Strain Fitness in Escherichia coli ECRC62 around BNILDI_12255

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdeF and ppx are separated by 38 nucleotidesppx and ppk1 are separated by 4 nucleotides BNILDI_12250: pdeF - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,457,408 to 2,459,651 pdeF BNILDI_12255: ppx - exopolyphosphatase, at 2,459,690 to 2,461,231 ppx BNILDI_12260: ppk1 - polyphosphate kinase 1, at 2,461,236 to 2,463,302 ppk1 Position (kb) 2459 2460 2461 2462Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2458.746 kb on + strand, within pdeFat 2458.747 kb on - strand, within pdeFat 2458.747 kb on - strand, within pdeFat 2458.774 kb on - strand, within pdeFat 2458.774 kb on - strand, within pdeFat 2458.797 kb on - strand, within pdeFat 2458.838 kb on + strand, within pdeFat 2458.961 kb on + strand, within pdeFat 2458.977 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.153 kb on + strand, within pdeFat 2459.153 kb on + strand, within pdeFat 2459.157 kb on - strand, within pdeFat 2459.220 kb on - strand, within pdeFat 2459.246 kb on + strand, within pdeFat 2459.433 kb on - strandat 2459.462 kb on - strandat 2459.517 kb on + strandat 2459.518 kb on - strandat 2459.518 kb on - strandat 2459.569 kb on - strandat 2459.570 kb on + strandat 2459.570 kb on + strandat 2459.591 kb on + strandat 2459.758 kb on - strandat 2459.804 kb on - strandat 2459.900 kb on + strand, within ppxat 2459.901 kb on - strand, within ppxat 2459.903 kb on + strand, within ppxat 2459.918 kb on + strand, within ppxat 2459.925 kb on - strand, within ppxat 2459.951 kb on - strand, within ppxat 2459.959 kb on + strand, within ppxat 2459.959 kb on + strand, within ppxat 2460.072 kb on + strand, within ppxat 2460.202 kb on - strand, within ppxat 2460.325 kb on - strand, within ppxat 2460.342 kb on - strand, within ppxat 2460.377 kb on + strand, within ppxat 2460.706 kb on - strand, within ppxat 2460.706 kb on - strand, within ppxat 2460.755 kb on - strand, within ppxat 2460.838 kb on + strand, within ppxat 2460.838 kb on + strand, within ppxat 2460.974 kb on + strand, within ppxat 2460.975 kb on - strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.069 kb on + strand, within ppxat 2461.069 kb on + strand, within ppxat 2461.140 kb on - strandat 2461.152 kb on - strandat 2461.152 kb on - strandat 2461.216 kb on + strandat 2461.265 kb on - strandat 2461.270 kb on + strandat 2461.270 kb on + strandat 2461.271 kb on - strandat 2461.271 kb on - strandat 2461.374 kb on + strandat 2461.375 kb on - strandat 2461.375 kb on - strandat 2461.375 kb on - strandat 2461.538 kb on - strand, within ppk1at 2461.550 kb on + strand, within ppk1at 2461.655 kb on - strand, within ppk1at 2461.688 kb on + strand, within ppk1at 2461.689 kb on - strand, within ppk1at 2461.693 kb on - strand, within ppk1at 2461.693 kb on - strand, within ppk1at 2461.749 kb on + strand, within ppk1at 2461.750 kb on - strand, within ppk1at 2461.760 kb on - strand, within ppk1at 2461.794 kb on + strand, within ppk1at 2461.795 kb on - strand, within ppk1at 2461.838 kb on + strand, within ppk1at 2462.027 kb on + strand, within ppk1at 2462.028 kb on - strand, within ppk1at 2462.073 kb on - strand, within ppk1at 2462.124 kb on - strand, within ppk1at 2462.130 kb on - strand, within ppk1

