Strain Fitness in Escherichia coli ECRC62 around BNILDI_08420

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_08410 and apaH are separated by 77 nucleotidesapaH and apaG are separated by 6 nucleotidesapaG and rsmA are separated by 2 nucleotidesrsmA and pdxA overlap by 4 nucleotides BNILDI_08410: BNILDI_08410 - Dihydrofolate reductase, at 1,680,396 to 1,680,986 _08410 BNILDI_08415: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 1,681,064 to 1,681,906 apaH BNILDI_08420: apaG - Co2+/Mg2+ efflux protein ApaG, at 1,681,913 to 1,682,290 apaG BNILDI_08425: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 1,682,293 to 1,683,114 rsmA BNILDI_08430: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 1,683,111 to 1,684,100 pdxA Position (kb) 1681 1682 1683Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1681.049 kb on + strandat 1681.050 kb on - strandat 1681.050 kb on - strandat 1681.050 kb on - strandat 1681.050 kb on - strandat 1681.050 kb on - strandat 1681.065 kb on + strandat 1681.185 kb on + strand, within apaHat 1681.186 kb on - strand, within apaHat 1681.187 kb on + strand, within apaHat 1681.188 kb on - strand, within apaHat 1681.190 kb on - strand, within apaHat 1681.246 kb on - strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.323 kb on + strand, within apaHat 1681.433 kb on - strand, within apaHat 1681.445 kb on - strand, within apaHat 1681.609 kb on + strand, within apaHat 1681.667 kb on - strand, within apaHat 1681.730 kb on + strand, within apaHat 1681.731 kb on - strand, within apaHat 1681.831 kb on + strandat 1681.844 kb on - strandat 1681.870 kb on - strandat 1681.896 kb on + strandat 1681.896 kb on + strandat 1681.897 kb on - strandat 1681.897 kb on - strandat 1681.907 kb on - strandat 1681.914 kb on + strandat 1682.047 kb on - strand, within apaGat 1682.197 kb on + strand, within apaGat 1682.266 kb on + strandat 1682.295 kb on - strandat 1682.416 kb on - strand, within rsmAat 1682.636 kb on - strand, within rsmAat 1682.636 kb on - strand, within rsmAat 1682.768 kb on + strand, within rsmAat 1683.249 kb on - strand, within pdxAat 1683.283 kb on - strand, within pdxA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,681,049 + -0.0
1,681,050 - -0.0
1,681,050 - +2.1
1,681,050 - -0.4
1,681,050 - +0.2
1,681,050 - -1.9
1,681,065 + -0.3
1,681,185 + apaH BNILDI_08415 0.14 +1.7
1,681,186 - apaH BNILDI_08415 0.14 -2.1
1,681,187 + apaH BNILDI_08415 0.15 -1.7
1,681,188 - apaH BNILDI_08415 0.15 -2.4
1,681,190 - apaH BNILDI_08415 0.15 -1.8
1,681,246 - apaH BNILDI_08415 0.22 -4.1
1,681,323 + apaH BNILDI_08415 0.31 -2.3
1,681,323 + apaH BNILDI_08415 0.31 -0.9
1,681,323 + apaH BNILDI_08415 0.31 -2.0
1,681,323 + apaH BNILDI_08415 0.31 -0.7
1,681,433 - apaH BNILDI_08415 0.44 -1.5
1,681,445 - apaH BNILDI_08415 0.45 -1.1
1,681,609 + apaH BNILDI_08415 0.65 -3.4
1,681,667 - apaH BNILDI_08415 0.72 +0.4
1,681,730 + apaH BNILDI_08415 0.79 -0.7
1,681,731 - apaH BNILDI_08415 0.79 -3.5
1,681,831 + -0.9
1,681,844 - -0.9
1,681,870 - -0.6
1,681,896 + -1.3
1,681,896 + -2.6
1,681,897 - -1.8
1,681,897 - -1.7
1,681,907 - -1.3
1,681,914 + -1.4
1,682,047 - apaG BNILDI_08420 0.35 -0.1
1,682,197 + apaG BNILDI_08420 0.75 -0.9
1,682,266 + -0.8
1,682,295 - -0.3
1,682,416 - rsmA BNILDI_08425 0.15 -1.1
1,682,636 - rsmA BNILDI_08425 0.42 +0.3
1,682,636 - rsmA BNILDI_08425 0.42 -0.3
1,682,768 + rsmA BNILDI_08425 0.58 -0.9
1,683,249 - pdxA BNILDI_08430 0.14 -1.1
1,683,283 - pdxA BNILDI_08430 0.17 -2.6

Or see this region's nucleotide sequence