Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mioC and asnC are separated by 89 nucleotides asnC and asnA are separated by 151 nucleotides asnA and viaA are separated by 4 nucleotides
BNILDI_04610: mioC - FMN-binding protein MioC, at 912,990 to 913,433
mioC
BNILDI_04615: asnC - transcriptional regulator AsnC, at 913,523 to 913,981
asnC
BNILDI_04620: asnA - aspartate--ammonia ligase, at 914,133 to 915,125
asnA
BNILDI_04625: viaA - ATPase RavA stimulator ViaA, at 915,130 to 916,581
viaA
Position (kb) 914
915
916 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 913.150 kb on + strand, within mioC at 913.150 kb on + strand, within mioC at 913.225 kb on + strand, within mioC at 913.226 kb on - strand, within mioC at 913.226 kb on - strand, within mioC at 913.226 kb on - strand, within mioC at 913.250 kb on + strand, within mioC at 913.302 kb on + strand, within mioC at 913.302 kb on + strand, within mioC at 913.340 kb on + strand, within mioC at 913.341 kb on - strand, within mioC at 913.385 kb on - strand, within mioC at 913.415 kb on + strand at 913.415 kb on + strand at 913.416 kb on - strand at 913.416 kb on - strand at 913.425 kb on - strand at 913.447 kb on + strand at 913.451 kb on - strand at 913.466 kb on + strand at 913.467 kb on - strand at 913.504 kb on - strand at 913.613 kb on - strand, within asnC at 913.613 kb on - strand, within asnC at 913.616 kb on + strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.617 kb on - strand, within asnC at 913.655 kb on + strand, within asnC at 913.669 kb on - strand, within asnC at 913.683 kb on + strand, within asnC at 913.683 kb on + strand, within asnC at 913.684 kb on - strand, within asnC at 913.737 kb on + strand, within asnC at 913.757 kb on + strand, within asnC at 913.757 kb on + strand, within asnC at 913.758 kb on - strand, within asnC at 913.759 kb on + strand, within asnC at 913.760 kb on - strand, within asnC at 913.778 kb on + strand, within asnC at 913.779 kb on - strand, within asnC at 913.779 kb on - strand, within asnC at 913.779 kb on - strand, within asnC at 913.813 kb on + strand, within asnC at 913.972 kb on - strand at 913.982 kb on - strand at 914.145 kb on + strand at 914.145 kb on + strand at 914.145 kb on + strand at 914.168 kb on + strand at 914.270 kb on + strand, within asnA at 914.270 kb on + strand, within asnA at 914.271 kb on - strand, within asnA at 914.303 kb on - strand, within asnA at 914.409 kb on + strand, within asnA at 914.409 kb on + strand, within asnA at 914.410 kb on - strand, within asnA at 914.469 kb on + strand, within asnA at 914.469 kb on + strand, within asnA at 914.543 kb on - strand, within asnA at 914.636 kb on - strand, within asnA at 914.650 kb on + strand, within asnA at 914.651 kb on - strand, within asnA at 914.784 kb on + strand, within asnA at 914.785 kb on - strand, within asnA at 914.837 kb on + strand, within asnA at 915.082 kb on + strand at 915.083 kb on - strand at 915.083 kb on - strand at 915.083 kb on - strand at 915.126 kb on + strand at 915.186 kb on + strand at 915.187 kb on - strand at 915.187 kb on - strand at 915.260 kb on - strand at 915.427 kb on + strand, within viaA at 915.497 kb on - strand, within viaA at 915.501 kb on + strand, within viaA at 915.501 kb on + strand, within viaA at 915.502 kb on - strand, within viaA at 915.502 kb on - strand, within viaA at 915.502 kb on - strand, within viaA at 915.504 kb on + strand, within viaA at 915.504 kb on + strand, within viaA at 915.505 kb on - strand, within viaA at 915.505 kb on - strand, within viaA at 915.505 kb on - strand, within viaA at 915.506 kb on + strand, within viaA at 915.506 kb on + strand, within viaA at 915.507 kb on - strand, within viaA at 915.577 kb on - strand, within viaA at 915.648 kb on + strand, within viaA at 915.649 kb on - strand, within viaA at 915.649 kb on - strand, within viaA at 915.649 kb on - strand, within