Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemW and rdgB overlap by 8 nucleotides rdgB and yggU are separated by 7 nucleotides yggU and yggT overlap by 4 nucleotides yggT and yggS are separated by 17 nucleotides
BNILDI_09685: hemW - radical SAM family heme chaperone HemW, at 1,949,206 to 1,950,342
hemW
BNILDI_09690: rdgB - XTP/dITP diphosphatase, at 1,950,335 to 1,950,928
rdgB
BNILDI_09695: yggU - DUF167 domain-containing protein, at 1,950,936 to 1,951,226
yggU
BNILDI_09700: yggT - osmotic shock tolerance protein YggT, at 1,951,223 to 1,951,789
yggT
BNILDI_09705: yggS - pyridoxal phosphate homeostasis protein, at 1,951,807 to 1,952,511
yggS
Position (kb) 1950
1951
1952 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1949.976 kb on + strand, within hemW at 1950.054 kb on + strand, within hemW at 1950.166 kb on + strand, within hemW at 1950.167 kb on - strand, within hemW at 1950.186 kb on - strand, within hemW at 1950.314 kb on + strand at 1950.337 kb on - strand at 1950.515 kb on - strand, within rdgB at 1950.558 kb on + strand, within rdgB at 1950.559 kb on - strand, within rdgB at 1950.559 kb on - strand, within rdgB at 1950.560 kb on + strand, within rdgB at 1950.560 kb on + strand, within rdgB at 1950.561 kb on - strand, within rdgB at 1950.678 kb on + strand, within rdgB at 1950.678 kb on + strand, within rdgB at 1950.679 kb on - strand, within rdgB at 1950.707 kb on + strand, within rdgB at 1950.933 kb on + strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.942 kb on - strand at 1951.124 kb on + strand, within yggU at 1951.125 kb on - strand, within yggU at 1951.140 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.208 kb on + strand at 1951.208 kb on + strand at 1951.208 kb on + strand at 1951.226 kb on + strand at 1951.271 kb on + strand at 1951.272 kb on - strand at 1951.275 kb on - strand at 1951.303 kb on - strand, within yggT at 1951.303 kb on - strand, within yggT at 1951.408 kb on - strand, within yggT at 1951.568 kb on - strand, within yggT at 1951.581 kb on + strand, within yggT at 1951.746 kb on + strand at 1951.746 kb on + strand at 1951.747 kb on - strand at 1951.783 kb on + strand at 1951.890 kb on + strand, within yggS at 1951.990 kb on + strand, within yggS at 1951.994 kb on + strand, within yggS at 1952.038 kb on + strand, within yggS at 1952.119 kb on - strand, within yggS
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,949,976 + hemW BNILDI_09685 0.68 +0.3 1,950,054 + hemW BNILDI_09685 0.75 +1.2 1,950,166 + hemW BNILDI_09685 0.84 -2.3 1,950,167 - hemW BNILDI_09685 0.85 -0.5 1,950,186 - hemW BNILDI_09685 0.86 -0.0 1,950,314 + +0.1 1,950,337 - -0.1 1,950,515 - rdgB BNILDI_09690 0.30 -1.1 1,950,558 + rdgB BNILDI_09690 0.38 +0.2 1,950,559 - rdgB BNILDI_09690 0.38 +0.9 1,950,559 - rdgB BNILDI_09690 0.38 -1.8 1,950,560 + rdgB BNILDI_09690 0.38 -0.2 1,950,560 + rdgB BNILDI_09690 0.38 -1.2 1,950,561 - rdgB BNILDI_09690 0.38 -0.2 1,950,678 + rdgB BNILDI_09690 0.58 -0.2 1,950,678 + rdgB BNILDI_09690 0.58 -1.1 1,950,679 - rdgB BNILDI_09690 0.58 -0.7 1,950,707 + rdgB BNILDI_09690 0.63 +3.8 1,950,933 + -0.8 1,950,938 - +0.1 1,950,938 - -0.8 1,950,938 - +0.8 1,950,938 - +1.5 1,950,942 - +1.9 1,951,124 + yggU BNILDI_09695 0.65 -0.7 1,951,125 - yggU BNILDI_09695 0.65 +1.7 1,951,140 + yggU BNILDI_09695 0.70 -0.4 1,951,180 + yggU BNILDI_09695 0.84 -0.3 1,951,180 + yggU BNILDI_09695 0.84 -0.0 1,951,180 + yggU BNILDI_09695 0.84 +1.6 1,951,208 + +1.1 1,951,208 + +0.1 1,951,208 + -1.2 1,951,226 + +0.2 1,951,271 + +0.2 1,951,272 - +0.4 1,951,275 - +2.0 1,951,303 - yggT BNILDI_09700 0.14 -0.9 1,951,303 - yggT BNILDI_09700 0.14 -0.1 1,951,408 - yggT BNILDI_09700 0.33 +1.3 1,951,568 - yggT BNILDI_09700 0.61 -0.2 1,951,581 + yggT BNILDI_09700 0.63 -1.3 1,951,746 + +1.7 1,951,746 + +0.4 1,951,747 - +0.1 1,951,783 + -0.2 1,951,890 + yggS BNILDI_09705 0.12 -0.7 1,951,990 + yggS BNILDI_09705 0.26 -0.2 1,951,994 + yggS BNILDI_09705 0.27 +1.0 1,952,038 + yggS BNILDI_09705 0.33 -0.0 1,952,119 - yggS BNILDI_09705 0.44 -0.5
Or see this region's nucleotide sequence