Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqhD and dkgA are separated by 104 nucleotides dkgA and yqhG are separated by 199 nucleotides
BNILDI_00795: yqhD - alcohol dehydrogenase, at 152,586 to 153,749
yqhD
BNILDI_00805: dkgA - 2,5-didehydrogluconate reductase DkgA, at 153,854 to 154,681
dkgA
BNILDI_00810: yqhG - Uncharacterized protein YqhG, at 154,881 to 155,807
yqhG
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 152.913 kb on + strand, within yqhD at 152.966 kb on + strand, within yqhD at 153.047 kb on + strand, within yqhD at 153.097 kb on + strand, within yqhD at 153.120 kb on + strand, within yqhD at 153.121 kb on - strand, within yqhD at 153.121 kb on - strand, within yqhD at 153.127 kb on - strand, within yqhD at 153.192 kb on + strand, within yqhD at 153.193 kb on - strand, within yqhD at 153.193 kb on - strand, within yqhD at 153.193 kb on - strand, within yqhD at 153.491 kb on + strand, within yqhD at 153.504 kb on + strand, within yqhD at 153.552 kb on - strand, within yqhD at 153.595 kb on + strand, within yqhD at 153.728 kb on + strand at 153.729 kb on - strand at 153.729 kb on - strand at 153.730 kb on + strand at 153.730 kb on + strand at 153.731 kb on - strand at 153.733 kb on - strand at 153.796 kb on + strand at 153.796 kb on + strand at 153.797 kb on - strand at 153.838 kb on + strand at 153.838 kb on + strand at 153.840 kb on + strand at 153.840 kb on + strand at 153.841 kb on - strand at 153.841 kb on - strand at 153.841 kb on - strand at 153.841 kb on - strand at 153.853 kb on + strand at 153.854 kb on - strand at 153.874 kb on + strand at 153.874 kb on + strand at 153.931 kb on + strand at 153.942 kb on + strand, within dkgA at 154.005 kb on - strand, within dkgA at 154.006 kb on + strand, within dkgA at 154.078 kb on + strand, within dkgA at 154.154 kb on - strand, within dkgA at 154.167 kb on + strand, within dkgA at 154.168 kb on - strand, within dkgA at 154.168 kb on - strand, within dkgA at 154.168 kb on - strand, within dkgA at 154.168 kb on - strand, within dkgA at 154.199 kb on + strand, within dkgA at 154.199 kb on + strand, within dkgA at 154.199 kb on + strand, within dkgA at 154.327 kb on - strand, within dkgA at 154.327 kb on - strand, within dkgA at 154.475 kb on - strand, within dkgA at 154.564 kb on + strand, within dkgA at 154.564 kb on + strand, within dkgA at 154.793 kb on - strand at 154.793 kb on - strand at 154.793 kb on - strand at 154.794 kb on + strand at 154.795 kb on - strand at 154.826 kb on + strand at 154.827 kb on - strand at 154.843 kb on + strand at 154.844 kb on - strand at 154.844 kb on - strand at 154.888 kb on + strand at 154.892 kb on - strand at 154.892 kb on - strand at 154.954 kb on + strand at 154.954 kb on + strand at 154.955 kb on - strand at 154.977 kb on + strand, within yqhG at 154.977 kb on + strand, within yqhG at 154.978 kb on - strand, within yqhG at 154.978 kb on - strand, within yqhG at 154.988 kb on + strand, within yqhG at 154.989 kb on - strand, within yqhG at 154.989 kb on - strand, within yqhG at 155.078 kb on + strand, within yqhG at 155.100 kb on - strand, within yqhG at 155.235 kb on - strand, within yqhG at 155.324 kb on - strand, within yqhG at 155.324 kb on - strand, within yqhG at 155.324 kb on - strand, within yqhG at 155.333 kb on + strand, within yqhG at 155.420 kb on + strand, within yqhG at 155.421 kb on - strand, within yqhG at 155.491 kb on - strand, within yqhG at 155.556 kb on + strand, within yqhG at 155.661 kb on - strand, within yqhG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 152,913 + yqhD BNILDI_00795 0.28 -2.1 152,966 + yqhD BNILDI_00795 0.33 +0.9 153,047 + yqhD BNILDI_00795 0.40 +0.8 153,097 + yqhD BNILDI_00795 0.44 +0.4 153,120 + yqhD BNILDI_00795 0.46 +0.4 153,121 - yqhD BNILDI_00795 0.46 -1.6 153,121 - yqhD BNILDI_00795 0.46 +0.3 153,127 - yqhD BNILDI_00795 0.46 +0.7 153,192 + yqhD BNILDI_00795 0.52 -0.4 153,193 - yqhD BNILDI_00795 0.52 -0.9 153,193 - yqhD BNILDI_00795 0.52 +0.2 153,193 - yqhD BNILDI_00795 0.52 +0.8 153,491 + yqhD BNILDI_00795 0.78 -0.4 153,504 + yqhD BNILDI_00795 0.79 +1.1 153,552 - yqhD BNILDI_00795 0.83 -0.5 153,595 + yqhD BNILDI_00795 0.87 +1.7 153,728 + -0.2 153,729 - +0.7 153,729 - +0.6 153,730 + -0.9 153,730 + +1.3 153,731 - -1.2 153,733 - +0.2 153,796 + -0.3 153,796 + -1.0 153,797 - -1.0 153,838 + +1.3 153,838 + -1.4 153,840 + +0.0 153,840 + +0.0 153,841 - -0.4 153,841 - -0.3 153,841 - +0.5 153,841 - -1.1 153,853 + -0.1 153,854 - +0.3 153,874 + -0.5 153,874 + +0.2 153,931 + -1.4 153,942 + dkgA BNILDI_00805 0.11 -0.3 154,005 - dkgA BNILDI_00805 0.18 -0.0 154,006 + dkgA BNILDI_00805 0.18 +0.4 154,078 + dkgA BNILDI_00805 0.27 -0.7 154,154 - dkgA BNILDI_00805 0.36 -0.2 154,167 + dkgA BNILDI_00805 0.38 +0.2 154,168 - dkgA BNILDI_00805 0.38 -0.3 154,168 - dkgA BNILDI_00805 0.38 -0.4 154,168 - dkgA BNILDI_00805 0.38 -0.1 154,168 - dkgA BNILDI_00805 0.38 +0.1 154,199 + dkgA BNILDI_00805 0.42 -0.3 154,199 + dkgA BNILDI_00805 0.42 -0.6 154,199 + dkgA BNILDI_00805 0.42 -0.2 154,327 - dkgA BNILDI_00805 0.57 -0.2 154,327 - dkgA BNILDI_00805 0.57 -0.8 154,475 - dkgA BNILDI_00805 0.75 +0.3 154,564 + dkgA BNILDI_00805 0.86 +2.5 154,564 + dkgA BNILDI_00805 0.86 +0.7 154,793 - +2.0 154,793 - -1.8 154,793 - +1.0 154,794 + -1.2 154,795 - -0.6 154,826 + +1.0 154,827 - +0.8 154,843 + +1.1 154,844 - +0.0 154,844 - +0.3 154,888 + -0.1 154,892 - +0.3 154,892 - +0.2 154,954 + -0.4 154,954 + -0.2 154,955 - -0.1 154,977 + yqhG BNILDI_00810 0.10 -0.7 154,977 + yqhG BNILDI_00810 0.10 +0.2 154,978 - yqhG BNILDI_00810 0.10 -0.0 154,978 - yqhG BNILDI_00810 0.10 +1.4 154,988 + yqhG BNILDI_00810 0.12 -0.2 154,989 - yqhG BNILDI_00810 0.12 -0.0 154,989 - yqhG BNILDI_00810 0.12 +0.0 155,078 + yqhG BNILDI_00810 0.21 +1.1 155,100 - yqhG BNILDI_00810 0.24 -2.5 155,235 - yqhG BNILDI_00810 0.38 +0.1 155,324 - yqhG BNILDI_00810 0.48 +0.2 155,324 - yqhG BNILDI_00810 0.48 -0.4 155,324 - yqhG BNILDI_00810 0.48 -0.6 155,333 + yqhG BNILDI_00810 0.49 -0.3 155,420 + yqhG BNILDI_00810 0.58 +0.5 155,421 - yqhG BNILDI_00810 0.58 -1.3 155,491 - yqhG BNILDI_00810 0.66 +0.8 155,556 + yqhG BNILDI_00810 0.73 -0.4 155,661 - yqhG BNILDI_00810 0.84 +0.0
Or see this region's nucleotide sequence