Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygdR and ygdQ are separated by 137 nucleotides ygdQ and mutH are separated by 68 nucleotides mutH and ygdT are separated by 185 nucleotides ygdT and rppH are separated by 352 nucleotides
HEPCGN_19235: ygdR - lipoprotein YgdR, at 2,598,923 to 2,599,141
ygdR
HEPCGN_19240: ygdQ - UPF0053 inner membrane protein YgdQ, at 2,599,279 to 2,599,992
ygdQ
HEPCGN_19245: mutH - DNA mismatch repair endonuclease MutH, at 2,600,061 to 2,600,750
mutH
HEPCGN_19250: ygdT - Protein YgdT, at 2,600,936 to 2,601,082
ygdT
HEPCGN_19255: rppH - RNA pyrophosphohydrolase, at 2,601,435 to 2,601,965
rppH
Position (kb)
2600
2601 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2599.072 kb on - strand, within ygdR at 2599.072 kb on - strand, within ygdR at 2599.172 kb on - strand at 2599.175 kb on - strand at 2599.197 kb on - strand at 2599.198 kb on + strand at 2599.199 kb on - strand at 2599.199 kb on - strand at 2599.199 kb on - strand at 2599.199 kb on - strand at 2599.200 kb on + strand at 2599.248 kb on + strand at 2599.271 kb on - strand at 2599.349 kb on + strand at 2599.350 kb on - strand at 2599.355 kb on + strand, within ygdQ at 2599.524 kb on + strand, within ygdQ at 2599.525 kb on - strand, within ygdQ at 2599.525 kb on - strand, within ygdQ at 2599.581 kb on + strand, within ygdQ at 2599.581 kb on + strand, within ygdQ at 2599.581 kb on + strand, within ygdQ at 2599.582 kb on - strand, within ygdQ at 2599.582 kb on - strand, within ygdQ at 2599.695 kb on + strand, within ygdQ at 2599.753 kb on + strand, within ygdQ at 2599.819 kb on - strand, within ygdQ at 2599.902 kb on + strand, within ygdQ at 2599.966 kb on - strand at 2599.987 kb on + strand at 2599.987 kb on + strand at 2599.988 kb on - strand at 2599.995 kb on - strand at 2600.027 kb on + strand at 2600.027 kb on + strand at 2600.028 kb on - strand at 2600.028 kb on - strand at 2600.029 kb on + strand at 2600.030 kb on - strand at 2600.116 kb on + strand at 2600.208 kb on + strand, within mutH at 2600.209 kb on - strand, within mutH at 2600.262 kb on - strand, within mutH at 2600.363 kb on + strand, within mutH at 2600.363 kb on + strand, within mutH at 2600.364 kb on - strand, within mutH at 2600.364 kb on - strand, within mutH at 2600.364 kb on - strand, within mutH at 2600.364 kb on - strand, within mutH at 2600.441 kb on + strand, within mutH at 2600.441 kb on + strand, within mutH at 2600.442 kb on - strand, within mutH at 2600.444 kb on + strand, within mutH at 2600.555 kb on + strand, within mutH at 2600.556 kb on - strand, within mutH at 2600.669 kb on + strand, within mutH at 2600.670 kb on - strand, within mutH at 2600.698 kb on - strand at 2600.786 kb on - strand at 2600.844 kb on - strand at 2600.992 kb on + strand, within ygdT at 2601.320 kb on - strand at 2601.351 