Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt igaA and yrfG are separated by 64 nucleotides yrfG and hslR are separated by 10 nucleotides hslR and hslO are separated by 24 nucleotides
BNILDI_02660: igaA - intracellular growth attenuator protein IgaA, at 503,206 to 505,341
igaA
BNILDI_02665: yrfG - GMP/IMP nucleotidase, at 505,406 to 506,074
yrfG
BNILDI_02670: hslR - ribosome-associated heat shock protein Hsp15, at 506,085 to 506,486
hslR
BNILDI_02675: hslO - Hsp33 family molecular chaperone HslO, at 506,511 to 507,389
hslO
Position (kb) 506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 505.361 kb on - strand at 505.411 kb on + strand at 505.434 kb on + strand at 505.434 kb on + strand at 505.434 kb on + strand at 505.434 kb on + strand at 505.435 kb on - strand at 505.435 kb on - strand at 505.486 kb on + strand, within yrfG at 505.490 kb on + strand, within yrfG at 505.490 kb on + strand, within yrfG at 505.491 kb on - strand, within yrfG at 505.562 kb on + strand, within yrfG at 505.563 kb on - strand, within yrfG at 505.564 kb on + strand, within yrfG at 505.564 kb on + strand, within yrfG at 505.564 kb on + strand, within yrfG at 505.565 kb on - strand, within yrfG at 505.565 kb on - strand, within yrfG at 505.594 kb on + strand, within yrfG at 505.594 kb on + strand, within yrfG at 505.599 kb on + strand, within yrfG at 505.793 kb on - strand, within yrfG at 505.822 kb on - strand, within yrfG at 505.918 kb on + strand, within yrfG at 505.987 kb on + strand, within yrfG at 506.045 kb on + strand at 506.287 kb on + strand, within hslR at 506.288 kb on - strand, within hslR at 506.337 kb on + strand, within hslR at 506.337 kb on + strand, within hslR at 506.396 kb on + strand, within hslR at 506.397 kb on - strand, within hslR at 506.484 kb on + strand at 506.484 kb on + strand at 506.484 kb on + strand at 506.485 kb on - strand at 506.510 kb on + strand at 506.883 kb on + strand, within hslO at 506.976 kb on - strand, within hslO at 506.992 kb on + strand, within hslO at 506.992 kb on + strand, within hslO at 507.018 kb on - strand, within hslO at 507.031 kb on + strand, within hslO at 507.318 kb on + strand at 507.394 kb on + strand at 507.394 kb on + strand at 507.395 kb on - strand at 507.395 kb on - strand at 507.432 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 505,361 - -1.9 505,411 + +0.6 505,434 + -3.2 505,434 + +1.0 505,434 + +1.1 505,434 + +0.4 505,435 - -0.2 505,435 - +0.9 505,486 + yrfG BNILDI_02665 0.12 -0.1 505,490 + yrfG BNILDI_02665 0.13 +0.4 505,490 + yrfG BNILDI_02665 0.13 -1.5 505,491 - yrfG BNILDI_02665 0.13 +0.6 505,562 + yrfG BNILDI_02665 0.23 +0.4 505,563 - yrfG BNILDI_02665 0.23 +1.1 505,564 + yrfG BNILDI_02665 0.24 +0.4 505,564 + yrfG BNILDI_02665 0.24 -0.1 505,564 + yrfG BNILDI_02665 0.24 -0.1 505,565 - yrfG BNILDI_02665 0.24 -0.9 505,565 - yrfG BNILDI_02665 0.24 -0.0 505,594 + yrfG BNILDI_02665 0.28 +0.8 505,594 + yrfG BNILDI_02665 0.28 -0.8 505,599 + yrfG BNILDI_02665 0.29 -0.2 505,793 - yrfG BNILDI_02665 0.58 +0.4 505,822 - yrfG BNILDI_02665 0.62 -1.2 505,918 + yrfG BNILDI_02665 0.77 -0.7 505,987 + yrfG BNILDI_02665 0.87 +0.2 506,045 + -0.1 506,287 + hslR BNILDI_02670 0.50 +0.4 506,288 - hslR BNILDI_02670 0.50 -0.0 506,337 + hslR BNILDI_02670 0.63 -1.9 506,337 + hslR BNILDI_02670 0.63 -0.4 506,396 + hslR BNILDI_02670 0.77 +0.6 506,397 - hslR BNILDI_02670 0.78 -2.2 506,484 + +1.1 506,484 + -0.4 506,484 + +0.4 506,485 - -0.2 506,510 + -1.2 506,883 + hslO BNILDI_02675 0.42 -1.1 506,976 - hslO BNILDI_02675 0.53 +0.1 506,992 + hslO BNILDI_02675 0.55 -2.1 506,992 + hslO BNILDI_02675 0.55 +0.7 507,018 - hslO BNILDI_02675 0.58 -3.0 507,031 + hslO BNILDI_02675 0.59 +0.8 507,318 + -0.3 507,394 + +0.6 507,394 + +0.2 507,395 - -1.6 507,395 - +0.2 507,432 + -1.2
Or see this region's nucleotide sequence