Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoA and iraP are separated by 100 nucleotides iraP and ddlA are separated by 462 nucleotides ddlA and yaiZ are separated by 23 nucleotides yaiZ and yaiY are separated by 259 nucleotides yaiY and yaiW are separated by 58 nucleotides
BNILDI_22965: phoA - alkaline phosphatase, at 4,621,766 to 4,623,181
phoA
BNILDI_22970: iraP - anti-adapter protein IraP, at 4,623,282 to 4,623,542
iraP
BNILDI_22975: ddlA - D-alanine--D-alanine ligase, at 4,624,005 to 4,625,099
ddlA
BNILDI_22980: yaiZ - Uncharacterized protein YaiZ, at 4,625,123 to 4,625,335
yaiZ
BNILDI_22985: yaiY - Inner membrane protein YaiY, at 4,625,595 to 4,625,903
yaiY
BNILDI_22990: yaiW - surface-exposed outer membrane lipoprotein YaiW, at 4,625,962 to 4,627,056
yaiW
Position (kb)
4624
4625
4626 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4623.197 kb on + strand at 4623.232 kb on + strand at 4623.373 kb on + strand, within iraP at 4623.373 kb on + strand, within iraP at 4623.387 kb on + strand, within iraP at 4623.425 kb on + strand, within iraP at 4623.426 kb on - strand, within iraP at 4623.426 kb on - strand, within iraP at 4623.548 kb on + strand at 4623.549 kb on - strand at 4623.549 kb on - strand at 4623.582 kb on + strand at 4623.616 kb on - strand at 4623.649 kb on - strand at 4623.711 kb on - strand at 4623.810 kb on + strand at 4623.810 kb on + strand at 4623.892 kb on + strand at 4623.965 kb on + strand at 4623.966 kb on - strand at 4623.966 kb on - strand at 4623.970 kb on + strand at 4623.971 kb on - strand at 4624.001 kb on + strand at 4624.021 kb on + strand at 4624.101 kb on - strand at 4624.181 kb on - strand, within ddlA at 4624.247 kb on + strand, within ddlA at 4624.387 kb on + strand, within ddlA at 4624.387 kb on + strand, within ddlA at 4624.479 kb on - strand, within ddlA at 4624.532 kb on + strand, within ddlA at 4624.556 kb on - strand, within ddlA at 4624.598 kb on - strand, within ddlA at 4624.652 kb on + strand, within ddlA at 4624.782 kb on + strand, within ddlA at 4624.782 kb on + strand, within ddlA at 4624.782 kb on + strand, within ddlA at 4624.783 kb on - strand, within ddlA at 4624.783 kb on - strand, within ddlA at 4624.906 kb on + strand, within ddlA at 4624.906 kb on + strand, within ddlA at 4624.982 kb on + strand, within ddlA at 4624.982 kb on + strand, within ddlA at 4624.982 kb on + strand, within ddlA at 4624.982 kb on + strand, within ddlA at 4624.983 kb on - strand, within ddlA at 4624.983 kb on - strand, within ddlA at 4624.986 kb on + strand, within ddlA at 4624.987 kb on - strand, within ddlA at 4625.020 kb on - strand at 4625.119 kb on - strand at 4625.119 kb on - strand at 4625.186 kb on - strand, within yaiZ at 4625.186 kb on - strand, within yaiZ at 4625.273 kb on + strand, within yaiZ at 4625.275 kb on + strand, within yaiZ at 4625.275 kb on + strand, within yaiZ at 4625.295 kb on + strand, within yaiZ at 4625.298 kb on + strand, within yaiZ at 4625.299 kb on - strand, within yaiZ at 4625.299 kb on - strand, within yaiZ at 4625.370 kb on + strand at 4625.396 kb on - strand at 4625.521 kb on - strand at 4625.729 kb on - strand, within yaiY at 4625.749 kb on - strand, within yaiY at 4625.803 kb on + strand, within yaiY at 4625.803 kb on + strand, within yaiY at 4625.803 kb on + strand, within yaiY at 4625.840 kb on - strand, within yaiY at 4625.973 kb on + strand at 4625.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,623,197 + +0.2 4,623,232 + -0.8 4,623,373 + iraP BNILDI_22970 0.35 +0.3 4,623,373 + iraP BNILDI_22970 0.35 -0.2 4,623,387 + iraP BNILDI_22970 0.40 +0.6 4,623,425 + iraP BNILDI_22970 0.55 +0.4 4,623,426 - iraP BNILDI_22970 0.55 +1.1 4,623,426 - iraP BNILDI_22970 0.55 +0.5 4,623,548 + +0.8 4,623,549 - +0.6 4,623,549 - +1.0 4,623,582 + +0.3 4,623,616 - +0.9 4,623,649 - -1.2 4,623,711 - -0.9 4,623,810 + +0.8 4,623,810 + -1.1 4,623,892 + -0.3 4,623,965 + -0.4 4,623,966 - +0.1 4,623,966 - -1.0 4,623,970 + +0.0 4,623,971 - -2.1 4,624,001 + -0.3 4,624,021 + +0.2 4,624,101 - -0.2 4,624,181 - ddlA BNILDI_22975 0.16 +0.4 4,624,247 + ddlA BNILDI_22975 0.22 +0.4 4,624,387 + ddlA BNILDI_22975 0.35 -0.8 4,624,387 + ddlA BNILDI_22975 0.35 -1.3 4,624,479 - ddlA BNILDI_22975 0.43 +0.1 4,624,532 + ddlA BNILDI_22975 0.48 +0.6 4,624,556 - ddlA BNILDI_22975 0.50 -0.1 4,624,598 - ddlA BNILDI_22975 0.54 +0.0 4,624,652 + ddlA BNILDI_22975 0.59 +0.4 4,624,782 + ddlA BNILDI_22975 0.71 -2.8 4,624,782 + ddlA BNILDI_22975 0.71 -0.9 4,624,782 + ddlA BNILDI_22975 0.71 +0.4 4,624,783 - ddlA BNILDI_22975 0.71 -2.0 4,624,783 - ddlA BNILDI_22975 0.71 -0.0 4,624,906 + ddlA BNILDI_22975 0.82 -0.0 4,624,906 + ddlA BNILDI_22975 0.82 +0.3 4,624,982 + ddlA BNILDI_22975 0.89 -0.0 4,624,982 + ddlA BNILDI_22975 0.89 -1.1 4,624,982 + ddlA BNILDI_22975 0.89 +0.1 4,624,982 + ddlA BNILDI_22975 0.89 -0.0 4,624,983 - ddlA BNILDI_22975 0.89 -0.8 4,624,983 - ddlA BNILDI_22975 0.89 +0.2 4,624,986 + ddlA BNILDI_22975 0.90 -0.1 4,624,987 - ddlA BNILDI_22975 0.90 +0.2 4,625,020 - +0.8 4,625,119 - +1.3 4,625,119 - +0.3 4,625,186 - yaiZ BNILDI_22980 0.30 -0.0 4,625,186 - yaiZ BNILDI_22980 0.30 +0.6 4,625,273 + yaiZ BNILDI_22980 0.70 +0.8 4,625,275 + yaiZ BNILDI_22980 0.71 +0.9 4,625,275 + yaiZ BNILDI_22980 0.71 +0.1 4,625,295 + yaiZ BNILDI_22980 0.81 +0.3 4,625,298 + yaiZ BNILDI_22980 0.82 -0.4 4,625,299 - yaiZ BNILDI_22980 0.83 +0.3 4,625,299 - yaiZ BNILDI_22980 0.83 -0.5 4,625,370 + +0.5 4,625,396 - -0.1 4,625,521 - +0.2 4,625,729 - yaiY BNILDI_22985 0.43 -1.1 4,625,749 - yaiY BNILDI_22985 0.50 -1.5 4,625,803 + yaiY BNILDI_22985 0.67 +0.5 4,625,803 + yaiY BNILDI_22985 0.67 -0.3 4,625,803 + yaiY BNILDI_22985 0.67 +0.3 4,625,840 - yaiY BNILDI_22985 0.79 -1.7 4,625,973 + +0.2 4,625,973 + -0.7
Or see this region's nucleotide sequence