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,458,746 + pdeF BNILDI_12250 0.60 +0.3
2,458,747 - pdeF BNILDI_12250 0.60 +0.3
2,458,747 - pdeF BNILDI_12250 0.60 -0.5
2,458,774 - pdeF BNILDI_12250 0.61 -0.0
2,458,774 - pdeF BNILDI_12250 0.61 -0.5
2,458,797 - pdeF BNILDI_12250 0.62 +1.1
2,458,838 + pdeF BNILDI_12250 0.64 -0.1
2,458,961 + pdeF BNILDI_12250 0.69 -1.0
2,458,977 + pdeF BNILDI_12250 0.70 -0.1
2,459,099 + pdeF BNILDI_12250 0.75 +0.6
2,459,099 + pdeF BNILDI_12250 0.75 +0.4
2,459,099 + pdeF BNILDI_12250 0.75 +0.2
2,459,100 - pdeF BNILDI_12250 0.75 +0.2
2,459,100 - pdeF BNILDI_12250 0.75 -0.8
2,459,100 - pdeF BNILDI_12250 0.75 +0.1
2,459,100 - pdeF BNILDI_12250 0.75 +2.0
2,459,100 - pdeF BNILDI_12250 0.75 -0.4
2,459,153 + pdeF BNILDI_12250 0.78 -0.1
2,459,153 + pdeF BNILDI_12250 0.78 -2.3
2,459,157 - pdeF BNILDI_12250 0.78 +0.4
2,459,220 - pdeF BNILDI_12250 0.81 +0.7
2,459,246 + pdeF BNILDI_12250 0.82 +0.6
2,459,433 - +0.0
2,459,462 - -0.3
2,459,517 + -0.2
2,459,518 - +1.0
2,459,518 - +0.7
2,459,569 - +1.2
2,459,570 + +0.2
2,459,570 + -0.0
2,459,591 + -0.2
2,459,758 - +2.7
2,459,804 - -0.2
2,459,900 + ppx BNILDI_12255 0.14 -0.1
2,459,901 - ppx BNILDI_12255 0.14 +1.4
2,459,903 + ppx BNILDI_12255 0.14 -0.3
2,459,918 + ppx BNILDI_12255 0.15 -0.4
2,459,925 - ppx BNILDI_12255 0.15 -0.6
2,459,951 - ppx BNILDI_12255 0.17 -3.8
2,459,959 + ppx BNILDI_12255 0.17 +0.4
2,459,959 + ppx BNILDI_12255 0.17 -0.9
2,460,072 + ppx BNILDI_12255 0.25 -0.3
2,460,202 - ppx BNILDI_12255 0.33 -0.0
2,460,325 - ppx BNILDI_12255 0.41 +1.0
2,460,342 - ppx BNILDI_12255 0.42 -0.2
2,460,377 + ppx BNILDI_12255 0.45 -3.5
2,460,706 - ppx BNILDI_12255 0.66 -1.1
2,460,706 - ppx BNILDI_12255 0.66 +0.7
2,460,755 - ppx BNILDI_12255 0.69 +0.3
2,460,838 + ppx BNILDI_12255 0.74 -1.6
2,460,838 + ppx BNILDI_12255 0.74 -0.6
2,460,974 + ppx BNILDI_12255 0.83 +0.3
2,460,975 - ppx BNILDI_12255 0.83 +0.9
2,461,066 + ppx BNILDI_12255 0.89 +0.1
2,461,066 + ppx BNILDI_12255 0.89 +1.6
2,461,066 + ppx BNILDI_12255 0.89 -0.0
2,461,069 + ppx BNILDI_12255 0.89 +1.7
2,461,069 + ppx BNILDI_12255 0.89 -0.6
2,461,140 - +0.1
2,461,152 - +0.7
2,461,152 - +0.1
2,461,216 + -0.0
2,461,265 - -1.0
2,461,270 + -0.2
2,461,270 + -0.5
2,461,271 - -1.4
2,461,271 - +0.7
2,461,374 + -0.6
2,461,375 - -0.1
2,461,375 - -1.9
2,461,375 - -0.1
2,461,538 - ppk1 BNILDI_12260 0.15 -0.9
2,461,550 + ppk1 BNILDI_12260 0.15 -0.5
2,461,655 - ppk1 BNILDI_12260 0.20 -1.0
2,461,688 + ppk1 BNILDI_12260 0.22 -0.6
2,461,689 - ppk1 BNILDI_12260 0.22 -0.6
2,461,693 - ppk1 BNILDI_12260 0.22 -0.7
2,461,693 - ppk1 BNILDI_12260 0.22 -0.8
2,461,749 + ppk1 BNILDI_12260 0.25 -0.9
2,461,750 - ppk1 BNILDI_12260 0.25 -1.0
2,461,760 - ppk1 BNILDI_12260 0.25 -0.5
2,461,794 + ppk1 BNILDI_12260 0.27 +0.8
2,461,795 - ppk1 BNILDI_12260 0.27 -0.4
2,461,838 + ppk1 BNILDI_12260 0.29 +0.4
2,462,027 + ppk1 BNILDI_12260 0.38 -1.0
2,462,028 - ppk1 BNILDI_12260 0.38 -2.1
2,462,073 - ppk1 BNILDI_12260 0.40 -1.9
2,462,124 - ppk1 BNILDI_12260 0.43 -0.5
2,462,130 - ppk1 BNILDI_12260 0.43 -0.5

Or see this region's nucleotide sequence