Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapA and HEPCGN_25745 are separated by 312 nucleotides HEPCGN_25745 and puuP are separated by 133 nucleotides puuP and puuA are separated by 302 nucleotides
HEPCGN_25740: sapA - peptide ABC transporter substrate-binding protein SapA, at 3,886,709 to 3,888,352
sapA
HEPCGN_25745: HEPCGN_25745 - DUF2543 domain-containing protein, at 3,888,665 to 3,888,910
_25745
HEPCGN_25750: puuP - Putrescine importer PuuP, at 3,889,044 to 3,890,306
puuP
HEPCGN_25755: puuA - glutamate-putrescine ligase, at 3,890,609 to 3,892,027
puuA
Position (kb)
3889
3890
3891 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3888.510 kb on + strand at 3888.510 kb on + strand at 3888.514 kb on + strand at 3888.514 kb on + strand at 3888.515 kb on - strand at 3888.517 kb on - strand at 3888.517 kb on - strand at 3888.638 kb on - strand at 3888.667 kb on - strand at 3888.673 kb on + strand at 3888.722 kb on - strand, within HEPCGN_25745 at 3888.798 kb on - strand, within HEPCGN_25745 at 3888.807 kb on + strand, within HEPCGN_25745 at 3888.941 kb on + strand at 3888.976 kb on - strand at 3888.976 kb on - strand at 3888.976 kb on - strand at 3889.045 kb on + strand at 3889.046 kb on - strand at 3889.078 kb on + strand at 3889.078 kb on + strand at 3889.078 kb on + strand at 3889.079 kb on - strand at 3889.079 kb on - strand at 3889.111 kb on + strand at 3889.120 kb on + strand at 3889.121 kb on - strand at 3889.229 kb on - strand, within puuP at 3889.299 kb on + strand, within puuP at 3889.299 kb on + strand, within puuP at 3889.300 kb on - strand, within puuP at 3889.395 kb on - strand, within puuP at 3889.435 kb on + strand, within puuP at 3889.480 kb on + strand, within puuP at 3889.481 kb on - strand, within puuP at 3889.544 kb on + strand, within puuP at 3889.544 kb on + strand, within puuP at 3889.544 kb on + strand, within puuP at 3889.583 kb on - strand, within puuP at 3889.978 kb on + strand, within puuP at 3890.007 kb on - strand, within puuP at 3890.041 kb on + strand, within puuP at 3890.046 kb on - strand, within puuP at 3890.048 kb on - strand, within puuP at 3890.132 kb on - strand, within puuP at 3890.181 kb on - strand at 3890.206 kb on + strand at 3890.282 kb on + strand at 3890.298 kb on - strand at 3890.307 kb on - strand at 3890.327 kb on - strand at 3890.329 kb on + strand at 3890.330 kb on - strand at 3890.330 kb on - strand at 3890.330 kb on - strand at 3890.330 kb on - strand at 3890.330 kb on - strand at 3890.369 kb on - strand at 3890.486 kb on - strand at 3890.694 kb on + strand at 3890.695 kb on - strand at 3890.786 kb on + strand, within puuA at 3890.809 kb on + strand, within puuA at 3890.956 kb on + strand, within puuA at 3891.009 kb on + strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.010 kb on - strand, within puuA at 3891.013 kb on + strand, within puuA at 3891.013 kb on + strand, within puuA at 3891.013 kb on + strand, within puuA at 3891.013 kb on + strand, within puuA at 3891.013 kb on + strand, within puuA at 3891.014 kb on - strand, within puuA at 3891.014 kb on - strand, within puuA at 3891.181 kb on + strand, within puuA at 3891.210 kb on + strand, within puuA at 3891.242 kb on - strand, within puuA at 3891.266 kb on + strand, within puuA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,888,510 + +0.8 3,888,510 + +1.2 3,888,514 + +0.9 3,888,514 + -0.5 3,888,515 - -0.3 3,888,517 - +1.4 3,888,517 - +1.8 3,888,638 - +0.5 3,888,667 - -0.5 3,888,673 + +0.0 3,888,722 - HEPCGN_25745 0.23 +2.2 3,888,798 - HEPCGN_25745 0.54 +0.1 3,888,807 + HEPCGN_25745 0.58 +0.1 3,888,941 + +0.1 3,888,976 - +0.9 3,888,976 - +0.3 3,888,976 - +0.9 3,889,045 + +0.5 3,889,046 - +0.1 3,889,078 + -0.8 3,889,078 + +0.4 3,889,078 + +0.1 3,889,079 - -0.2 3,889,079 - -2.3 3,889,111 + +1.2 3,889,120 + +0.3 3,889,121 - +0.5 3,889,229 - puuP HEPCGN_25750 0.15 +0.7 3,889,299 + puuP HEPCGN_25750 0.20 +0.7 3,889,299 + puuP HEPCGN_25750 0.20 -0.1 3,889,300 - puuP HEPCGN_25750 0.20 -0.3 3,889,395 - puuP HEPCGN_25750 0.28 +0.7 3,889,435 + puuP HEPCGN_25750 0.31 +0.4 3,889,480 + puuP HEPCGN_25750 0.35 +0.8 3,889,481 - puuP HEPCGN_25750 0.35 -2.3 3,889,544 + puuP HEPCGN_25750 0.40 +0.7 3,889,544 + puuP HEPCGN_25750 0.40 -0.3 3,889,544 + puuP HEPCGN_25750 0.40 +0.2 3,889,583 - puuP HEPCGN_25750 0.43 +0.0 3,889,978 + puuP HEPCGN_25750 0.74 -0.1 3,890,007 - puuP HEPCGN_25750 0.76 +0.1 3,890,041 + puuP HEPCGN_25750 0.79 +0.2 3,890,046 - puuP HEPCGN_25750 0.79 +0.7 3,890,048 - puuP HEPCGN_25750 0.79 -1.1 3,890,132 - puuP HEPCGN_25750 0.86 -0.0 3,890,181 - +1.6 3,890,206 + +0.5 3,890,282 + +0.4 3,890,298 - +0.7 3,890,307 - +0.1 3,890,327 - +1.1 3,890,329 + +0.7 3,890,330 - +0.2 3,890,330 - +0.4 3,890,330 - -1.1 3,890,330 - -0.1 3,890,330 - +0.1 3,890,369 - +0.7 3,890,486 - -0.4 3,890,694 + -1.7 3,890,695 - +1.1 3,890,786 + puuA HEPCGN_25755 0.12 -0.3 3,890,809 + puuA HEPCGN_25755 0.14 -0.2 3,890,956 + puuA HEPCGN_25755 0.24 +0.2 3,891,009 + puuA HEPCGN_25755 0.28 -2.8 3,891,010 - puuA HEPCGN_25755 0.28 +0.4 3,891,010 - puuA HEPCGN_25755 0.28 +0.4 3,891,010 - puuA HEPCGN_25755 0.28 +0.1 3,891,010 - puuA HEPCGN_25755 0.28 +0.9 3,891,010 - puuA HEPCGN_25755 0.28 +1.2 3,891,010 - puuA HEPCGN_25755 0.28 -0.9 3,891,010 - puuA HEPCGN_25755 0.28 +0.1 3,891,013 + puuA HEPCGN_25755 0.28 +0.2 3,891,013 + puuA HEPCGN_25755 0.28 +2.5 3,891,013 + puuA HEPCGN_25755 0.28 +2.0 3,891,013 + puuA HEPCGN_25755 0.28 +0.8 3,891,013 + puuA HEPCGN_25755 0.28 +1.3 3,891,014 - puuA HEPCGN_25755 0.29 +0.2 3,891,014 - puuA HEPCGN_25755 0.29 -0.8 3,891,181 + puuA HEPCGN_25755 0.40 -0.7 3,891,210 + puuA HEPCGN_25755 0.42 +1.1 3,891,242 - puuA HEPCGN_25755 0.45 -0.6 3,891,266 + puuA HEPCGN_25755 0.46 -0.0
Or see this region's nucleotide sequence