kb on + strand at 2601.351 kb on + strand at 2601.406 kb on + strand at 2601.407 kb on - strand at 2601.407 kb on - strand at 2601.408 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.410 kb on + strand at 2601.418 kb on + strand at 2601.429 kb on - strand at 2601.467 kb on - strand at 2601.470 kb on + strand at 2601.471 kb on - strand at 2601.585 kb on + strand, within rppH at 2601.613 kb on + strand, within rppH at 2601.614 kb on - strand, within rppH at 2601.664 kb on - strand, within rppH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,599,072 - ygdR HEPCGN_19235 0.68 -4.2 2,599,072 - ygdR HEPCGN_19235 0.68 -0.3 2,599,172 - +1.1 2,599,175 - +1.0 2,599,197 - -0.1 2,599,198 + -0.3 2,599,199 - -0.4 2,599,199 - -2.9 2,599,199 - +0.3 2,599,199 - +0.1 2,599,200 + -0.2 2,599,248 + -1.3 2,599,271 - -3.3 2,599,349 + +1.4 2,599,350 - -0.4 2,599,355 + ygdQ HEPCGN_19240 0.11 +0.5 2,599,524 + ygdQ HEPCGN_19240 0.34 +0.5 2,599,525 - ygdQ HEPCGN_19240 0.34 +0.4 2,599,525 - ygdQ HEPCGN_19240 0.34 -3.2 2,599,581 + ygdQ HEPCGN_19240 0.42 +1.1 2,599,581 + ygdQ HEPCGN_19240 0.42 -0.9 2,599,581 + ygdQ HEPCGN_19240 0.42 +0.3 2,599,582 - ygdQ HEPCGN_19240 0.42 +0.5 2,599,582 - ygdQ HEPCGN_19240 0.42 -0.5 2,599,695 + ygdQ HEPCGN_19240 0.58 -0.7 2,599,753 + ygdQ HEPCGN_19240 0.66 +1.2 2,599,819 - ygdQ HEPCGN_19240 0.76 -0.8 2,599,902 + ygdQ HEPCGN_19240 0.87 +0.4 2,599,966 - -0.5 2,599,987 + +1.3 2,599,987 + +0.8 2,599,988 - +0.2 2,599,995 - -1.3 2,600,027 + -0.9 2,600,027 + -1.9 2,600,028 - +0.4 2,600,028 - +0.0 2,600,029 + +0.2 2,600,030 - -0.2 2,600,116 + +0.0 2,600,208 + mutH HEPCGN_19245 0.21 +1.6 2,600,209 - mutH HEPCGN_19245 0.21 -0.2 2,600,262 - mutH HEPCGN_19245 0.29 -1.4 2,600,363 + mutH HEPCGN_19245 0.44 +0.3 2,600,363 + mutH HEPCGN_19245 0.44 -0.5 2,600,364 - mutH HEPCGN_19245 0.44 -0.2 2,600,364 - mutH HEPCGN_19245 0.44 -1.0 2,600,364 - mutH HEPCGN_19245 0.44 -0.1 2,600,364 - mutH HEPCGN_19245 0.44 -2.6 2,600,441 + mutH HEPCGN_19245 0.55 -0.3 2,600,441 + mutH HEPCGN_19245 0.55 +0.7 2,600,442 - mutH HEPCGN_19245 0.55 -0.5 2,600,444 + mutH HEPCGN_19245 0.56 -0.1 2,600,555 + mutH HEPCGN_19245 0.72 -0.9 2,600,556 - mutH HEPCGN_19245 0.72 -2.6 2,600,669 + mutH HEPCGN_19245 0.88 +1.1 2,600,670 - mutH HEPCGN_19245 0.88 +0.5 2,600,698 - -0.5 2,600,786 - +1.8 2,600,844 - +0.7 2,600,992 + ygdT HEPCGN_19250 0.38 -0.2 2,601,320 - -0.9 2,601,351 + -0.3 2,601,351 + +1.1 2,601,406 + -0.9 2,601,407 - -1.0 2,601,407 - -0.3 2,601,408 + +0.6 2,601,410 + -0.1 2,601,410 + +0.4 2,601,410 + +0.2 2,601,410 + +0.1 2,601,418 + +0.7 2,601,429 - +2.0 2,601,467 - +1.7 2,601,470 + -0.0 2,601,471 - +0.4 2,601,585 + rppH HEPCGN_19255 0.28 -1.9 2,601,613 + rppH HEPCGN_19255 0.34 -0.7 2,601,614 - rppH HEPCGN_19255 0.34 -1.4 2,601,664 - rppH HEPCGN_19255 0.43 -1.6
Or see this region's nucleotide sequence