Strain Fitness in Escherichia coli ECRC62 around BNILDI_15850

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyoaE and pdeD are separated by 3 nucleotidespdeD and sdaA are separated by 130 nucleotides BNILDI_15845: yoaE - CNNM family cation transport protein YoaE, at 3,178,455 to 3,180,011 yoaE BNILDI_15850: pdeD - putative cyclic di-GMP phosphodiesterase PdeD, at 3,180,015 to 3,181,613 pdeD BNILDI_15855: sdaA - L-serine ammonia-lyase, at 3,181,744 to 3,183,108 sdaA Position (kb) 3180 3181 3182Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3179.313 kb on + strand, within yoaEat 3179.314 kb on - strand, within yoaEat 3179.383 kb on + strand, within yoaEat 3179.383 kb on + strand, within yoaEat 3179.668 kb on + strand, within yoaEat 3179.669 kb on - strand, within yoaEat 3179.915 kb on - strandat 3180.017 kb on - strandat 3180.042 kb on - strandat 3180.042 kb on - strandat 3180.141 kb on - strandat 3180.163 kb on + strandat 3180.209 kb on + strand, within pdeDat 3180.239 kb on + strand, within pdeDat 3180.274 kb on - strand, within pdeDat 3180.294 kb on - strand, within pdeDat 3180.318 kb on + strand, within pdeDat 3180.394 kb on - strand, within pdeDat 3180.564 kb on - strand, within pdeDat 3180.564 kb on - strand, within pdeDat 3180.631 kb on - strand, within pdeDat 3180.631 kb on - strand, within pdeDat 3180.693 kb on - strand, within pdeDat 3180.722 kb on + strand, within pdeDat 3180.722 kb on + strand, within pdeDat 3180.941 kb on + strand, within pdeDat 3181.022 kb on - strand, within pdeDat 3181.113 kb on - strand, within pdeDat 3181.113 kb on - strand, within pdeDat 3181.291 kb on + strand, within pdeDat 3181.352 kb on + strand, within pdeDat 3181.353 kb on - strand, within pdeDat 3181.434 kb on + strand, within pdeDat 3181.444 kb on - strand, within pdeDat 3181.473 kb on + strandat 3181.582 kb on + strandat 3181.583 kb on - strandat 3181.589 kb on + strandat 3181.681 kb on + strandat 3181.682 kb on - strandat 3181.743 kb on - strandat 3181.743 kb on - strandat 3182.131 kb on - strand, within sdaAat 3182.132 kb on + strand, within sdaAat 3182.174 kb on + strand, within sdaAat 3182.174 kb on + strand, within sdaAat 3182.175 kb on - strand, within sdaAat 3182.175 kb on - strand, within sdaAat 3182.211 kb on - strand, within sdaAat 3182.211 kb on - strand, within sdaAat 3182.213 kb on + strand, within sdaAat 3182.213 kb on + strand, within sdaAat 3182.369 kb on - strand, within sdaAat 3182.422 kb on + strand, within sdaAat 3182.451 kb on + strand, within sdaAat 3182.532 kb on + strand, within sdaA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,179,313 + yoaE BNILDI_15845 0.55 +0.1
3,179,314 - yoaE BNILDI_15845 0.55 +0.1
3,179,383 + yoaE BNILDI_15845 0.60 +0.4
3,179,383 + yoaE BNILDI_15845 0.60 +0.5
3,179,668 + yoaE BNILDI_15845 0.78 +0.7
3,179,669 - yoaE BNILDI_15845 0.78 -1.1
3,179,915 - -0.0
3,180,017 - -2.3
3,180,042 - -0.5
3,180,042 - -0.2
3,180,141 - -0.1
3,180,163 + +1.4
3,180,209 + pdeD BNILDI_15850 0.12 -1.4
3,180,239 + pdeD BNILDI_15850 0.14 -0.1
3,180,274 - pdeD BNILDI_15850 0.16 +0.4
3,180,294 - pdeD BNILDI_15850 0.17 -0.4
3,180,318 + pdeD BNILDI_15850 0.19 +0.2
3,180,394 - pdeD BNILDI_15850 0.24 +1.2
3,180,564 - pdeD BNILDI_15850 0.34 -0.0
3,180,564 - pdeD BNILDI_15850 0.34 -1.2
3,180,631 - pdeD BNILDI_15850 0.39 -0.6
3,180,631 - pdeD BNILDI_15850 0.39 +0.4
3,180,693 - pdeD BNILDI_15850 0.42 -0.0
3,180,722 + pdeD BNILDI_15850 0.44 +0.5
3,180,722 + pdeD BNILDI_15850 0.44 -0.2
3,180,941 + pdeD BNILDI_15850 0.58 -0.3
3,181,022 - pdeD BNILDI_15850 0.63 -0.6
3,181,113 - pdeD BNILDI_15850 0.69 -1.7
3,181,113 - pdeD BNILDI_15850 0.69 -0.4
3,181,291 + pdeD BNILDI_15850 0.80 +0.1
3,181,352 + pdeD BNILDI_15850 0.84 -0.1
3,181,353 - pdeD BNILDI_15850 0.84 +0.2
3,181,434 + pdeD BNILDI_15850 0.89 -1.0
3,181,444 - pdeD BNILDI_15850 0.89 +0.7
3,181,473 + -0.5
3,181,582 + -2.5
3,181,583 - -4.1
3,181,589 + -0.1
3,181,681 + -0.2
3,181,682 - +1.2
3,181,743 - +0.8
3,181,743 - -1.0
3,182,131 - sdaA BNILDI_15855 0.28 -0.2
3,182,132 + sdaA BNILDI_15855 0.28 +0.2
3,182,174 + sdaA BNILDI_15855 0.32 +0.4
3,182,174 + sdaA BNILDI_15855 0.32 +0.4
3,182,175 - sdaA BNILDI_15855 0.32 +0.0
3,182,175 - sdaA BNILDI_15855 0.32 -0.7
3,182,211 - sdaA BNILDI_15855 0.34 +0.6
3,182,211 - sdaA BNILDI_15855 0.34 -1.4
3,182,213 + sdaA BNILDI_15855 0.34 +0.0
3,182,213 + sdaA BNILDI_15855 0.34 -1.6
3,182,369 - sdaA BNILDI_15855 0.46 +0.1
3,182,422 + sdaA BNILDI_15855 0.50 +0.1
3,182,451 + sdaA BNILDI_15855 0.52 -0.0
3,182,532 + sdaA BNILDI_15855 0.58 +0.8

Or see this region's nucleotide sequence