Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaA and wcaB are separated by 2 nucleotides wcaB and wcaC overlap by 4 nucleotides wcaC and wcaD overlap by 26 nucleotides
BNILDI_14555: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 2,941,172 to 2,942,011
wcaA
BNILDI_14560: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 2,942,014 to 2,942,502
wcaB
BNILDI_14565: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 2,942,499 to 2,943,716
wcaC
BNILDI_14570: wcaD - putative colanic acid polymerase WcaD, at 2,943,691 to 2,944,908
wcaD
Position (kb)
2942
2943
2944 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2941.604 kb on + strand, within wcaA at 2941.605 kb on - strand, within wcaA at 2941.622 kb on + strand, within wcaA at 2941.623 kb on - strand, within wcaA at 2941.623 kb on - strand, within wcaA at 2941.654 kb on + strand, within wcaA at 2941.715 kb on + strand, within wcaA at 2941.745 kb on + strand, within wcaA at 2941.745 kb on + strand, within wcaA at 2941.842 kb on - strand, within wcaA at 2942.223 kb on + strand, within wcaB at 2942.223 kb on + strand, within wcaB at 2942.224 kb on - strand, within wcaB at 2942.256 kb on + strand, within wcaB at 2942.304 kb on + strand, within wcaB at 2942.376 kb on + strand, within wcaB at 2942.673 kb on - strand, within wcaC at 2942.752 kb on + strand, within wcaC at 2942.829 kb on - strand, within wcaC at 2942.984 kb on - strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.988 kb on - strand, within wcaC at 2943.046 kb on + strand, within wcaC at 2943.152 kb on + strand, within wcaC at 2943.152 kb on + strand, within wcaC at 2943.323 kb on + strand, within wcaC at 2943.454 kb on - strand, within wcaC at 2943.616 kb on + strand at 2943.690 kb on + strand at 2943.691 kb on - strand at 2943.714 kb on + strand at 2943.718 kb on + strand at 2943.719 kb on - strand at 2943.719 kb on - strand at 2943.719 kb on - strand at 2943.739 kb on + strand at 2943.830 kb on + strand, within wcaD at 2943.830 kb on + strand, within wcaD at 2943.831 kb on - strand, within wcaD at 2943.898 kb on + strand, within wcaD at 2943.931 kb on + strand, within wcaD at 2943.959 kb on - strand, within wcaD at 2943.961 kb on - strand, within wcaD at 2943.973 kb on - strand, within wcaD at 2943.991 kb on - strand, within wcaD at 2944.035 kb on - strand, within wcaD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,941,604 + wcaA BNILDI_14555 0.51 -0.7 2,941,605 - wcaA BNILDI_14555 0.52 +0.5 2,941,622 + wcaA BNILDI_14555 0.54 -2.9 2,941,623 - wcaA BNILDI_14555 0.54 -1.4 2,941,623 - wcaA BNILDI_14555 0.54 +0.5 2,941,654 + wcaA BNILDI_14555 0.57 -0.0 2,941,715 + wcaA BNILDI_14555 0.65 +0.2 2,941,745 + wcaA BNILDI_14555 0.68 +0.0 2,941,745 + wcaA BNILDI_14555 0.68 -0.2 2,941,842 - wcaA BNILDI_14555 0.80 +0.3 2,942,223 + wcaB BNILDI_14560 0.43 +0.1 2,942,223 + wcaB BNILDI_14560 0.43 -0.2 2,942,224 - wcaB BNILDI_14560 0.43 -0.4 2,942,256 + wcaB BNILDI_14560 0.49 +0.5 2,942,304 + wcaB BNILDI_14560 0.59 +0.1 2,942,376 + wcaB BNILDI_14560 0.74 +0.1 2,942,673 - wcaC BNILDI_14565 0.14 -0.0 2,942,752 + wcaC BNILDI_14565 0.21 -0.2 2,942,829 - wcaC BNILDI_14565 0.27 -0.0 2,942,984 - wcaC BNILDI_14565 0.40 +0.3 2,942,987 + wcaC BNILDI_14565 0.40 +0.2 2,942,987 + wcaC BNILDI_14565 0.40 -0.3 2,942,987 + wcaC BNILDI_14565 0.40 +0.3 2,942,987 + wcaC BNILDI_14565 0.40 +0.7 2,942,988 - wcaC BNILDI_14565 0.40 -0.6 2,943,046 + wcaC BNILDI_14565 0.45 -2.1 2,943,152 + wcaC BNILDI_14565 0.54 +1.0 2,943,152 + wcaC BNILDI_14565 0.54 +1.0 2,943,323 + wcaC BNILDI_14565 0.68 -1.0 2,943,454 - wcaC BNILDI_14565 0.78 -0.7 2,943,616 + -0.1 2,943,690 + -0.1 2,943,691 - -0.4 2,943,714 + -1.0 2,943,718 + -0.1 2,943,719 - -1.0 2,943,719 - +0.9 2,943,719 - +0.1 2,943,739 + +0.5 2,943,830 + wcaD BNILDI_14570 0.11 -1.6 2,943,830 + wcaD BNILDI_14570 0.11 -0.4 2,943,831 - wcaD BNILDI_14570 0.11 +0.7 2,943,898 + wcaD BNILDI_14570 0.17 +0.0 2,943,931 + wcaD BNILDI_14570 0.20 +0.3 2,943,959 - wcaD BNILDI_14570 0.22 -1.0 2,943,961 - wcaD BNILDI_14570 0.22 +2.5 2,943,973 - wcaD BNILDI_14570 0.23 +0.2 2,943,991 - wcaD BNILDI_14570 0.25 -0.5 2,944,035 - wcaD BNILDI_14570 0.28 -3.6
Or see this region's nucleotide sequence