Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkA and mscL are separated by 129 nucleotides mscL and arfA overlap by 4 nucleotides arfA and zntR are separated by 55 nucleotides zntR and yhdN are separated by 10 nucleotides yhdN and BNILDI_02230 are separated by 106 nucleotides
BNILDI_02205: trkA - Trk system potassium transporter TrkA, at 433,595 to 434,971
trkA
BNILDI_02210: mscL - large-conductance mechanosensitive channel protein MscL, at 435,101 to 435,511
mscL
BNILDI_02215: arfA - alternative ribosome-rescue factor ArfA, at 435,508 to 435,726
arfA
BNILDI_02220: zntR - Zn(2+)-responsive transcriptional regulator, at 435,782 to 436,207
zntR
BNILDI_02225: yhdN - Uncharacterized protein YhdN, at 436,218 to 436,586
yhdN
BNILDI_02230: BNILDI_02230 - 50S ribosomal protein L17, at 436,693 to 437,076
_02230
Position (kb) 435
436 Strain fitness (log2 ratio) -2
-1
0
1
2 at 434.969 kb on + strand at 434.974 kb on - strand at 434.976 kb on + strand at 435.066 kb on - strand at 435.066 kb on - strand at 435.072 kb on - strand at 435.199 kb on - strand, within mscL at 435.388 kb on + strand, within mscL at 435.388 kb on + strand, within mscL at 435.448 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.462 kb on + strand, within mscL at 435.509 kb on + strand at 435.509 kb on + strand at 435.510 kb on - strand at 435.612 kb on - strand, within arfA at 435.612 kb on - strand, within arfA at 435.671 kb on - strand, within arfA at 435.680 kb on - strand, within arfA at 435.705 kb on + strand at 435.705 kb on + strand at 435.706 kb on - strand at 435.709 kb on - strand at 435.726 kb on + strand at 435.840 kb on + strand, within zntR at 435.841 kb on - strand, within zntR at 435.901 kb on + strand, within zntR at 435.901 kb on + strand, within zntR at 435.976 kb on + strand, within zntR at 436.037 kb on + strand, within zntR at 436.037 kb on + strand, within zntR at 436.037 kb on + strand, within zntR at 436.037 kb on + strand, within zntR at 436.037 kb on + strand, within zntR at 436.150 kb on - strand, within zntR at 436.207 kb on + strand at 436.207 kb on + strand at 436.208 kb on - strand at 436.208 kb on - strand at 436.208 kb on - strand at 436.212 kb on - strand at 436.219 kb on + strand at 436.458 kb on + strand, within yhdN at 436.459 kb on - strand, within yhdN at 436.521 kb on - strand, within yhdN at 436.552 kb on - strand at 436.552 kb on - strand at 436.579 kb on - strand at 436.586 kb on + strand at 436.586 kb on + strand at 436.591 kb on - strand at 436.615 kb on + strand at 436.621 kb on + strand at 436.621 kb on + strand at 436.627 kb on - strand at 436.630 kb on + strand at 436.630 kb on + strand at 436.630 kb on + strand at 436.631 kb on - strand at 436.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 434,969 + +1.5 434,974 - +1.4 434,976 + +1.0 435,066 - +0.7 435,066 - +0.5 435,072 - -1.1 435,199 - mscL BNILDI_02210 0.24 +0.5 435,388 + mscL BNILDI_02210 0.70 +1.3 435,388 + mscL BNILDI_02210 0.70 -1.5 435,448 + mscL BNILDI_02210 0.84 -1.0 435,459 + mscL BNILDI_02210 0.87 +0.7 435,459 + mscL BNILDI_02210 0.87 +0.1 435,459 + mscL BNILDI_02210 0.87 +0.5 435,462 + mscL BNILDI_02210 0.88 +0.8 435,509 + +0.1 435,509 + +0.7 435,510 - +0.2 435,612 - arfA BNILDI_02215 0.47 +0.7 435,612 - arfA BNILDI_02215 0.47 -1.3 435,671 - arfA BNILDI_02215 0.74 -0.0 435,680 - arfA BNILDI_02215 0.79 -2.0 435,705 + +2.2 435,705 + -1.1 435,706 - -0.3 435,709 - +0.5 435,726 + -2.5 435,840 + zntR BNILDI_02220 0.14 -0.2 435,841 - zntR BNILDI_02220 0.14 +0.1 435,901 + zntR BNILDI_02220 0.28 +0.5 435,901 + zntR BNILDI_02220 0.28 -0.1 435,976 + zntR BNILDI_02220 0.46 -1.9 436,037 + zntR BNILDI_02220 0.60 -0.2 436,037 + zntR BNILDI_02220 0.60 +0.3 436,037 + zntR BNILDI_02220 0.60 +0.6 436,037 + zntR BNILDI_02220 0.60 -1.4 436,037 + zntR BNILDI_02220 0.60 +0.9 436,150 - zntR BNILDI_02220 0.86 -0.9 436,207 + +1.1 436,207 + -0.1 436,208 - -1.9 436,208 - +0.7 436,208 - -0.0 436,212 - -0.2 436,219 + +0.1 436,458 + yhdN BNILDI_02225 0.65 -0.4 436,459 - yhdN BNILDI_02225 0.65 -0.3 436,521 - yhdN BNILDI_02225 0.82 -0.1 436,552 - +0.3 436,552 - +0.2 436,579 - +0.6 436,586 + -0.0 436,586 + -0.0 436,591 - -2.5 436,615 + -0.2 436,621 + -1.7 436,621 + -0.9 436,627 - +0.7 436,630 + +0.6 436,630 + -1.0 436,630 + +0.2 436,631 - +0.2 436,631 - +0.3
Or see this region's nucleotide sequence