Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panE and yajL overlap by 38 nucleotides yajL and thiI are separated by 53 nucleotides thiI and xseB are separated by 205 nucleotides xseB and ispA overlap by 1 nucleotides
BNILDI_22760: panE - 2-dehydropantoate 2-reductase, at 4,580,464 to 4,581,375
panE
BNILDI_22765: yajL - protein deglycase YajL, at 4,581,338 to 4,581,928
yajL
BNILDI_22770: thiI - tRNA sulfurtransferase, at 4,581,982 to 4,583,430
thiI
BNILDI_22775: xseB - exodeoxyribonuclease VII small subunit, at 4,583,636 to 4,583,878
xseB
BNILDI_22780: ispA - (2E,6E)-farnesyl diphosphate synthase, at 4,583,878 to 4,584,777
ispA
Position (kb)
4581
4582
4583
4584 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4581.053 kb on + strand, within panE at 4581.053 kb on + strand, within panE at 4581.054 kb on - strand, within panE at 4581.083 kb on + strand, within panE at 4581.239 kb on - strand, within panE at 4581.239 kb on - strand, within panE at 4581.258 kb on - strand, within panE at 4581.318 kb on - strand at 4581.400 kb on + strand, within yajL at 4581.473 kb on - strand, within yajL at 4581.592 kb on + strand, within yajL at 4581.593 kb on - strand, within yajL at 4581.593 kb on - strand, within yajL at 4581.704 kb on - strand, within yajL at 4581.707 kb on - strand, within yajL at 4581.707 kb on - strand, within yajL at 4581.911 kb on + strand at 4581.914 kb on - strand at 4581.914 kb on - strand at 4581.918 kb on - strand at 4581.918 kb on - strand at 4581.970 kb on + strand at 4581.970 kb on + strand at 4581.994 kb on + strand at 4581.994 kb on + strand at 4581.995 kb on - strand at 4581.995 kb on - strand at 4582.008 kb on + strand at 4582.185 kb on + strand, within thiI at 4582.291 kb on - strand, within thiI at 4582.403 kb on + strand, within thiI at 4582.632 kb on + strand, within thiI at 4582.632 kb on + strand, within thiI at 4582.632 kb on + strand, within thiI at 4582.632 kb on + strand, within thiI at 4582.632 kb on + strand, within thiI at 4582.633 kb on - strand, within thiI at 4582.705 kb on - strand, within thiI at 4582.815 kb on - strand, within thiI at 4582.845 kb on + strand, within thiI at 4582.845 kb on + strand, within thiI at 4582.845 kb on + strand, within thiI at 4582.846 kb on - strand, within thiI at 4582.846 kb on - strand, within thiI at 4583.026 kb on - strand, within thiI at 4583.036 kb on - strand, within thiI at 4583.051 kb on + strand, within thiI at 4583.321 kb on + strand at 4583.322 kb on - strand at 4583.330 kb on - strand at 4583.421 kb on + strand at 4583.468 kb on + strand at 4583.468 kb on + strand at 4583.468 kb on + strand at 4583.468 kb on + strand at 4583.480 kb on + strand at 4583.522 kb on + strand at 4583.523 kb on - strand at 4583.528 kb on + strand at 4583.556 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,581,053 + panE BNILDI_22760 0.65 +0.1 4,581,053 + panE BNILDI_22760 0.65 -0.2 4,581,054 - panE BNILDI_22760 0.65 -2.2 4,581,083 + panE BNILDI_22760 0.68 -2.6 4,581,239 - panE BNILDI_22760 0.85 -0.4 4,581,239 - panE BNILDI_22760 0.85 -0.7 4,581,258 - panE BNILDI_22760 0.87 +0.1 4,581,318 - +0.2 4,581,400 + yajL BNILDI_22765 0.10 -0.0 4,581,473 - yajL BNILDI_22765 0.23 +0.7 4,581,592 + yajL BNILDI_22765 0.43 -0.6 4,581,593 - yajL BNILDI_22765 0.43 +0.3 4,581,593 - yajL BNILDI_22765 0.43 -0.2 4,581,704 - yajL BNILDI_22765 0.62 +0.8 4,581,707 - yajL BNILDI_22765 0.62 +0.9 4,581,707 - yajL BNILDI_22765 0.62 +0.7 4,581,911 + -0.1 4,581,914 - +1.2 4,581,914 - +0.1 4,581,918 - -3.1 4,581,918 - -0.1 4,581,970 + -0.3 4,581,970 + +0.0 4,581,994 + -1.6 4,581,994 + -0.2 4,581,995 - +0.3 4,581,995 - -0.1 4,582,008 + -0.7 4,582,185 + thiI BNILDI_22770 0.14 -1.4 4,582,291 - thiI BNILDI_22770 0.21 -1.0 4,582,403 + thiI BNILDI_22770 0.29 -0.1 4,582,632 + thiI BNILDI_22770 0.45 -0.2 4,582,632 + thiI BNILDI_22770 0.45 -1.5 4,582,632 + thiI BNILDI_22770 0.45 -0.9 4,582,632 + thiI BNILDI_22770 0.45 -2.9 4,582,632 + thiI BNILDI_22770 0.45 -2.0 4,582,633 - thiI BNILDI_22770 0.45 -0.2 4,582,705 - thiI BNILDI_22770 0.50 +0.6 4,582,815 - thiI BNILDI_22770 0.57 -0.7 4,582,845 + thiI BNILDI_22770 0.60 -1.1 4,582,845 + thiI BNILDI_22770 0.60 -2.8 4,582,845 + thiI BNILDI_22770 0.60 +0.5 4,582,846 - thiI BNILDI_22770 0.60 -2.2 4,582,846 - thiI BNILDI_22770 0.60 -0.7 4,583,026 - thiI BNILDI_22770 0.72 +0.2 4,583,036 - thiI BNILDI_22770 0.73 -1.7 4,583,051 + thiI BNILDI_22770 0.74 -0.9 4,583,321 + -0.7 4,583,322 - -0.9 4,583,330 - -1.2 4,583,421 + -1.7 4,583,468 + +0.1 4,583,468 + +3.2 4,583,468 + -2.3 4,583,468 + -0.4 4,583,480 + -2.1 4,583,522 + -0.4 4,583,523 - +0.5 4,583,528 + +0.0 4,583,556 + +0.4
Or see this region's nucleotide sequence