Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dxs and yajO are separated by 179 nucleotides yajO and pgpA are separated by 53 nucleotides pgpA and thiL overlap by 23 nucleotides
BNILDI_22785: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 4,584,802 to 4,586,664
dxs
BNILDI_22790: yajO - 1-deoxyxylulose-5-phosphate synthase YajO, at 4,586,844 to 4,587,818
yajO
BNILDI_22795: pgpA - phosphatidylglycerophosphatase A, at 4,587,872 to 4,588,390
pgpA
BNILDI_22800: thiL - thiamine-phosphate kinase, at 4,588,368 to 4,589,345
thiL
Position (kb) 4586
4587
4588 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4586.710 kb on + strand at 4586.715 kb on - strand at 4586.762 kb on - strand at 4586.762 kb on - strand at 4586.762 kb on - strand at 4586.765 kb on + strand at 4586.765 kb on + strand at 4586.765 kb on + strand at 4586.765 kb on + strand at 4586.766 kb on - strand at 4586.766 kb on - strand at 4586.766 kb on - strand at 4586.766 kb on - strand at 4586.770 kb on - strand at 4586.818 kb on - strand at 4586.850 kb on + strand at 4587.020 kb on + strand, within yajO at 4587.021 kb on - strand, within yajO at 4587.021 kb on - strand, within yajO at 4587.024 kb on + strand, within yajO at 4587.025 kb on - strand, within yajO at 4587.280 kb on + strand, within yajO at 4587.303 kb on - strand, within yajO at 4587.381 kb on - strand, within yajO at 4587.386 kb on + strand, within yajO at 4587.399 kb on + strand, within yajO at 4587.399 kb on + strand, within yajO at 4587.668 kb on + strand, within yajO at 4587.727 kb on + strand at 4587.787 kb on - strand at 4587.816 kb on + strand at 4587.873 kb on + strand at 4587.874 kb on - strand at 4587.912 kb on + strand at 4587.913 kb on - strand at 4587.920 kb on - strand at 4587.977 kb on + strand, within pgpA at 4587.978 kb on - strand, within pgpA at 4588.018 kb on + strand, within pgpA at 4588.019 kb on - strand, within pgpA at 4588.174 kb on - strand, within pgpA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,586,710 + -0.1 4,586,715 - +0.3 4,586,762 - -0.7 4,586,762 - +0.3 4,586,762 - -2.5 4,586,765 + -0.5 4,586,765 + +0.0 4,586,765 + -0.0 4,586,765 + -0.1 4,586,766 - +0.7 4,586,766 - -0.5 4,586,766 - +0.7 4,586,766 - +0.5 4,586,770 - +0.6 4,586,818 - +0.5 4,586,850 + +1.1 4,587,020 + yajO BNILDI_22790 0.18 +0.2 4,587,021 - yajO BNILDI_22790 0.18 +0.3 4,587,021 - yajO BNILDI_22790 0.18 -0.6 4,587,024 + yajO BNILDI_22790 0.18 +0.7 4,587,025 - yajO BNILDI_22790 0.19 +0.0 4,587,280 + yajO BNILDI_22790 0.45 -0.4 4,587,303 - yajO BNILDI_22790 0.47 +0.0 4,587,381 - yajO BNILDI_22790 0.55 -0.2 4,587,386 + yajO BNILDI_22790 0.56 -1.2 4,587,399 + yajO BNILDI_22790 0.57 +0.0 4,587,399 + yajO BNILDI_22790 0.57 -2.9 4,587,668 + yajO BNILDI_22790 0.85 -1.0 4,587,727 + +0.5 4,587,787 - +0.6 4,587,816 + -0.4 4,587,873 + -2.3 4,587,874 - +0.9 4,587,912 + -0.9 4,587,913 - +0.6 4,587,920 - +0.1 4,587,977 + pgpA BNILDI_22795 0.20 +0.3 4,587,978 - pgpA BNILDI_22795 0.20 +0.5 4,588,018 + pgpA BNILDI_22795 0.28 +0.4 4,588,019 - pgpA BNILDI_22795 0.28 -0.2 4,588,174 - pgpA BNILDI_22795 0.58 -0.2
Or see this region's nucleotide sequence