Strain Fitness in Escherichia coli ECOR38 around HEPCGN_19275

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlgt and thyA are separated by 6 nucleotidesthyA and ppdA are separated by 184 nucleotidesppdA and pulJ overlap by 10 nucleotidespulJ and ygdB overlap by 4 nucleotidesygdB and ppdC overlap by 16 nucleotides HEPCGN_19265: lgt - prolipoprotein diacylglyceryl transferase, at 2,604,375 to 2,605,250 lgt HEPCGN_19270: thyA - thymidylate synthase, at 2,605,257 to 2,606,051 thyA HEPCGN_19275: ppdA - prepilin peptidase-dependent protein, at 2,606,236 to 2,606,706 ppdA HEPCGN_19280: pulJ - prepilin peptidase-dependent protein, at 2,606,697 to 2,607,260 pulJ HEPCGN_19285: ygdB - DUF2509 domain-containing protein, at 2,607,257 to 2,607,664 ygdB HEPCGN_19290: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,607,649 to 2,607,972 ppdC Position (kb) 2606 2607Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2605.892 kb on + strand, within thyAat 2606.049 kb on + strandat 2606.050 kb on - strandat 2606.050 kb on - strandat 2606.050 kb on - strandat 2606.053 kb on + strandat 2606.164 kb on + strandat 2606.165 kb on - strandat 2606.165 kb on - strandat 2606.226 kb on + strandat 2606.226 kb on + strandat 2606.256 kb on + strandat 2606.301 kb on - strand, within ppdAat 2606.301 kb on - strand, within ppdAat 2606.430 kb on + strand, within ppdAat 2606.430 kb on + strand, within ppdAat 2606.431 kb on - strand, within ppdAat 2606.761 kb on + strand, within pulJat 2606.761 kb on + strand, within pulJat 2606.762 kb on - strand, within pulJat 2606.762 kb on - strand, within pulJat 2606.797 kb on + strand, within pulJat 2606.798 kb on - strand, within pulJat 2606.825 kb on + strand, within pulJat 2606.895 kb on + strand, within pulJat 2607.185 kb on + strand, within pulJat 2607.186 kb on - strand, within pulJat 2607.186 kb on - strand, within pulJat 2607.298 kb on + strand, within ygdBat 2607.325 kb on - strand, within ygdBat 2607.476 kb on + strand, within ygdBat 2607.510 kb on + strand, within ygdBat 2607.510 kb on + strand, within ygdBat 2607.532 kb on - strand, within ygdB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,605,892 + thyA HEPCGN_19270 0.80 -0.6
2,606,049 + -0.7
2,606,050 - -4.4
2,606,050 - -0.0
2,606,050 - -0.7
2,606,053 + -0.9
2,606,164 + +1.2
2,606,165 - +0.1
2,606,165 - +0.6
2,606,226 + +1.0
2,606,226 + +0.7
2,606,256 + -0.2
2,606,301 - ppdA HEPCGN_19275 0.14 -1.3
2,606,301 - ppdA HEPCGN_19275 0.14 -0.6
2,606,430 + ppdA HEPCGN_19275 0.41 +1.2
2,606,430 + ppdA HEPCGN_19275 0.41 -0.4
2,606,431 - ppdA HEPCGN_19275 0.41 +0.1
2,606,761 + pulJ HEPCGN_19280 0.11 -0.4
2,606,761 + pulJ HEPCGN_19280 0.11 +0.3
2,606,762 - pulJ HEPCGN_19280 0.12 -0.8
2,606,762 - pulJ HEPCGN_19280 0.12 +0.3
2,606,797 + pulJ HEPCGN_19280 0.18 -0.3
2,606,798 - pulJ HEPCGN_19280 0.18 +0.1
2,606,825 + pulJ HEPCGN_19280 0.23 -0.1
2,606,895 + pulJ HEPCGN_19280 0.35 +0.2
2,607,185 + pulJ HEPCGN_19280 0.87 -0.4
2,607,186 - pulJ HEPCGN_19280 0.87 +0.7
2,607,186 - pulJ HEPCGN_19280 0.87 +0.5
2,607,298 + ygdB HEPCGN_19285 0.10 +0.1
2,607,325 - ygdB HEPCGN_19285 0.17 +1.4
2,607,476 + ygdB HEPCGN_19285 0.54 +0.4
2,607,510 + ygdB HEPCGN_19285 0.62 -1.3
2,607,510 + ygdB HEPCGN_19285 0.62 -1.6
2,607,532 - ygdB HEPCGN_19285 0.67 +0.7

Or see this region's nucleotide sequence