Strain Fitness in Escherichia coli ECRC62 around BNILDI_14565

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaA and wcaB are separated by 2 nucleotideswcaB and wcaC overlap by 4 nucleotideswcaC and wcaD overlap by 26 nucleotides BNILDI_14555: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 2,941,172 to 2,942,011 wcaA BNILDI_14560: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 2,942,014 to 2,942,502 wcaB BNILDI_14565: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 2,942,499 to 2,943,716 wcaC BNILDI_14570: wcaD - putative colanic acid polymerase WcaD, at 2,943,691 to 2,944,908 wcaD Position (kb) 2942 2943 2944Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2941.604 kb on + strand, within wcaAat 2941.605 kb on - strand, within wcaAat 2941.622 kb on + strand, within wcaAat 2941.623 kb on - strand, within wcaAat 2941.623 kb on - strand, within wcaAat 2941.654 kb on + strand, within wcaAat 2941.715 kb on + strand, within wcaAat 2941.745 kb on + strand, within wcaAat 2941.745 kb on + strand, within wcaAat 2941.842 kb on - strand, within wcaAat 2942.223 kb on + strand, within wcaBat 2942.223 kb on + strand, within wcaBat 2942.224 kb on - strand, within wcaBat 2942.256 kb on + strand, within wcaBat 2942.304 kb on + strand, within wcaBat 2942.376 kb on + strand, within wcaBat 2942.673 kb on - strand, within wcaCat 2942.752 kb on + strand, within wcaCat 2942.829 kb on - strand, within wcaCat 2942.984 kb on - strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.987 kb on + strand, within wcaCat 2942.988 kb on - strand, within wcaCat 2943.046 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.152 kb on + strand, within wcaCat 2943.323 kb on + strand, within wcaCat 2943.454 kb on - strand, within wcaCat 2943.616 kb on + strandat 2943.690 kb on + strandat 2943.691 kb on - strandat 2943.714 kb on + strandat 2943.718 kb on + strandat 2943.719 kb on - strandat 2943.719 kb on - strandat 2943.719 kb on - strandat 2943.739 kb on + strandat 2943.830 kb on + strand, within wcaDat 2943.830 kb on + strand, within wcaDat 2943.831 kb on - strand, within wcaDat 2943.898 kb on + strand, within wcaDat 2943.931 kb on + strand, within wcaDat 2943.959 kb on - strand, within wcaDat 2943.961 kb on - strand, within wcaDat 2943.973 kb on - strand, within wcaDat 2943.991 kb on - strand, within wcaDat 2944.035 kb on - strand, within wcaD

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,941,604 + wcaA BNILDI_14555 0.51 -0.7
2,941,605 - wcaA BNILDI_14555 0.52 +0.5
2,941,622 + wcaA BNILDI_14555 0.54 -2.9
2,941,623 - wcaA BNILDI_14555 0.54 -1.4
2,941,623 - wcaA BNILDI_14555 0.54 +0.5
2,941,654 + wcaA BNILDI_14555 0.57 -0.0
2,941,715 + wcaA BNILDI_14555 0.65 +0.2
2,941,745 + wcaA BNILDI_14555 0.68 +0.0
2,941,745 + wcaA BNILDI_14555 0.68 -0.2
2,941,842 - wcaA BNILDI_14555 0.80 +0.3
2,942,223 + wcaB BNILDI_14560 0.43 +0.1
2,942,223 + wcaB BNILDI_14560 0.43 -0.2
2,942,224 - wcaB BNILDI_14560 0.43 -0.4
2,942,256 + wcaB BNILDI_14560 0.49 +0.5
2,942,304 + wcaB BNILDI_14560 0.59 +0.1
2,942,376 + wcaB BNILDI_14560 0.74 +0.1
2,942,673 - wcaC BNILDI_14565 0.14 -0.0
2,942,752 + wcaC BNILDI_14565 0.21 -0.2
2,942,829 - wcaC BNILDI_14565 0.27 -0.0
2,942,984 - wcaC BNILDI_14565 0.40 +0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.2
2,942,987 + wcaC BNILDI_14565 0.40 -0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.3
2,942,987 + wcaC BNILDI_14565 0.40 +0.7
2,942,988 - wcaC BNILDI_14565 0.40 -0.6
2,943,046 + wcaC BNILDI_14565 0.45 -2.1
2,943,152 + wcaC BNILDI_14565 0.54 +1.0
2,943,152 + wcaC BNILDI_14565 0.54 +1.0
2,943,323 + wcaC BNILDI_14565 0.68 -1.0
2,943,454 - wcaC BNILDI_14565 0.78 -0.7
2,943,616 + -0.1
2,943,690 + -0.1
2,943,691 - -0.4
2,943,714 + -1.0
2,943,718 + -0.1
2,943,719 - -1.0
2,943,719 - +0.9
2,943,719 - +0.1
2,943,739 + +0.5
2,943,830 + wcaD BNILDI_14570 0.11 -1.6
2,943,830 + wcaD BNILDI_14570 0.11 -0.4
2,943,831 - wcaD BNILDI_14570 0.11 +0.7
2,943,898 + wcaD BNILDI_14570 0.17 +0.0
2,943,931 + wcaD BNILDI_14570 0.20 +0.3
2,943,959 - wcaD BNILDI_14570 0.22 -1.0
2,943,961 - wcaD BNILDI_14570 0.22 +2.5
2,943,973 - wcaD BNILDI_14570 0.23 +0.2
2,943,991 - wcaD BNILDI_14570 0.25 -0.5
2,944,035 - wcaD BNILDI_14570 0.28 -3.6

Or see this region's nucleotide sequence