Strain Fitness in Escherichia coli ECRC62 around BNILDI_20815

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmntR and mntS are separated by 185 nucleotidesmntS and opgE are separated by 271 nucleotidesopgE and BNILDI_20820 are separated by 48 nucleotidesBNILDI_20820 and yliM are separated by 52 nucleotidesyliM and rhtA are separated by 234 nucleotides BNILDI_20805: mntR - manganese-binding transcriptional regulator MntR, at 4,190,833 to 4,191,300 mntR BNILDI_20810: mntS - manganase accumulation protein MntS, at 4,191,486 to 4,191,614 mntS BNILDI_20815: opgE - phosphoethanolamine transferase, at 4,191,886 to 4,193,469 opgE BNILDI_20820: BNILDI_20820 - Outer membrane protein X, at 4,193,518 to 4,194,033 _20820 BNILDI_20825: yliM - Protein YliM, at 4,194,086 to 4,194,151 yliM BNILDI_20830: rhtA - threonine/homoserine exporter RhtA, at 4,194,386 to 4,195,273 rhtA Position (kb) 4191 4192 4193 4194Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4190.969 kb on - strand, within mntRat 4191.179 kb on + strand, within mntRat 4191.179 kb on + strand, within mntRat 4191.190 kb on - strand, within mntRat 4191.327 kb on - strandat 4191.327 kb on - strandat 4191.327 kb on - strandat 4191.327 kb on - strandat 4191.331 kb on - strandat 4191.331 kb on - strandat 4191.331 kb on - strandat 4191.400 kb on + strandat 4191.409 kb on - strandat 4191.425 kb on + strandat 4191.426 kb on - strandat 4191.435 kb on + strandat 4191.435 kb on + strandat 4191.506 kb on + strand, within mntSat 4191.506 kb on + strand, within mntSat 4191.507 kb on - strand, within mntSat 4191.507 kb on - strand, within mntSat 4191.533 kb on + strand, within mntSat 4191.533 kb on + strand, within mntSat 4191.558 kb on - strand, within mntSat 4191.558 kb on - strand, within mntSat 4191.558 kb on - strand, within mntSat 4191.558 kb on - strand, within mntSat 4191.558 kb on - strand, within mntSat 4191.778 kb on + strandat 4191.780 kb on + strandat 4191.854 kb on + strandat 4191.873 kb on - strandat 4191.968 kb on + strandat 4191.971 kb on + strandat 4191.972 kb on - strandat 4192.149 kb on + strand, within opgEat 4192.217 kb on - strand, within opgEat 4192.222 kb on + strand, within opgEat 4192.223 kb on - strand, within opgEat 4192.235 kb on + strand, within opgEat 4192.325 kb on + strand, within opgEat 4192.383 kb on - strand, within opgEat 4192.386 kb on + strand, within opgEat 4192.386 kb on + strand, within opgEat 4192.531 kb on + strand, within opgEat 4192.540 kb on + strand, within opgEat 4192.540 kb on + strand, within opgEat 4192.541 kb on - strand, within opgEat 4192.640 kb on + strand, within opgEat 4192.651 kb on + strand, within opgEat 4192.652 kb on - strand, within opgEat 4192.652 kb on - strand, within opgEat 4192.808 kb on - strand, within opgEat 4192.852 kb on + strand, within opgEat 4192.852 kb on + strand, within opgEat 4192.852 kb on + strand, within opgEat 4192.853 kb on - strand, within opgEat 4192.968 kb on - strand, within opgEat 4193.018 kb on - strand, within opgEat 4193.018 kb on - strand, within opgEat 4193.084 kb on + strand, within opgEat 4193.099 kb on - strand, within opgEat 4193.191 kb on + strand, within opgEat 4193.192 kb on - strand, within opgEat 4193.208 kb on + strand, within opgEat 4193.208 kb on + strand, within opgEat 4193.209 kb on - strand, within opgEat 4193.209 kb on - strand, within opgEat 4193.370 kb on - strandat 4193.396 kb on - strandat 4193.396 kb on - strandat 4193.396 kb on - strandat 4193.415 kb on - strandat 4193.429 kb on - strandat 4193.520 kb on - strandat 4193.520 kb on - strandat 4193.554 kb on + strandat 4193.555 kb on - strandat 4193.555 kb on - strandat 4193.555 kb on - strandat 4193.565 kb on + strandat 4193.586 kb on - strand, within BNILDI_20820at 4193.727 kb on - strand, within BNILDI_20820at 4193.795 kb on + strand, within BNILDI_20820at 4193.796 kb on - strand, within BNILDI_20820at 4193.814 kb on + strand, within BNILDI_20820at 4193.831 kb on + strand, within BNILDI_20820at 4193.832 kb on - strand, within BNILDI_20820at 4193.969 kb on - strand, within BNILDI_20820at 4194.058 kb on - strandat 4194.076 kb on - strandat 4194.237 kb on - strandat 4194.254 kb on + strandat 4194.333 kb on - strandat 4194.333 kb on - strandat 4194.355 kb on + strandat 4194.359 kb on + strandat 4194.365 kb on + strandat 4194.365 kb on + strandat 4194.403 kb on + strandat 4194.433 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,190,969 - mntR BNILDI_20805 0.29 -0.7
4,191,179 + mntR BNILDI_20805 0.74 +0.5
4,191,179 + mntR BNILDI_20805 0.74 +0.3
4,191,190 - mntR BNILDI_20805 0.76 -1.5
4,191,327 - +0.2
4,191,327 - +0.1
4,191,327 - +0.4
4,191,327 - +1.2
4,191,331 - -0.4
4,191,331 - -0.9
4,191,331 - -0.9
4,191,400 + +0.1
4,191,409 - +0.1
4,191,425 + +0.6
4,191,426 - -0.5
4,191,435 + -0.2
4,191,435 + +0.5
4,191,506 + mntS BNILDI_20810 0.16 +0.0
4,191,506 + mntS BNILDI_20810 0.16 -0.2
4,191,507 - mntS BNILDI_20810 0.16 -0.2
4,191,507 - mntS BNILDI_20810 0.16 +0.4
4,191,533 + mntS BNILDI_20810 0.36 -0.6
4,191,533 + mntS BNILDI_20810 0.36 -0.1
4,191,558 - mntS BNILDI_20810 0.56 +0.6
4,191,558 - mntS BNILDI_20810 0.56 +0.2
4,191,558 - mntS BNILDI_20810 0.56 +0.1
4,191,558 - mntS BNILDI_20810 0.56 +2.1
4,191,558 - mntS BNILDI_20810 0.56 -2.0
4,191,778 + +0.9
4,191,780 + -0.5
4,191,854 + -1.7
4,191,873 - -0.9
4,191,968 + -0.2
4,191,971 + -0.6
4,191,972 - -0.1
4,192,149 + opgE BNILDI_20815 0.17 -2.7
4,192,217 - opgE BNILDI_20815 0.21 +1.3
4,192,222 + opgE BNILDI_20815 0.21 -0.8
4,192,223 - opgE BNILDI_20815 0.21 +0.0
4,192,235 + opgE BNILDI_20815 0.22 -0.5
4,192,325 + opgE BNILDI_20815 0.28 +0.6
4,192,383 - opgE BNILDI_20815 0.31 -1.4
4,192,386 + opgE BNILDI_20815 0.32 -1.1
4,192,386 + opgE BNILDI_20815 0.32 -0.8
4,192,531 + opgE BNILDI_20815 0.41 -1.5
4,192,540 + opgE BNILDI_20815 0.41 +0.2
4,192,540 + opgE BNILDI_20815 0.41 +0.0
4,192,541 - opgE BNILDI_20815 0.41 +1.2
4,192,640 + opgE BNILDI_20815 0.48 -1.0
4,192,651 + opgE BNILDI_20815 0.48 -0.1
4,192,652 - opgE BNILDI_20815 0.48 -0.1
4,192,652 - opgE BNILDI_20815 0.48 -2.2
4,192,808 - opgE BNILDI_20815 0.58 -1.2
4,192,852 + opgE BNILDI_20815 0.61 -0.2
4,192,852 + opgE BNILDI_20815 0.61 +1.7
4,192,852 + opgE BNILDI_20815 0.61 -0.2
4,192,853 - opgE BNILDI_20815 0.61 -0.3
4,192,968 - opgE BNILDI_20815 0.68 -0.5
4,193,018 - opgE BNILDI_20815 0.71 +0.4
4,193,018 - opgE BNILDI_20815 0.71 -3.7
4,193,084 + opgE BNILDI_20815 0.76 -0.3
4,193,099 - opgE BNILDI_20815 0.77 -0.2
4,193,191 + opgE BNILDI_20815 0.82 +2.1
4,193,192 - opgE BNILDI_20815 0.82 +0.6
4,193,208 + opgE BNILDI_20815 0.83 -1.8
4,193,208 + opgE BNILDI_20815 0.83 -0.1
4,193,209 - opgE BNILDI_20815 0.84 +0.7
4,193,209 - opgE BNILDI_20815 0.84 -3.4
4,193,370 - -2.9
4,193,396 - -0.8
4,193,396 - +0.3
4,193,396 - +0.3
4,193,415 - +0.4
4,193,429 - +0.1
4,193,520 - +1.0
4,193,520 - +0.1
4,193,554 + -0.5
4,193,555 - +0.4
4,193,555 - -1.9
4,193,555 - +0.3
4,193,565 + +0.2
4,193,586 - BNILDI_20820 0.13 +0.1
4,193,727 - BNILDI_20820 0.41 +0.5
4,193,795 + BNILDI_20820 0.54 -0.0
4,193,796 - BNILDI_20820 0.54 -0.2
4,193,814 + BNILDI_20820 0.57 -0.2
4,193,831 + BNILDI_20820 0.61 +0.4
4,193,832 - BNILDI_20820 0.61 -0.0
4,193,969 - BNILDI_20820 0.87 -1.0
4,194,058 - +0.1
4,194,076 - -2.1
4,194,237 - +0.6
4,194,254 + +0.1
4,194,333 - +0.2
4,194,333 - -1.0
4,194,355 + -2.4
4,194,359 + +0.2
4,194,365 + +0.7
4,194,365 + -0.8
4,194,403 + -0.8
4,194,433 - -0.2

Or see this region's nucleotide sequence