Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaJ and yaaA are separated by 69 nucleotides yaaA and yaaX are separated by 39 nucleotides yaaX and thrC are separated by 213 nucleotides
HEPCGN_09090: yaaJ - Uncharacterized transporter YaaJ, at 564,778 to 566,208
yaaJ
HEPCGN_09095: yaaA - peroxide stress protein YaaA, at 566,278 to 567,054
yaaA
HEPCGN_09100: yaaX - Uncharacterized protein YaaX, at 567,094 to 567,390
yaaX
HEPCGN_09105: thrC - threonine synthase, at 567,604 to 568,890
thrC
Position (kb) 567
568 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 566.098 kb on + strand at 566.099 kb on - strand at 566.102 kb on + strand at 566.103 kb on - strand at 566.103 kb on - strand at 566.289 kb on + strand at 566.290 kb on - strand at 566.416 kb on - strand, within yaaA at 566.527 kb on - strand, within yaaA at 566.724 kb on + strand, within yaaA at 566.724 kb on + strand, within yaaA at 566.724 kb on + strand, within yaaA at 566.725 kb on - strand, within yaaA at 566.725 kb on - strand, within yaaA at 566.800 kb on + strand, within yaaA at 566.801 kb on - strand, within yaaA at 566.801 kb on - strand, within yaaA at 566.806 kb on - strand, within yaaA at 566.897 kb on - strand, within yaaA at 566.976 kb on + strand, within yaaA at 566.989 kb on + strand at 566.989 kb on + strand at 567.053 kb on - strand at 567.091 kb on + strand at 567.092 kb on - strand at 567.092 kb on - strand at 567.092 kb on - strand at 567.154 kb on - strand, within yaaX at 567.209 kb on + strand, within yaaX at 567.209 kb on + strand, within yaaX at 567.210 kb on - strand, within yaaX at 567.271 kb on - strand, within yaaX at 567.284 kb on - strand, within yaaX at 567.289 kb on + strand, within yaaX at 567.308 kb on - strand, within yaaX at 567.313 kb on - strand, within yaaX at 567.313 kb on - strand, within yaaX at 567.372 kb on + strand at 567.372 kb on + strand at 567.372 kb on + strand at 567.372 kb on + strand at 567.373 kb on - strand at 567.395 kb on + strand at 567.405 kb on - strand at 567.484 kb on - strand at 567.565 kb on + strand at 567.591 kb on + strand at 567.599 kb on - strand at 567.599 kb on - strand at 567.599 kb on - strand at 567.606 kb on - strand at 567.606 kb on - strand at 567.628 kb on - strand at 567.674 kb on - strand at 567.674 kb on - strand at 567.782 kb on + strand, within thrC at 567.819 kb on - strand, within thrC at 567.819 kb on - strand, within thrC at 567.846 kb on + strand, within thrC at 567.847 kb on - strand, within thrC at 567.847 kb on - strand, within thrC at 567.898 kb on + strand, within thrC at 567.899 kb on - strand, within thrC at 567.899 kb on - strand, within thrC at 568.059 kb on + strand, within thrC at 568.215 kb on - strand, within thrC at 568.258 kb on - strand, within thrC at 568.378 kb on - strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 566,098 + +0.2 566,099 - +0.0 566,102 + +0.7 566,103 - -0.1 566,103 - -0.7 566,289 + +0.6 566,290 - +0.2 566,416 - yaaA HEPCGN_09095 0.18 +0.0 566,527 - yaaA HEPCGN_09095 0.32 +1.1 566,724 + yaaA HEPCGN_09095 0.57 +1.5 566,724 + yaaA HEPCGN_09095 0.57 +1.3 566,724 + yaaA HEPCGN_09095 0.57 +0.7 566,725 - yaaA HEPCGN_09095 0.58 -2.5 566,725 - yaaA HEPCGN_09095 0.58 +0.3 566,800 + yaaA HEPCGN_09095 0.67 -1.0 566,801 - yaaA HEPCGN_09095 0.67 +0.5 566,801 - yaaA HEPCGN_09095 0.67 -0.4 566,806 - yaaA HEPCGN_09095 0.68 +0.3 566,897 - yaaA HEPCGN_09095 0.80 -0.4 566,976 + yaaA HEPCGN_09095 0.90 -0.9 566,989 + -0.4 566,989 + -0.3 567,053 - -1.1 567,091 + +1.2 567,092 - -2.0 567,092 - +0.1 567,092 - +0.2 567,154 - yaaX HEPCGN_09100 0.20 -0.9 567,209 + yaaX HEPCGN_09100 0.39 +0.7 567,209 + yaaX HEPCGN_09100 0.39 -0.3 567,210 - yaaX HEPCGN_09100 0.39 +0.5 567,271 - yaaX HEPCGN_09100 0.60 +0.1 567,284 - yaaX HEPCGN_09100 0.64 +0.6 567,289 + yaaX HEPCGN_09100 0.66 +0.6 567,308 - yaaX HEPCGN_09100 0.72 -0.4 567,313 - yaaX HEPCGN_09100 0.74 +0.5 567,313 - yaaX HEPCGN_09100 0.74 +0.7 567,372 + +0.0 567,372 + -1.1 567,372 + -1.3 567,372 + +0.9 567,373 - +0.0 567,395 + +0.1 567,405 - -0.2 567,484 - +1.1 567,565 + +0.6 567,591 + +0.6 567,599 - +0.9 567,599 - -0.7 567,599 - +1.0 567,606 - +0.3 567,606 - -1.6 567,628 - -0.7 567,674 - -4.9 567,674 - -3.9 567,782 + thrC HEPCGN_09105 0.14 -4.8 567,819 - thrC HEPCGN_09105 0.17 -3.5 567,819 - thrC HEPCGN_09105 0.17 -4.6 567,846 + thrC HEPCGN_09105 0.19 -4.6 567,847 - thrC HEPCGN_09105 0.19 -4.9 567,847 - thrC HEPCGN_09105 0.19 -5.9 567,898 + thrC HEPCGN_09105 0.23 -2.9 567,899 - thrC HEPCGN_09105 0.23 -4.1 567,899 - thrC HEPCGN_09105 0.23 -5.3 568,059 + thrC HEPCGN_09105 0.35 -4.6 568,215 - thrC HEPCGN_09105 0.47 -5.3 568,258 - thrC HEPCGN_09105 0.51 -2.8 568,378 - thrC HEPCGN_09105 0.60 -6.9
Or see this region's nucleotide sequence