Strain Fitness in Escherichia coli ECRC62 around BNILDI_14570

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaC and wcaD overlap by 26 nucleotideswcaD and wcaE are separated by 10 nucleotideswcaE and wcaF are separated by 15 nucleotides BNILDI_14565: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 2,942,499 to 2,943,716 wcaC BNILDI_14570: wcaD - putative colanic acid polymerase WcaD, at 2,943,691 to 2,944,908 wcaD BNILDI_14575: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 2,944,919 to 2,945,665 wcaE BNILDI_14580: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 2,945,681 to 2,946,229 wcaF Position (kb) 2943 2944 2945Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2942.752 kb on + strand, within wcaCat 2942.829 kb on - strand, within wcaCat 2942.984 kb on - strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.988 kb on - strand, within wcaCat 2943.046 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.323 kb on + strand, within wcaCat 2943.454 kb on - strand, within wcaCat 2943.616 kb on + strandat 2943.690 kb on + strandat 2943.691 kb on - strandat 2943.714 kb on + strandat 2943.718 kb on + strandat 2943.719 kb on - strandat 2943.719 kb on - strandat 2943.719 kb on - strandat 2943.739 kb on + strandat 2943.830 kb on + strand, within wcaDat 2943.830 kb on + strand, within wcaDat 2943.831 kb on - strand, within wcaDat 2943.898 kb on + strand, within wcaDat 2943.931 kb on + strand, within wcaDat 2943.959 kb on - strand, within wcaDat 2943.961 kb on - strand, within wcaDat 2943.973 kb on - strand, within wcaDat 2943.991 kb on - strand, within wcaDat 2944.035 kb on - strand, within wcaDat 2944.965 kb on + strandat 2944.966 kb on - strandat 2945.011 kb on + strand, within wcaEat 2945.012 kb on - strand, within wcaEat 2945.653 kb on + strandat 2945.878 kb on + strand, within wcaF

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,942,752 + wcaC BNILDI_14565 0.21 -0.2
2,942,829 - wcaC BNILDI_14565 0.27 -0.0
2,942,984 - wcaC BNILDI_14565 0.40 +0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.2
2,942,987 + wcaC BNILDI_14565 0.40 -0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.7
2,942,988 - wcaC BNILDI_14565 0.40 -0.6
2,943,046 + wcaC BNILDI_14565 0.45 -2.1
2,943,152 + wcaC BNILDI_14565 0.54 +1.0
2,943,152 + wcaC BNILDI_14565 0.54 +1.0
2,943,323 + wcaC BNILDI_14565 0.68 -1.0
2,943,454 - wcaC BNILDI_14565 0.78 -0.7
2,943,616 + -0.1
2,943,690 + -0.1
2,943,691 - -0.4
2,943,714 + -1.0
2,943,718 + -0.1
2,943,719 - -1.0
2,943,719 - +0.9
2,943,719 - +0.1
2,943,739 + +0.5
2,943,830 + wcaD BNILDI_14570 0.11 -1.6
2,943,830 + wcaD BNILDI_14570 0.11 -0.4
2,943,831 - wcaD BNILDI_14570 0.11 +0.7
2,943,898 + wcaD BNILDI_14570 0.17 +0.0
2,943,931 + wcaD BNILDI_14570 0.20 +0.3
2,943,959 - wcaD BNILDI_14570 0.22 -1.0
2,943,961 - wcaD BNILDI_14570 0.22 +2.5
2,943,973 - wcaD BNILDI_14570 0.23 +0.2
2,943,991 - wcaD BNILDI_14570 0.25 -0.5
2,944,035 - wcaD BNILDI_14570 0.28 -3.6
2,944,965 + -0.2
2,944,966 - -0.7
2,945,011 + wcaE BNILDI_14575 0.12 -3.0
2,945,012 - wcaE BNILDI_14575 0.12 +1.1
2,945,653 + -1.6
2,945,878 + wcaF BNILDI_14580 0.36 -0.3

Or see this region's nucleotide sequence