Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliL are separated by 4 nucleotides fliL and fliK are separated by 104 nucleotides fliK and fliJ overlap by 4 nucleotides fliJ and fliI are separated by 18 nucleotides
HEPCGN_04040: fliM - flagellar motor switch protein FliM, at 708,262 to 709,266
fliM
HEPCGN_04045: fliL - flagellar basal body-associated protein FliL, at 709,271 to 709,735
fliL
HEPCGN_04050: fliK - flagellar hook length control protein FliK, at 709,840 to 710,967
fliK
HEPCGN_04055: fliJ - flagella biosynthesis chaperone FliJ, at 710,964 to 711,407
fliJ
HEPCGN_04060: fliI - flagellum-specific ATP synthase FliI, at 711,426 to 712,799
fliI
Position (kb) 709
710
711 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 708.954 kb on + strand, within fliM at 708.954 kb on + strand, within fliM at 709.002 kb on - strand, within fliM at 709.095 kb on + strand, within fliM at 709.254 kb on + strand at 709.282 kb on + strand at 709.293 kb on + strand at 709.294 kb on - strand at 709.458 kb on + strand, within fliL at 709.513 kb on + strand, within fliL at 709.515 kb on + strand, within fliL at 709.515 kb on + strand, within fliL at 709.516 kb on - strand, within fliL at 709.516 kb on - strand, within fliL at 709.516 kb on - strand, within fliL at 709.555 kb on + strand, within fliL at 709.567 kb on - strand, within fliL at 709.767 kb on - strand at 709.908 kb on + strand at 710.130 kb on + strand, within fliK at 710.130 kb on + strand, within fliK at 710.452 kb on - strand, within fliK at 710.598 kb on + strand, within fliK at 711.355 kb on - strand, within fliJ at 711.355 kb on - strand, within fliJ at 711.447 kb on - strand at 711.509 kb on - strand at 711.509 kb on - strand at 711.509 kb on - strand at 711.572 kb on - strand, within fliI at 711.855 kb on - strand, within fliI at 711.917 kb on + strand, within fliI
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 708,954 + fliM HEPCGN_04040 0.69 +2.3 708,954 + fliM HEPCGN_04040 0.69 -3.3 709,002 - fliM HEPCGN_04040 0.74 -0.6 709,095 + fliM HEPCGN_04040 0.83 +0.0 709,254 + +0.5 709,282 + -4.0 709,293 + -0.6 709,294 - +1.0 709,458 + fliL HEPCGN_04045 0.40 -0.4 709,513 + fliL HEPCGN_04045 0.52 +0.5 709,515 + fliL HEPCGN_04045 0.52 -0.2 709,515 + fliL HEPCGN_04045 0.52 +0.7 709,516 - fliL HEPCGN_04045 0.53 +2.5 709,516 - fliL HEPCGN_04045 0.53 +2.1 709,516 - fliL HEPCGN_04045 0.53 +0.6 709,555 + fliL HEPCGN_04045 0.61 +0.0 709,567 - fliL HEPCGN_04045 0.64 -1.0 709,767 - +1.1 709,908 + +0.2 710,130 + fliK HEPCGN_04050 0.26 +0.0 710,130 + fliK HEPCGN_04050 0.26 -0.1 710,452 - fliK HEPCGN_04050 0.54 +0.0 710,598 + fliK HEPCGN_04050 0.67 -1.3 711,355 - fliJ HEPCGN_04055 0.88 -1.5 711,355 - fliJ HEPCGN_04055 0.88 -0.4 711,447 - -0.4 711,509 - -3.3 711,509 - -0.1 711,509 - -0.4 711,572 - fliI HEPCGN_04060 0.11 -0.3 711,855 - fliI HEPCGN_04060 0.31 +1.5 711,917 + fliI HEPCGN_04060 0.36 +0.2
Or see this region's nucleotide sequence