Strain Fitness in Escherichia coli ECRC62 around BNILDI_19130

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_19120 and ycfZ overlap by 4 nucleotidesycfZ and cobB are separated by 138 nucleotidescobB and nagK are separated by 15 nucleotidesnagK and lolE are separated by 28 nucleotides BNILDI_19120: BNILDI_19120 - DUF3592 domain-containing protein, at 3,843,763 to 3,844,224 _19120 BNILDI_19125: ycfZ - Inner membrane protein YcfZ, at 3,844,221 to 3,845,009 ycfZ BNILDI_19130: cobB - NAD-dependent protein deacylase, at 3,845,148 to 3,845,969 cobB BNILDI_19135: nagK - N-acetylglucosamine kinase, at 3,845,985 to 3,846,896 nagK BNILDI_19140: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 3,846,925 to 3,848,163 lolE Position (kb) 3845 3846Strain fitness (log2 ratio) -3 -2 -1 0 1at 3844.169 kb on + strand, within BNILDI_19120at 3844.252 kb on + strandat 3844.401 kb on - strand, within ycfZat 3844.518 kb on - strand, within ycfZat 3844.625 kb on - strand, within ycfZat 3844.684 kb on + strand, within ycfZat 3844.771 kb on + strand, within ycfZat 3844.839 kb on - strand, within ycfZat 3844.897 kb on + strand, within ycfZat 3845.149 kb on + strandat 3845.150 kb on - strandat 3845.150 kb on - strandat 3845.152 kb on - strandat 3845.229 kb on - strandat 3845.367 kb on - strand, within cobBat 3845.369 kb on - strand, within cobBat 3845.454 kb on - strand, within cobBat 3845.454 kb on - strand, within cobBat 3845.534 kb on + strand, within cobBat 3845.577 kb on + strand, within cobBat 3845.578 kb on - strand, within cobBat 3845.578 kb on - strand, within cobBat 3845.627 kb on + strand, within cobBat 3845.695 kb on + strand, within cobBat 3845.696 kb on - strand, within cobBat 3845.696 kb on - strand, within cobBat 3845.796 kb on - strand, within cobBat 3845.877 kb on - strand, within cobBat 3845.969 kb on + strandat 3845.970 kb on - strandat 3845.970 kb on - strandat 3845.994 kb on + strandat 3846.000 kb on + strandat 3846.001 kb on - strandat 3846.001 kb on - strandat 3846.001 kb on - strandat 3846.075 kb on + strandat 3846.179 kb on + strand, within nagKat 3846.202 kb on - strand, within nagKat 3846.295 kb on + strand, within nagKat 3846.295 kb on + strand, within nagKat 3846.296 kb on - strand, within nagKat 3846.305 kb on - strand, within nagKat 3846.334 kb on + strand, within nagKat 3846.425 kb on + strand, within nagKat 3846.426 kb on - strand, within nagKat 3846.449 kb on - strand, within nagKat 3846.597 kb on + strand, within nagKat 3846.670 kb on + strand, within nagKat 3846.677 kb on + strand, within nagKat 3846.756 kb on + strand, within nagKat 3846.790 kb on + strand, within nagKat 3846.790 kb on + strand, within nagKat 3846.790 kb on + strand, within nagKat 3846.879 kb on - strandat 3846.892 kb on + strandat 3846.893 kb on - strandat 3846.893 kb on - strandat 3846.917 kb on + strandat 3846.918 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,844,169 + BNILDI_19120 0.88 -1.8
3,844,252 + +1.0
3,844,401 - ycfZ BNILDI_19125 0.23 -0.7
3,844,518 - ycfZ BNILDI_19125 0.38 +1.1
3,844,625 - ycfZ BNILDI_19125 0.51 -1.3
3,844,684 + ycfZ BNILDI_19125 0.59 -0.8
3,844,771 + ycfZ BNILDI_19125 0.70 -0.0
3,844,839 - ycfZ BNILDI_19125 0.78 -1.5
3,844,897 + ycfZ BNILDI_19125 0.86 +1.1
3,845,149 + -0.1
3,845,150 - +1.3
3,845,150 - +0.9
3,845,152 - -0.7
3,845,229 - -0.1
3,845,367 - cobB BNILDI_19130 0.27 +0.1
3,845,369 - cobB BNILDI_19130 0.27 -0.1
3,845,454 - cobB BNILDI_19130 0.37 +0.8
3,845,454 - cobB BNILDI_19130 0.37 -1.0
3,845,534 + cobB BNILDI_19130 0.47 -1.7
3,845,577 + cobB BNILDI_19130 0.52 +1.1
3,845,578 - cobB BNILDI_19130 0.52 +0.3
3,845,578 - cobB BNILDI_19130 0.52 +0.2
3,845,627 + cobB BNILDI_19130 0.58 -0.1
3,845,695 + cobB BNILDI_19130 0.67 -2.3
3,845,696 - cobB BNILDI_19130 0.67 +0.0
3,845,696 - cobB BNILDI_19130 0.67 -0.3
3,845,796 - cobB BNILDI_19130 0.79 +0.5
3,845,877 - cobB BNILDI_19130 0.89 +1.2
3,845,969 + -0.5
3,845,970 - -1.0
3,845,970 - +0.2
3,845,994 + +0.5
3,846,000 + -1.7
3,846,001 - +0.1
3,846,001 - +0.2
3,846,001 - -2.4
3,846,075 + -1.2
3,846,179 + nagK BNILDI_19135 0.21 -3.0
3,846,202 - nagK BNILDI_19135 0.24 +0.3
3,846,295 + nagK BNILDI_19135 0.34 -0.7
3,846,295 + nagK BNILDI_19135 0.34 -0.7
3,846,296 - nagK BNILDI_19135 0.34 -0.8
3,846,305 - nagK BNILDI_19135 0.35 +1.0
3,846,334 + nagK BNILDI_19135 0.38 +0.5
3,846,425 + nagK BNILDI_19135 0.48 -0.8
3,846,426 - nagK BNILDI_19135 0.48 +0.2
3,846,449 - nagK BNILDI_19135 0.51 +0.3
3,846,597 + nagK BNILDI_19135 0.67 -1.5
3,846,670 + nagK BNILDI_19135 0.75 -0.1
3,846,677 + nagK BNILDI_19135 0.76 +0.2
3,846,756 + nagK BNILDI_19135 0.85 +0.6
3,846,790 + nagK BNILDI_19135 0.88 +1.0
3,846,790 + nagK BNILDI_19135 0.88 -0.5
3,846,790 + nagK BNILDI_19135 0.88 -0.6
3,846,879 - +0.5
3,846,892 + +0.2
3,846,893 - +0.8
3,846,893 - +1.0
3,846,917 + -1.3
3,846,918 - +0.3

Or see this region's nucleotide sequence