Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malS and avtA are separated by 177 nucleotides avtA and hycB are separated by 150 nucleotides hycB and yiaJ are separated by 101 nucleotides
BNILDI_03680: malS - alpha-amylase, at 718,129 to 720,159
malS
BNILDI_03685: avtA - valine--pyruvate transaminase, at 720,337 to 721,590
avtA
BNILDI_03695: hycB - electron transporter, at 721,741 to 722,214
hycB
BNILDI_03700: yiaJ - IclR family transcriptional regulator YiaJ, at 722,316 to 723,164
yiaJ
Position (kb) 720
721
722 Strain fitness (log2 ratio) -2
-1
0
1
2 at 719.365 kb on - strand, within malS at 719.389 kb on + strand, within malS at 719.389 kb on + strand, within malS at 719.433 kb on + strand, within malS at 719.434 kb on - strand, within malS at 719.446 kb on + strand, within malS at 719.462 kb on + strand, within malS at 719.606 kb on + strand, within malS at 719.606 kb on + strand, within malS at 719.607 kb on - strand, within malS at 719.669 kb on - strand, within malS at 719.807 kb on - strand, within malS at 719.807 kb on - strand, within malS at 719.864 kb on + strand, within malS at 719.865 kb on - strand, within malS at 719.865 kb on - strand, within malS at 719.868 kb on - strand, within malS at 719.868 kb on - strand, within malS at 719.870 kb on + strand, within malS at 719.870 kb on + strand, within malS at 719.870 kb on + strand, within malS at 719.871 kb on - strand, within malS at 719.992 kb on - strand at 720.210 kb on - strand at 720.384 kb on + strand at 720.385 kb on - strand at 720.385 kb on - strand at 720.482 kb on - strand, within avtA at 720.545 kb on - strand, within avtA at 720.675 kb on - strand, within avtA at 720.675 kb on - strand, within avtA at 720.678 kb on + strand, within avtA at 720.678 kb on + strand, within avtA at 720.998 kb on - strand, within avtA at 721.153 kb on - strand, within avtA at 721.288 kb on - strand, within avtA at 721.311 kb on - strand, within avtA at 721.482 kb on + strand at 721.483 kb on - strand at 721.483 kb on - strand at 721.494 kb on + strand at 721.507 kb on + strand at 721.508 kb on - strand at 721.508 kb on - strand at 721.511 kb on + strand at 721.511 kb on + strand at 721.512 kb on - strand at 721.563 kb on - strand at 721.622 kb on + strand at 721.638 kb on + strand at 721.866 kb on + strand, within hycB at 721.867 kb on - strand, within hycB at 721.867 kb on - strand, within hycB at 722.073 kb on + strand, within hycB at 722.167 kb on - strand at 722.167 kb on - strand at 722.202 kb on + strand at 722.348 kb on + strand at 722.349 kb on - strand at 722.363 kb on + strand at 722.364 kb on - strand at 722.478 kb on - strand, within yiaJ at 722.480 kb on + strand, within yiaJ at 722.481 kb on - strand, within yiaJ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 719,365 - malS BNILDI_03680 0.61 +0.6 719,389 + malS BNILDI_03680 0.62 +0.3 719,389 + malS BNILDI_03680 0.62 +0.4 719,433 + malS BNILDI_03680 0.64 +0.1 719,434 - malS BNILDI_03680 0.64 +0.1 719,446 + malS BNILDI_03680 0.65 +0.2 719,462 + malS BNILDI_03680 0.66 +0.2 719,606 + malS BNILDI_03680 0.73 -0.1 719,606 + malS BNILDI_03680 0.73 +0.1 719,607 - malS BNILDI_03680 0.73 -1.1 719,669 - malS BNILDI_03680 0.76 +0.3 719,807 - malS BNILDI_03680 0.83 -1.2 719,807 - malS BNILDI_03680 0.83 +0.0 719,864 + malS BNILDI_03680 0.85 +0.3 719,865 - malS BNILDI_03680 0.85 -0.6 719,865 - malS BNILDI_03680 0.85 +1.8 719,868 - malS BNILDI_03680 0.86 +0.6 719,868 - malS BNILDI_03680 0.86 +0.6 719,870 + malS BNILDI_03680 0.86 -2.1 719,870 + malS BNILDI_03680 0.86 -0.8 719,870 + malS BNILDI_03680 0.86 +0.6 719,871 - malS BNILDI_03680 0.86 +0.1 719,992 - +0.9 720,210 - +0.1 720,384 + +0.5 720,385 - +1.3 720,385 - -0.2 720,482 - avtA BNILDI_03685 0.12 -0.1 720,545 - avtA BNILDI_03685 0.17 -1.7 720,675 - avtA BNILDI_03685 0.27 -0.6 720,675 - avtA BNILDI_03685 0.27 -0.6 720,678 + avtA BNILDI_03685 0.27 -0.3 720,678 + avtA BNILDI_03685 0.27 -0.3 720,998 - avtA BNILDI_03685 0.53 +0.3 721,153 - avtA BNILDI_03685 0.65 +0.1 721,288 - avtA BNILDI_03685 0.76 +0.8 721,311 - avtA BNILDI_03685 0.78 -0.4 721,482 + -0.2 721,483 - -1.2 721,483 - +0.2 721,494 + -0.1 721,507 + -0.1 721,508 - -0.3 721,508 - -0.3 721,511 + -0.4 721,511 + +0.1 721,512 - +1.0 721,563 - -0.3 721,622 + +0.2 721,638 + +1.0 721,866 + hycB BNILDI_03695 0.26 +0.4 721,867 - hycB BNILDI_03695 0.27 -1.4 721,867 - hycB BNILDI_03695 0.27 -0.8 722,073 + hycB BNILDI_03695 0.70 +0.0 722,167 - +0.7 722,167 - -0.2 722,202 + -0.8 722,348 + +0.2 722,349 - +0.9 722,363 + +1.7 722,364 - +0.3 722,478 - yiaJ BNILDI_03700 0.19 +0.1 722,480 + yiaJ BNILDI_03700 0.19 -1.2 722,481 - yiaJ BNILDI_03700 0.19 -1.3
Or see this region's nucleotide sequence