Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnD and HEPCGN_11270 are separated by 64 nucleotides HEPCGN_11270 and phnF are separated by 20 nucleotides phnF and phnG are separated by 0 nucleotides phnG and phnH overlap by 4 nucleotides
HEPCGN_11265: phnD - phosphonate ABC transporter substrate-binding protein, at 993,003 to 994,019
phnD
HEPCGN_11270: HEPCGN_11270 - Phosphonate ABC transporter permease, at 994,084 to 994,863
_11270
HEPCGN_11275: phnF - phosphonate metabolism transcriptional regulator PhnF, at 994,884 to 995,609
phnF
HEPCGN_11280: phnG - phosphonate C-P lyase system protein PhnG, at 995,610 to 996,062
phnG
HEPCGN_11285: phnH - phosphonate C-P lyase system protein PhnH, at 996,059 to 996,643
phnH
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1 at 994.000 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.018 kb on - strand at 994.197 kb on + strand, within HEPCGN_11270 at 994.276 kb on + strand, within HEPCGN_11270 at 994.394 kb on - strand, within HEPCGN_11270 at 994.404 kb on - strand, within HEPCGN_11270 at 994.663 kb on + strand, within HEPCGN_11270 at 994.879 kb on + strand at 994.879 kb on + strand at 994.879 kb on + strand at 994.883 kb on + strand at 994.883 kb on + strand at 994.883 kb on + strand at 994.884 kb on - strand at 994.895 kb on + strand at 994.983 kb on + strand, within phnF at 994.983 kb on + strand, within phnF at 994.984 kb on - strand, within phnF at 995.318 kb on - strand, within phnF at 995.328 kb on + strand, within phnF at 995.329 kb on - strand, within phnF at 995.492 kb on + strand, within phnF at 995.493 kb on - strand, within phnF at 995.850 kb on + strand, within phnG at 995.979 kb on - strand, within phnG at 996.079 kb on + strand at 996.079 kb on + strand at 996.123 kb on + strand, within phnH at 996.261 kb on + strand, within phnH at 996.262 kb on - strand, within phnH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 994,000 + +0.4 994,004 + +0.1 994,004 + -0.3 994,004 + +0.2 994,004 + -1.3 994,004 + +1.0 994,004 + -0.1 994,018 - -1.1 994,197 + HEPCGN_11270 0.14 +1.2 994,276 + HEPCGN_11270 0.25 -0.4 994,394 - HEPCGN_11270 0.40 +0.2 994,404 - HEPCGN_11270 0.41 -0.2 994,663 + HEPCGN_11270 0.74 -0.9 994,879 + +0.8 994,879 + -0.1 994,879 + +0.7 994,883 + +0.1 994,883 + -1.0 994,883 + +0.6 994,884 - -2.5 994,895 + +0.9 994,983 + phnF HEPCGN_11275 0.14 -0.4 994,983 + phnF HEPCGN_11275 0.14 +1.0 994,984 - phnF HEPCGN_11275 0.14 +1.0 995,318 - phnF HEPCGN_11275 0.60 -0.1 995,328 + phnF HEPCGN_11275 0.61 -0.2 995,329 - phnF HEPCGN_11275 0.61 +0.2 995,492 + phnF HEPCGN_11275 0.84 -1.7 995,493 - phnF HEPCGN_11275 0.84 -0.3 995,850 + phnG HEPCGN_11280 0.53 +1.7 995,979 - phnG HEPCGN_11280 0.81 +1.0 996,079 + -1.3 996,079 + -0.7 996,123 + phnH HEPCGN_11285 0.11 +0.0 996,261 + phnH HEPCGN_11285 0.35 -0.4 996,262 - phnH HEPCGN_11285 0.35 +0.4
Or see this region's nucleotide sequence