Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagD and asnB are separated by 256 nucleotides asnB and Met_trna are separated by 380 nucleotides Met_trna and Leu_trna are separated by 8 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides
BNILDI_21560: nagD - ribonucleotide monophosphatase NagD, at 4,338,902 to 4,339,654
nagD
BNILDI_21565: asnB - asparagine synthase B, at 4,339,911 to 4,341,575
asnB
BNILDI_21570: Met_trna - tRNA-Met(cat), at 4,341,956 to 4,342,032
_trna
BNILDI_21575: Leu_trna - tRNA-Leu(tag), at 4,342,041 to 4,342,125
_trna
BNILDI_21580: Gln_trna - tRNA-Gln(ttg), at 4,342,149 to 4,342,223
_trna
BNILDI_21585: Gln_trna - tRNA-Gln(ttg), at 4,342,258 to 4,342,332
_trna
BNILDI_21590: Met_trna - tRNA-Met(cat), at 4,342,348 to 4,342,424
_trna
BNILDI_21595: Gln_trna - tRNA-Gln(ctg), at 4,342,473 to 4,342,547
_trna
Position (kb) 4339
4340
4341
4342 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4339.031 kb on + strand, within nagD at 4339.106 kb on + strand, within nagD at 4339.163 kb on + strand, within nagD at 4339.163 kb on + strand, within nagD at 4339.163 kb on + strand, within nagD at 4339.164 kb on - strand, within nagD at 4339.164 kb on - strand, within nagD at 4339.265 kb on + strand, within nagD at 4339.265 kb on + strand, within nagD at 4339.265 kb on + strand, within nagD at 4339.266 kb on - strand, within nagD at 4339.266 kb on - strand, within nagD at 4339.296 kb on - strand, within nagD at 4339.418 kb on + strand, within nagD at 4339.418 kb on + strand, within nagD at 4339.419 kb on - strand, within nagD at 4339.419 kb on - strand, within nagD at 4339.597 kb on + strand at 4339.597 kb on + strand at 4339.648 kb on + strand at 4339.656 kb on + strand at 4339.719 kb on + strand at 4339.862 kb on - strand at 4339.889 kb on + strand at 4339.889 kb on + strand at 4339.889 kb on + strand at 4339.910 kb on + strand at 4339.910 kb on + strand at 4339.910 kb on + strand at 4339.910 kb on + strand at 4339.911 kb on - strand at 4340.025 kb on + strand at 4340.025 kb on + strand at 4340.100 kb on + strand, within asnB at 4340.100 kb on + strand, within asnB at 4340.311 kb on - strand, within asnB at 4340.349 kb on + strand, within asnB at 4340.349 kb on + strand, within asnB at 4340.349 kb on + strand, within asnB at 4340.350 kb on - strand, within asnB at 4340.350 kb on - strand, within asnB at 4340.350 kb on - strand, within asnB at 4340.350 kb on - strand, within asnB at 4340.352 kb on - strand, within asnB at 4340.379 kb on + strand, within asnB at 4340.380 kb on - strand, within asnB at 4340.448 kb on + strand, within asnB at 4340.449 kb on - strand, within asnB at 4340.449 kb on - strand, within asnB at 4340.481 kb on + strand, within asnB at 4340.481 kb on + strand, within asnB at 4340.482 kb on - strand, within asnB at 4340.482 kb on - strand, within asnB at 4340.482 kb on - strand, within asnB at 4340.713 kb on + strand, within asnB at 4340.714 kb on - strand, within asnB at 4340.815 kb on + strand, within asnB at 4340.815 kb on + strand, within asnB at 4340.850 kb on + strand, within asnB at 4340.851 kb on - strand, within asnB at 4340.865 kb on + strand, within asnB at 4340.901 kb on + strand, within asnB at 4340.902 kb on - strand, within asnB at 4341.039 kb on - strand, within asnB at 4341.056 kb on + strand, within asnB at 4341.056 kb on + strand, within asnB at 4341.057 kb on - strand, within asnB at 4341.057 kb on - strand, within asnB at 4341.057 kb on - strand, within asnB at 4341.057 kb on - strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.060 kb on + strand, within asnB at 4341.061 kb on - strand, within asnB at 4341.061 kb on - strand, within asnB at 4341.229 kb on - strand, within asnB at 4341.396 kb on + strand, within asnB at 4341.397 kb