Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaU and kefF are separated by 107 nucleotides kefF and kefC overlap by 8 nucleotides kefC and BNILDI_08410 are separated by 80 nucleotides BNILDI_08410 and apaH are separated by 77 nucleotides
BNILDI_08395: yaaU - Putative metabolite transport protein YaaU, at 1,676,491 to 1,677,822
yaaU
BNILDI_08400: kefF - glutathione-regulated potassium-efflux system oxidoreductase KefF, at 1,677,930 to 1,678,460
kefF
BNILDI_08405: kefC - glutathione-regulated potassium-efflux system protein KefC, at 1,678,453 to 1,680,315
kefC
BNILDI_08410: BNILDI_08410 - Dihydrofolate reductase, at 1,680,396 to 1,680,986
_08410
BNILDI_08415: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 1,681,064 to 1,681,906
apaH
Position (kb)
1678
1679
1680
1681 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1677.500 kb on + strand, within yaaU at 1677.520 kb on + strand, within yaaU at 1677.521 kb on - strand, within yaaU at 1677.616 kb on - strand, within yaaU at 1677.616 kb on - strand, within yaaU at 1677.616 kb on - strand, within yaaU at 1677.623 kb on + strand, within yaaU at 1677.631 kb on - strand, within yaaU at 1677.670 kb on - strand, within yaaU at 1677.676 kb on - strand, within yaaU at 1677.701 kb on + strand at 1677.792 kb on + strand at 1677.793 kb on - strand at 1677.857 kb on + strand at 1677.857 kb on + strand at 1677.858 kb on - strand at 1677.864 kb on + strand at 1677.865 kb on - strand at 1677.866 kb on + strand at 1677.881 kb on + strand at 1677.881 kb on + strand at 1677.888 kb on - strand at 1677.929 kb on + strand at 1677.929 kb on + strand at 1677.946 kb on - strand at 1678.044 kb on + strand, within kefF at 1678.417 kb on - strand at 1678.418 kb on + strand at 1678.418 kb on + strand at 1678.419 kb on - strand at 1678.464 kb on + strand at 1678.465 kb on - strand at 1678.465 kb on - strand at 1678.486 kb on + strand at 1678.512 kb on - strand at 1678.614 kb on + strand at 1678.656 kb on + strand, within kefC at 1678.772 kb on + strand, within kefC at 1678.772 kb on + strand, within kefC at 1678.773 kb on - strand, within kefC at 1678.773 kb on - strand, within kefC at 1679.040 kb on - strand, within kefC at 1679.054 kb on - strand, within kefC at 1679.419 kb on - strand, within kefC at 1679.703 kb on - strand, within kefC at 1679.770 kb on - strand, within kefC at 1679.791 kb on + strand, within kefC at 1679.959 kb on + strand, within kefC at 1680.064 kb on + strand, within kefC at 1680.316 kb on + strand at 1680.414 kb on + strand at 1680.414 kb on + strand at 1680.467 kb on + strand, within BNILDI_08410 at 1680.467 kb on + strand, within BNILDI_08410 at 1680.472 kb on - strand, within BNILDI_08410 at 1680.473 kb on + strand, within BNILDI_08410 at 1681.049 kb on + strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.065 kb on + strand at 1681.185 kb on + strand, within apaH at 1681.186 kb on - strand, within apaH at 1681.187 kb on + strand, within apaH at 1681.188 kb on - strand, within apaH at 1681.190 kb on - strand, within apaH at 1681.246 kb on - strand, within apaH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,677,500 + yaaU BNILDI_08395 0.76 +0.9 1,677,520 + yaaU BNILDI_08395 0.77 -0.5 1,677,521 - yaaU BNILDI_08395 0.77 -0.3 1,677,616 - yaaU BNILDI_08395 0.84 +0.6 1,677,616 - yaaU BNILDI_08395 0.84 +0.2 1,677,616 - yaaU BNILDI_08395 0.84 -0.2 1,677,623 + yaaU BNILDI_08395 0.85 +0.4 1,677,631 - yaaU BNILDI_08395 0.86 -0.6 1,677,670 - yaaU BNILDI_08395 0.89 +0.7 1,677,676 - yaaU BNILDI_08395 0.89 +0.2 1,677,701 + -0.9 1,677,792 + +0.9 1,677,793 - -0.4 1,677,857 + +0.2 1,677,857 + +0.5 1,677,858 - +0.8 1,677,864 + -0.5 1,677,865 - +1.6 1,677,866 + -0.3 1,677,881 + -2.2 1,677,881 + +0.1 1,677,888 - -0.4 1,677,929 + +0.4 1,677,929 + -0.1 1,677,946 - +0.3 1,678,044 + kefF BNILDI_08400 0.21 +0.6 1,678,417 - -0.2 1,678,418 + +0.4 1,678,418 + -0.3 1,678,419 - +0.0 1,678,464 + -1.2 1,678,465 - -0.1 1,678,465 - +0.9 1,678,486 + +0.2 1,678,512 - +0.0 1,678,614 + +0.2 1,678,656 + kefC BNILDI_08405 0.11 +0.1 1,678,772 + kefC BNILDI_08405 0.17 -0.8 1,678,772 + kefC BNILDI_08405 0.17 +0.0 1,678,773 - kefC BNILDI_08405 0.17 +0.1 1,678,773 - kefC BNILDI_08405 0.17 -0.4 1,679,040 - kefC BNILDI_08405 0.32 +0.1 1,679,054 - kefC BNILDI_08405 0.32 -3.4 1,679,419 - kefC BNILDI_08405 0.52 +0.2 1,679,703 - kefC BNILDI_08405 0.67 -0.9 1,679,770 - kefC BNILDI_08405 0.71 +1.3 1,679,791 + kefC BNILDI_08405 0.72 +1.0 1,679,959 + kefC BNILDI_08405 0.81 -0.4 1,680,064 + kefC BNILDI_08405 0.86 -0.8 1,680,316 + -0.5 1,680,414 + +1.8 1,680,414 + -0.1 1,680,467 + BNILDI_08410 0.12 -0.7 1,680,467 + BNILDI_08410 0.12 -0.2 1,680,472 - BNILDI_08410 0.13 -0.1 1,680,473 + BNILDI_08410 0.13 +1.0 1,681,049 + -0.0 1,681,050 - -0.0 1,681,050 - +2.1 1,681,050 - -0.4 1,681,050 - +0.2 1,681,050 - -1.9 1,681,065 + -0.3 1,681,185 + apaH BNILDI_08415 0.14 +1.7 1,681,186 - apaH BNILDI_08415 0.14 -2.1 1,681,187 + apaH BNILDI_08415 0.15 -1.7 1,681,188 - apaH BNILDI_08415 0.15 -2.4 1,681,190 - apaH BNILDI_08415 0.15 -1.8 1,681,246 - apaH BNILDI_08415 0.22 -4.1
Or see this region's nucleotide sequence