Strain Fitness in Escherichia coli ECRC62 around BNILDI_19205

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotideslpoB and BNILDI_19210 are separated by 13 nucleotidesBNILDI_19210 and hinT are separated by 2 nucleotides BNILDI_19195: nagZ - beta-N-acetylhexosaminidase, at 3,860,370 to 3,861,395 nagZ BNILDI_19200: thiK - thiamine kinase, at 3,861,406 to 3,862,230 thiK BNILDI_19205: lpoB - penicillin-binding protein activator LpoB, at 3,862,211 to 3,862,852 lpoB BNILDI_19210: BNILDI_19210 - DUF1425 domain-containing protein, at 3,862,866 to 3,863,243 _19210 BNILDI_19215: hinT - purine nucleoside phosphoramidase, at 3,863,246 to 3,863,605 hinT Position (kb) 3862 3863Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3861.214 kb on - strand, within nagZat 3861.294 kb on - strandat 3861.337 kb on - strandat 3861.415 kb on + strandat 3861.421 kb on + strandat 3861.454 kb on - strandat 3861.548 kb on - strand, within thiKat 3861.581 kb on + strand, within thiKat 3861.582 kb on - strand, within thiKat 3861.582 kb on - strand, within thiKat 3861.706 kb on + strand, within thiKat 3861.729 kb on + strand, within thiKat 3861.730 kb on - strand, within thiKat 3861.743 kb on - strand, within thiKat 3861.784 kb on + strand, within thiKat 3861.892 kb on + strand, within thiKat 3861.892 kb on + strand, within thiKat 3861.896 kb on + strand, within thiKat 3861.896 kb on + strand, within thiKat 3861.897 kb on - strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.987 kb on - strand, within thiKat 3861.988 kb on + strand, within thiKat 3862.143 kb on + strand, within thiKat 3862.147 kb on - strand, within thiKat 3862.213 kb on - strandat 3862.213 kb on - strandat 3862.235 kb on - strandat 3862.359 kb on - strand, within lpoBat 3862.380 kb on - strand, within lpoBat 3862.403 kb on + strand, within lpoBat 3862.403 kb on + strand, within lpoBat 3862.404 kb on - strand, within lpoBat 3862.404 kb on - strand, within lpoBat 3862.404 kb on - strand, within lpoBat 3862.516 kb on + strand, within lpoBat 3862.519 kb on + strand, within lpoBat 3862.585 kb on + strand, within lpoBat 3862.867 kb on + strandat 3862.868 kb on - strandat 3862.875 kb on + strandat 3862.876 kb on - strandat 3863.051 kb on - strand, within BNILDI_19210at 3863.258 kb on - strandat 3863.303 kb on - strand, within hinTat 3863.575 kb on + strandat 3863.590 kb on + strandat 3863.590 kb on + strandat 3863.625 kb on + strandat 3863.669 kb on + strandat 3863.745 kb on + strandat 3863.745 kb on + strandat 3863.746 kb on - strandat 3863.747 kb on + strandat 3863.747 kb on + strandat 3863.748 kb on - strandat 3863.748 kb on - strandat 3863.748 kb on - strandat 3863.748 kb on - strandat 3863.748 kb on - strandat 3863.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,861,214 - nagZ BNILDI_19195 0.82 -0.3
3,861,294 - +0.2
3,861,337 - -1.5
3,861,415 + +0.6
3,861,421 + +0.3
3,861,454 - +0.3
3,861,548 - thiK BNILDI_19200 0.17 -0.2
3,861,581 + thiK BNILDI_19200 0.21 +0.6
3,861,582 - thiK BNILDI_19200 0.21 -0.1
3,861,582 - thiK BNILDI_19200 0.21 +0.3
3,861,706 + thiK BNILDI_19200 0.36 -0.2
3,861,729 + thiK BNILDI_19200 0.39 -1.8
3,861,730 - thiK BNILDI_19200 0.39 +1.6
3,861,743 - thiK BNILDI_19200 0.41 -4.1
3,861,784 + thiK BNILDI_19200 0.46 +1.1
3,861,892 + thiK BNILDI_19200 0.59 -0.3
3,861,892 + thiK BNILDI_19200 0.59 -0.2
3,861,896 + thiK BNILDI_19200 0.59 -0.3
3,861,896 + thiK BNILDI_19200 0.59 -0.5
3,861,897 - thiK BNILDI_19200 0.60 +0.7
3,861,899 + thiK BNILDI_19200 0.60 +0.6
3,861,899 + thiK BNILDI_19200 0.60 +0.5
3,861,899 + thiK BNILDI_19200 0.60 +0.6
3,861,987 - thiK BNILDI_19200 0.70 -0.3
3,861,988 + thiK BNILDI_19200 0.71 -1.0
3,862,143 + thiK BNILDI_19200 0.89 -0.8
3,862,147 - thiK BNILDI_19200 0.90 +0.3
3,862,213 - +0.7
3,862,213 - +0.5
3,862,235 - -1.4
3,862,359 - lpoB BNILDI_19205 0.23 -2.6
3,862,380 - lpoB BNILDI_19205 0.26 +0.4
3,862,403 + lpoB BNILDI_19205 0.30 +0.6
3,862,403 + lpoB BNILDI_19205 0.30 -0.9
3,862,404 - lpoB BNILDI_19205 0.30 -1.9
3,862,404 - lpoB BNILDI_19205 0.30 +0.3
3,862,404 - lpoB BNILDI_19205 0.30 -0.8
3,862,516 + lpoB BNILDI_19205 0.48 -0.3
3,862,519 + lpoB BNILDI_19205 0.48 +0.5
3,862,585 + lpoB BNILDI_19205 0.58 +0.2
3,862,867 + -0.6
3,862,868 - +0.2
3,862,875 + -1.8
3,862,876 - -0.8
3,863,051 - BNILDI_19210 0.49 +0.9
3,863,258 - +1.1
3,863,303 - hinT BNILDI_19215 0.16 +0.8
3,863,575 + -0.5
3,863,590 + -0.3
3,863,590 + +0.1
3,863,625 + +1.7
3,863,669 + +0.1
3,863,745 + -1.8
3,863,745 + +0.9
3,863,746 - -0.2
3,863,747 + +1.0
3,863,747 + +0.3
3,863,748 - +0.1
3,863,748 - -1.1
3,863,748 - +0.1
3,863,748 - +1.4
3,863,748 - +0.7
3,863,749 + -0.9

Or see this region's nucleotide sequence