Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Asp_trna and dnaQ are separated by 132 nucleotides dnaQ and rnhA are separated by 55 nucleotides rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides
HEPCGN_07900: Asp_trna - tRNA-Asp(gtc), at 319,738 to 319,814
_trna
HEPCGN_07905: dnaQ - DNA polymerase III subunit epsilon, at 319,947 to 320,687
dnaQ
HEPCGN_07910: rnhA - ribonuclease HI, at 320,743 to 321,210
rnhA
HEPCGN_07915: yafS - Uncharacterized protein YafS, at 321,207 to 321,929
yafS
HEPCGN_07920: gloB - hydroxyacylglutathione hydrolase, at 321,963 to 322,718
gloB
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 319.948 kb on + strand at 319.949 kb on - strand at 320.678 kb on + strand at 320.690 kb on - strand at 320.725 kb on - strand at 320.725 kb on - strand at 320.748 kb on + strand at 320.748 kb on + strand at 320.749 kb on - strand at 320.819 kb on + strand, within rnhA at 320.820 kb on - strand, within rnhA at 320.820 kb on - strand, within rnhA at 320.847 kb on + strand, within rnhA at 320.959 kb on + strand, within rnhA at 320.960 kb on - strand, within rnhA at 320.991 kb on + strand, within rnhA at 321.222 kb on - strand at 321.326 kb on + strand, within yafS at 321.326 kb on + strand, within yafS at 321.334 kb on - strand, within yafS at 321.417 kb on + strand, within yafS at 321.468 kb on - strand, within yafS at 321.482 kb on + strand, within yafS at 321.482 kb on + strand, within yafS at 321.482 kb on + strand, within yafS at 321.483 kb on - strand, within yafS at 321.484 kb on + strand, within yafS at 321.485 kb on - strand, within yafS at 321.554 kb on + strand, within yafS at 321.635 kb on + strand, within yafS at 321.636 kb on - strand, within yafS at 321.636 kb on - strand, within yafS at 321.957 kb on + strand at 321.957 kb on + strand at 321.957 kb on + strand at 321.958 kb on - strand at 321.963 kb on - strand at 321.971 kb on + strand at 321.972 kb on - strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.108 kb on + strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.208 kb on + strand, within gloB at 322.209 kb on - strand, within gloB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 319,948 + -0.7 319,949 - -2.0 320,678 + -1.9 320,690 - +1.8 320,725 - +0.7 320,725 - +1.1 320,748 + -0.1 320,748 + +0.9 320,749 - -0.5 320,819 + rnhA HEPCGN_07910 0.16 -3.2 320,820 - rnhA HEPCGN_07910 0.16 -1.2 320,820 - rnhA HEPCGN_07910 0.16 +0.8 320,847 + rnhA HEPCGN_07910 0.22 -1.5 320,959 + rnhA HEPCGN_07910 0.46 -0.4 320,960 - rnhA HEPCGN_07910 0.46 -0.8 320,991 + rnhA HEPCGN_07910 0.53 +0.1 321,222 - +0.4 321,326 + yafS HEPCGN_07915 0.16 +0.2 321,326 + yafS HEPCGN_07915 0.16 -0.2 321,334 - yafS HEPCGN_07915 0.18 -1.0 321,417 + yafS HEPCGN_07915 0.29 -2.5 321,468 - yafS HEPCGN_07915 0.36 +1.3 321,482 + yafS HEPCGN_07915 0.38 -0.4 321,482 + yafS HEPCGN_07915 0.38 -1.3 321,482 + yafS HEPCGN_07915 0.38 -1.6 321,483 - yafS HEPCGN_07915 0.38 +0.6 321,484 + yafS HEPCGN_07915 0.38 -0.5 321,485 - yafS HEPCGN_07915 0.38 +1.9 321,554 + yafS HEPCGN_07915 0.48 -0.3 321,635 + yafS HEPCGN_07915 0.59 +0.1 321,636 - yafS HEPCGN_07915 0.59 -0.6 321,636 - yafS HEPCGN_07915 0.59 -0.0 321,957 + +1.1 321,957 + -1.0 321,957 + +1.0 321,958 - +1.9 321,963 - -1.2 321,971 + +0.9 321,972 - +0.7 321,999 + -0.4 321,999 + -0.3 321,999 + -0.3 321,999 + -1.3 322,000 - -1.1 322,000 - +0.3 322,000 - +0.6 322,108 + gloB HEPCGN_07920 0.19 -3.2 322,186 - gloB HEPCGN_07920 0.29 +0.4 322,186 - gloB HEPCGN_07920 0.29 -0.6 322,186 - gloB HEPCGN_07920 0.29 +1.3 322,208 + gloB HEPCGN_07920 0.32 +0.5 322,209 - gloB HEPCGN_07920 0.33 +3.5
Or see this region's nucleotide sequence