Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoD and mug are separated by 77 nucleotides mug and Ile2_trna are separated by 124 nucleotides Ile2_trna and nfeF are separated by 53 nucleotides
BNILDI_01070: rpoD - RNA polymerase sigma factor RpoD, at 206,690 to 208,531
rpoD
BNILDI_01075: mug - G/U mismatch-specific DNA glycosylase, at 208,609 to 209,115
mug
BNILDI_01080: Ile2_trna - tRNA-Ile2(cat), at 209,240 to 209,315
_trna
BNILDI_01085: nfeF - NADPH-dependent ferric chelate reductase, at 209,369 to 210,133
nfeF
Position (kb) 208
209
210 Strain fitness (log2 ratio) -1
0
1
2 at 208.685 kb on - strand, within mug at 208.781 kb on + strand, within mug at 208.781 kb on + strand, within mug at 208.906 kb on + strand, within mug at 208.991 kb on + strand, within mug at 208.991 kb on + strand, within mug at 208.991 kb on + strand, within mug at 208.992 kb on - strand, within mug at 208.992 kb on - strand, within mug at 208.992 kb on - strand, within mug at 208.993 kb on + strand, within mug at 208.993 kb on + strand, within mug at 208.993 kb on + strand, within mug at 208.993 kb on + strand, within mug at 209.116 kb on - strand at 209.119 kb on + strand at 209.172 kb on + strand at 209.320 kb on + strand at 209.320 kb on + strand at 209.320 kb on + strand at 209.320 kb on + strand at 209.321 kb on - strand at 209.458 kb on + strand, within nfeF at 209.484 kb on + strand, within nfeF at 209.484 kb on + strand, within nfeF at 209.484 kb on + strand, within nfeF at 209.485 kb on - strand, within nfeF at 209.485 kb on - strand, within nfeF at 209.525 kb on + strand, within nfeF at 209.560 kb on + strand, within nfeF at 209.560 kb on + strand, within nfeF at 209.561 kb on - strand, within nfeF at 209.584 kb on - strand, within nfeF at 209.703 kb on + strand, within nfeF at 209.703 kb on + strand, within nfeF at 209.719 kb on - strand, within nfeF at 209.869 kb on + strand, within nfeF at 209.869 kb on + strand, within nfeF at 209.872 kb on - strand, within nfeF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 208,685 - mug BNILDI_01075 0.15 -0.0 208,781 + mug BNILDI_01075 0.34 +0.4 208,781 + mug BNILDI_01075 0.34 +0.0 208,906 + mug BNILDI_01075 0.59 +0.3 208,991 + mug BNILDI_01075 0.75 -1.3 208,991 + mug BNILDI_01075 0.75 +0.5 208,991 + mug BNILDI_01075 0.75 +0.7 208,992 - mug BNILDI_01075 0.76 +0.3 208,992 - mug BNILDI_01075 0.76 -0.3 208,992 - mug BNILDI_01075 0.76 +0.7 208,993 + mug BNILDI_01075 0.76 -1.3 208,993 + mug BNILDI_01075 0.76 -1.6 208,993 + mug BNILDI_01075 0.76 -0.0 208,993 + mug BNILDI_01075 0.76 -1.2 209,116 - -0.9 209,119 + -1.0 209,172 + +1.0 209,320 + +1.0 209,320 + -0.2 209,320 + -0.6 209,320 + -0.1 209,321 - +2.4 209,458 + nfeF BNILDI_01085 0.12 -0.3 209,484 + nfeF BNILDI_01085 0.15 -1.4 209,484 + nfeF BNILDI_01085 0.15 -0.1 209,484 + nfeF BNILDI_01085 0.15 +0.1 209,485 - nfeF BNILDI_01085 0.15 -0.0 209,485 - nfeF BNILDI_01085 0.15 +1.2 209,525 + nfeF BNILDI_01085 0.20 -0.3 209,560 + nfeF BNILDI_01085 0.25 -1.2 209,560 + nfeF BNILDI_01085 0.25 +0.2 209,561 - nfeF BNILDI_01085 0.25 -1.3 209,584 - nfeF BNILDI_01085 0.28 -0.1 209,703 + nfeF BNILDI_01085 0.44 +0.1 209,703 + nfeF BNILDI_01085 0.44 +0.1 209,719 - nfeF BNILDI_01085 0.46 +0.0 209,869 + nfeF BNILDI_01085 0.65 +1.0 209,869 + nfeF BNILDI_01085 0.65 -0.3 209,872 - nfeF BNILDI_01085 0.66 +0.9
Or see this region's nucleotide sequence