on - strand, within asnB at 4341.567 kb on + strand at 4341.570 kb on - strand at 4341.574 kb on - strand at 4341.649 kb on + strand at 4341.733 kb on + strand at 4341.809 kb on + strand at 4341.810 kb on - strand at 4341.821 kb on - strand at 4341.821 kb on - strand at 4341.845 kb on - strand at 4342.356 kb on - strand, within Met_trna at 4342.443 kb on + strand at 4342.564 kb on + strand at 4342.564 kb on + strand at 4342.564 kb on + strand at 4342.565 kb on - strand at 4342.571 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,339,031 + nagD BNILDI_21560 0.17 -0.7 4,339,106 + nagD BNILDI_21560 0.27 -0.0 4,339,163 + nagD BNILDI_21560 0.35 -0.7 4,339,163 + nagD BNILDI_21560 0.35 -0.2 4,339,163 + nagD BNILDI_21560 0.35 +0.7 4,339,164 - nagD BNILDI_21560 0.35 -0.2 4,339,164 - nagD BNILDI_21560 0.35 +0.2 4,339,265 + nagD BNILDI_21560 0.48 +1.0 4,339,265 + nagD BNILDI_21560 0.48 +1.1 4,339,265 + nagD BNILDI_21560 0.48 +0.1 4,339,266 - nagD BNILDI_21560 0.48 -0.1 4,339,266 - nagD BNILDI_21560 0.48 +0.8 4,339,296 - nagD BNILDI_21560 0.52 +0.0 4,339,418 + nagD BNILDI_21560 0.69 +0.0 4,339,418 + nagD BNILDI_21560 0.69 -0.9 4,339,419 - nagD BNILDI_21560 0.69 -0.3 4,339,419 - nagD BNILDI_21560 0.69 +0.9 4,339,597 + -1.2 4,339,597 + -1.8 4,339,648 + +0.1 4,339,656 + +0.2 4,339,719 + +1.1 4,339,862 - -0.1 4,339,889 + -0.2 4,339,889 + -0.7 4,339,889 + +0.0 4,339,910 + -1.6 4,339,910 + -0.5 4,339,910 + +0.1 4,339,910 + +0.4 4,339,911 - +0.5 4,340,025 + +0.9 4,340,025 + -0.8 4,340,100 + asnB BNILDI_21565 0.11 -0.1 4,340,100 + asnB BNILDI_21565 0.11 +1.7 4,340,311 - asnB BNILDI_21565 0.24 -0.5 4,340,349 + asnB BNILDI_21565 0.26 +0.1 4,340,349 + asnB BNILDI_21565 0.26 -1.0 4,340,349 + asnB BNILDI_21565 0.26 -0.8 4,340,350 - asnB BNILDI_21565 0.26 +0.0 4,340,350 - asnB BNILDI_21565 0.26 -4.0 4,340,350 - asnB BNILDI_21565 0.26 +1.1 4,340,350 - asnB BNILDI_21565 0.26 +0.1 4,340,352 - asnB BNILDI_21565 0.26 +1.0 4,340,379 + asnB BNILDI_21565 0.28 -0.3 4,340,380 - asnB BNILDI_21565 0.28 +0.0 4,340,448 + asnB BNILDI_21565 0.32 +0.5 4,340,449 - asnB BNILDI_21565 0.32 -0.6 4,340,449 - asnB BNILDI_21565 0.32 +0.0 4,340,481 + asnB BNILDI_21565 0.34 -0.4 4,340,481 + asnB BNILDI_21565 0.34 -0.1 4,340,482 - asnB BNILDI_21565 0.34 -0.1 4,340,482 - asnB BNILDI_21565 0.34 -0.9 4,340,482 - asnB BNILDI_21565 0.34 -0.7 4,340,713 + asnB BNILDI_21565 0.48 +2.4 4,340,714 - asnB BNILDI_21565 0.48 -0.3 4,340,815 + asnB BNILDI_21565 0.54 -1.6 4,340,815 + asnB BNILDI_21565 0.54 +1.2 4,340,850 + asnB BNILDI_21565 0.56 -0.0 4,340,851 - asnB BNILDI_21565 0.56 -0.4 4,340,865 + asnB BNILDI_21565 0.57 -1.3 4,340,901 + asnB BNILDI_21565 0.59 -0.3 4,340,902 - asnB BNILDI_21565 0.60 -0.9 4,341,039 - asnB BNILDI_21565 0.68 -0.8 4,341,056 + asnB BNILDI_21565 0.69 +0.3 4,341,056 + asnB BNILDI_21565 0.69 -3.5 4,341,057 - asnB BNILDI_21565 0.69 -0.2 4,341,057 - asnB BNILDI_21565 0.69 -0.4 4,341,057 - asnB BNILDI_21565 0.69 +0.5 4,341,057 - asnB BNILDI_21565 0.69 -0.7 4,341,060 + asnB BNILDI_21565 0.69 -2.3 4,341,060 + asnB BNILDI_21565 0.69 -0.8 4,341,060 + asnB BNILDI_21565 0.69 +0.4 4,341,060 + asnB BNILDI_21565 0.69 -0.4 4,341,060 + asnB BNILDI_21565 0.69 +0.6 4,341,060 + asnB BNILDI_21565 0.69 +0.5 4,341,061 - asnB BNILDI_21565 0.69 +0.3 4,341,061 - asnB BNILDI_21565 0.69 +0.9 4,341,229 - asnB BNILDI_21565 0.79 +0.4 4,341,396 + asnB BNILDI_21565 0.89 -1.3 4,341,397 - asnB BNILDI_21565 0.89 +0.1 4,341,567 + -0.4 4,341,570 - +0.2 4,341,574 - -0.3 4,341,649 + -0.2 4,341,733 + -0.4 4,341,809 + +1.2 4,341,810 - -0.2 4,341,821 - -0.2 4,341,821 - -0.8 4,341,845 - -0.5 4,342,356 - Met_trna BNILDI_21590 0.10 -1.4 4,342,443 + -0.4 4,342,564 + -0.0 4,342,564 + +0.6 4,342,564 + +0.1 4,342,565 - -0.3 4,342,571 + -1.2
Or see this region's nucleotide sequence