI_05590 0.20 -0.2 1,104,584 + yiiQ BNILDI_05590 0.20 +0.4 1,104,585 - yiiQ BNILDI_05590 0.20 -1.1 1,104,686 + yiiQ BNILDI_05590 0.37 -0.1 1,104,686 + yiiQ BNILDI_05590 0.37 +0.4 1,104,686 + yiiQ BNILDI_05590 0.37 -0.1 1,104,797 + yiiQ BNILDI_05590 0.56 -0.3 1,104,797 + yiiQ BNILDI_05590 0.56 -0.2 1,104,798 - yiiQ BNILDI_05590 0.56 +1.4 1,104,816 + yiiQ BNILDI_05590 0.59 +0.9 1,105,030 + +0.2 1,105,030 + +0.2 1,105,030 + -0.4 1,105,030 + -0.3 1,105,031 - -0.0 1,105,031 - -0.3 1,105,048 - -0.2 1,105,069 + +0.1 1,105,070 - -0.8 1,105,128 - +1.4 1,105,161 - -1.1 1,105,170 + +0.2 1,105,170 + +0.2 1,105,170 + +1.0 1,105,171 - +0.4 1,105,552 + yiiR BNILDI_05595 0.88 +0.2 1,105,575 - +1.1 1,105,598 - -2.2 1,105,611 + +0.3 1,105,646 - +0.7 1,105,738 - +0.2 1,105,774 - -0.2 1,105,801 + -0.2 1,105,802 - -0.1 1,105,802 - -0.3 1,105,832 + -0.4 1,105,832 + +0.9 1,105,842 - -1.5 1,105,892 + yfcZ BNILDI_05600 0.26 +0.7 1,105,892 + yfcZ BNILDI_05600 0.26 +0.4 1,105,893 - yfcZ BNILDI_05600 0.26 -0.5 1,105,893 - yfcZ BNILDI_05600 0.26 -0.2 1,105,898 + yfcZ BNILDI_05600 0.28 -0.5 1,105,899 - yfcZ BNILDI_05600 0.28 -0.1 1,105,943 + yfcZ BNILDI_05600 0.43 -1.5
Or see this region's nucleotide sequence