viaA at 915.652 kb on + strand, within viaA at 915.653 kb on - strand, within viaA at 915.705 kb on - strand, within viaA at 915.746 kb on + strand, within viaA at 915.747 kb on - strand, within viaA at 916.027 kb on - strand, within viaA at 916.057 kb on - strand, within viaA at 916.057 kb on - strand, within viaA at 916.115 kb on - strand, within viaA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 913,150 + mioC BNILDI_04610 0.36 +1.2 913,150 + mioC BNILDI_04610 0.36 -0.3 913,225 + mioC BNILDI_04610 0.53 +0.8 913,226 - mioC BNILDI_04610 0.53 -0.6 913,226 - mioC BNILDI_04610 0.53 +0.4 913,226 - mioC BNILDI_04610 0.53 -0.4 913,250 + mioC BNILDI_04610 0.59 +0.1 913,302 + mioC BNILDI_04610 0.70 -0.7 913,302 + mioC BNILDI_04610 0.70 -0.3 913,340 + mioC BNILDI_04610 0.79 -0.5 913,341 - mioC BNILDI_04610 0.79 -1.3 913,385 - mioC BNILDI_04610 0.89 +0.2 913,415 + +1.1 913,415 + -2.0 913,416 - -1.4 913,416 - +3.4 913,425 - +0.4 913,447 + -0.8 913,451 - -0.3 913,466 + -0.5 913,467 - -0.3 913,504 - +1.9 913,613 - asnC BNILDI_04615 0.20 -1.3 913,613 - asnC BNILDI_04615 0.20 +0.3 913,616 + asnC BNILDI_04615 0.20 +0.4 913,617 - asnC BNILDI_04615 0.20 -1.2 913,617 - asnC BNILDI_04615 0.20 -0.1 913,617 - asnC BNILDI_04615 0.20 -0.9 913,617 - asnC BNILDI_04615 0.20 +0.2 913,617 - asnC BNILDI_04615 0.20 +0.4 913,655 + asnC BNILDI_04615 0.29 -0.0 913,669 - asnC BNILDI_04615 0.32 -0.0 913,683 + asnC BNILDI_04615 0.35 +1.6 913,683 + asnC BNILDI_04615 0.35 +0.0 913,684 - asnC BNILDI_04615 0.35 +0.5 913,737 + asnC BNILDI_04615 0.47 -0.4 913,757 + asnC BNILDI_04615 0.51 -0.3 913,757 + asnC BNILDI_04615 0.51 -1.3 913,758 - asnC BNILDI_04615 0.51 +0.1 913,759 + asnC BNILDI_04615 0.51 +0.2 913,760 - asnC BNILDI_04615 0.52 +0.1 913,778 + asnC BNILDI_04615 0.56 +0.6 913,779 - asnC BNILDI_04615 0.56 +0.3 913,779 - asnC BNILDI_04615 0.56 +0.2 913,779 - asnC BNILDI_04615 0.56 +0.1 913,813 + asnC BNILDI_04615 0.63 -0.6 913,972 - -0.1 913,982 - -0.4 914,145 + -0.4 914,145 + +0.8 914,145 + -3.2 914,168 + -0.4 914,270 + asnA BNILDI_04620 0.14 +0.4 914,270 + asnA BNILDI_04620 0.14 -0.1 914,271 - asnA BNILDI_04620 0.14 -0.1 914,303 - asnA BNILDI_04620 0.17 -0.4 914,409 + asnA BNILDI_04620 0.28 +0.8 914,409 + asnA BNILDI_04620 0.28 +0.3 914,410 - asnA BNILDI_04620 0.28 -0.0 914,469 + asnA BNILDI_04620 0.34 +2.3 914,469 + asnA BNILDI_04620 0.34 +0.3 914,543 - asnA BNILDI_04620 0.41 -0.1 914,636 - asnA BNILDI_04620 0.51 -0.2 914,650 + asnA BNILDI_04620 0.52 +0.1 914,651 - asnA BNILDI_04620 0.52 -0.8 914,784 + asnA BNILDI_04620 0.66 +0.1 914,785 - asnA BNILDI_04620 0.66 -1.6 914,837 + asnA BNILDI_04620 0.71 +0.4 915,082 + -0.4 915,083 - -0.0 915,083 - -0.4 915,083 - +0.0 915,126 + +0.5 915,186 + -1.9 915,187 - +0.6 915,187 - -1.4 915,260 - -0.1 915,427 + viaA BNILDI_04625 0.20 -0.3 915,497 - viaA BNILDI_04625 0.25 -0.1 915,501 + viaA BNILDI_04625 0.26 -0.2 915,501 + viaA BNILDI_04625 0.26 +1.2 915,502 - viaA BNILDI_04625 0.26 +0.6 915,502 - viaA BNILDI_04625 0.26 +0.6 915,502 - viaA BNILDI_04625 0.26 +0.1 915,504 + viaA BNILDI_04625 0.26 -0.4 915,504 + viaA BNILDI_04625 0.26 +0.0 915,505 - viaA BNILDI_04625 0.26 +0.7 915,505 - viaA BNILDI_04625 0.26 +1.6 915,505 - viaA BNILDI_04625 0.26 +1.1 915,506 + viaA BNILDI_04625 0.26 +0.2 915,506 + viaA BNILDI_04625 0.26 -2.6 915,507 - viaA BNILDI_04625 0.26 +0.1 915,577 - viaA BNILDI_04625 0.31 +0.1 915,648 + viaA BNILDI_04625 0.36 -0.6 915,649 - viaA BNILDI_04625 0.36 -0.5 915,649 - viaA BNILDI_04625 0.36 +0.4 915,649 - viaA BNILDI_04625 0.36 -0.4 915,652 + viaA BNILDI_04625 0.36 +0.7 915,653 - viaA BNILDI_04625 0.36 -0.1 915,705 - viaA BNILDI_04625 0.40 +0.5 915,746 + viaA BNILDI_04625 0.42 -0.5 915,747 - viaA BNILDI_04625 0.42 -0.0 916,027 - viaA BNILDI_04625 0.62 +1.1 916,057 - viaA BNILDI_04625 0.64 -0.7 916,057 - viaA BNILDI_04625 0.64 +0.0 916,115 - viaA BNILDI_04625 0.68 -0.7
Or see this region's nucleotide sequence